The first round matches of the 2007–08 UEFA Cup were played on 20 September and 4 October 2007, which narrowed clubs down to 40 teams in preparation for the group stage. !colspan="5"|Group 1 !colspan="5"|Group 2 !colspan="5"|Group 3 !colspan="5"|Group 4 !colspan="5"|Group 5 !colspan="5"|Group 6 !colspan="5"|Group 7 !colspan="5"|Group 8
Panathinaikos won 5–1 on aggregate. ---- Nürnberg 2–2 Rapid București on aggregate. Nürnberg won on away goals. ---- Tottenham Hotspur won 7–2 on aggregate. ---- Lokomotiv Moscow won 5–1 on aggregate. ---- Helsingborg won 8–6 on aggregate. ---- Atlético Madrid won 9–0 on aggregate. ---- Hamburg won 4–1 on aggregate. ---- Zürich won 4–2 on aggregate. ---- Austria Wien won 4–2 on aggregate. ---- Dinamo București 2–2 Elfsborg on aggregate. Elfsborg won on away goals. ---- Aberdeen 1–1 Dnipro Dnipropetrovsk on aggregate. Aberdeen won on away goals. ---- Toulouse 1–1 CSKA Sofia on aggregate. Toulouse won on away goals. ---- Aalborg BK 2–2 Sampdoria on aggregate. Aalborg BK won on away goals. ---- Spartak Moscow won 8–1 on aggregate. ---- Sparta Prague 0–0 Odense on aggregate. Sparta Prague won 4–3 on penalties. ---- Basel won 8–1 on aggregate. ---- Dinamo Zagreb 3–3 Ajax on aggregate. Dinamo Zagreb won on away goals. ---- Villarreal won 6–1 on aggregate. ---- Panionios won 2–1 on aggregate. ---- Zenit Saint Petersburg won 4–1 on aggregate. ---- Copenhagen won 3–2 on aggregate. ---- AEK Athens won 3–1 on aggregate. ---- Hapoel Tel Aviv won 1–0 on aggregate. ---- Brann 2–2 Club Brugge on aggregate. Brann won on away goals. ---- Bayern Munich won 3–0 on aggregate. ---- Galatasaray won 7–4 on aggregate. ---- Everton won 4–3 on aggregate. ---- Getafe 3–3 Twente on aggregate. Getafe won on away goals. ---- Red Star Belgrade won 2–0 on aggregate. ---- Rennes won 4–3 on aggregate. ---- Palermo 1–1 Mladá Boleslav on aggregate. Mladá Boleslav won 4–2 on penalties. ---- Anderlecht won 2–1 on aggregate. ---- Fiorentina 2–2 Groningen on aggregate. Fiorentina won 4–3 on penalties. ---- Bolton Wanderers won 2–1 on aggregate. ---- Aris 2–2 Real Zaragoza on aggregate. Aris won on away goals. ---- Larissa won 3–2 on aggregate. ---- Bordeaux won 4–3 on aggregate. ---- AZ won 1–0 on aggregate. ---- Braga won 5–2 on aggregate. ---- Bayer Leverkusen won 5–4 on aggregate.
