2001 Tercera División play-offs
The 2001 Tercera División play-offs to Segunda División B from Tercera División were the final playoffs for the promotion from 2000–01 Tercera División to 2001–02 Segunda División B. The first four teams in each group took part in the play-off.
Format
The 68 participating teams were divided into 5 series each made up of 4 groups in the category, with the exception of Series E , which was only formed by Group XII . Each series was divided into 4 groups formed by a 1st, a 2nd, a 3rd and a 4th classified from each group, which played a double-round playoff. Each victory was equivalent to 3 points, the tie to 1 point and the defeat to 0 points. The champion of each group obtained the promotion to Second Division B.The distribution of each series was as follows:
Teams for 2000–01 Play-Offs
Group I – Galicia  | Group II – Asturias  | Group III – Cantabria  | Group IV – Basque Country  | Group V – Catalonia  |
| 1st Celta de Vigo B | 1st Marino de Luanco | 1st SD Noja | 1st SD Lemona | 1st CF Gavà |
| 2nd CD Endesa As Pontes | 2nd Real Oviedo B | 2nd SD Laredo | 2nd Real Sociedad B | 2nd CF Balaguer |
| 3rd Ponte Ourense CF | 3rd UP Langreo | 3rd SD Textil Escudo | 3rd Sestao River Club | 3rd CE Europa |
| 4th UD Xove Lago | 4th CD Lealtad | 4th CD Bezana | 4th CD Aurrerá Ondarroa | 4th Palamós CF |
Group XI – Balearic Islands  | Group XII – Canary Islands  | Group XIII – Region of Murcia  | Group XIV – Extremadura  | Group XV – Navarre and La Rioja   |
| 1st CD Atlético Baleares | 1st UD Lanzarote | 1st CF Ciudad de Murcia | 1st CD Díter Zafra | 1st CD Logroñés |
| 3rd CF Villafranca | 2nd SD Tenisca | 2nd Orihuela CF | 2nd UD Mérida | 2nd CD Mirandés |
| 4th CD Manacor | 3rd UD Las Palmas B | 3rd Yeclano CF | 3rd UP Plasencia | 3rd CD Alfaro |
| 5th CD Constancia | 4th CD San Isidro | 4th Lorca CF | 4th CP Cacereño | 4th CD Izarra |