World record | Jon Drummond, Andre Cason, Dennis Mitchell, Leroy Burrell | 37.40 | Stuttgart, Germany | 21 August 1993 |
Championship record | Jon Drummond, Andre Cason, Dennis Mitchell, Leroy Burrell | 37.40 | Stuttgart, Germany | 21 August 1993 |
World Leading | Racers Track Club Daniel Bailey, Yohan Blake, Mario Forsythe, Usain Bolt | 37.46 | London, Great Britain | 25 July 2009 |
African Record | Osmond Ezinwa, Olapade Adeniken, Francis Obikwelu, Davidson Ezinwa | 37.94 | Athens, Greece | 9 August 1997 |
Asian Record | Naoki Tsukahara, Shingo Suetsugu, Shinji Takahira, Nobuharu Asahara | 38.03 | Osaka, Japan | 1 September 2007 |
North American Record | Jon Drummond, Andre Cason, Dennis Mitchell, Leroy Burrell | 37.40 | Stuttgart, Germany | 21 August 1993 |
South American record | André da Silva, Claudinei da Silva, Vicente de Lima, Édson Ribeiro | 37.90 | Sydney, Australia | 30 September 2000 |
European Record | Dwain Chambers, Jason Gardener, Darren Campbell, Marlon Devonish | 37.73 | Seville, Spain | 29 August 1999 |
Oceanian Record | Paul Henderson, Tim Jackson, Steve Brimacombe, Damien Marsh | 38.17 | Gothenburg, Sweden | 12 August 1995 |
Rank | Heat | Nation | Athletes | Time | Notes |
| 2 | | Simeon Williamson, Tyrone Edgar, Marlon Devonish, Harry Aikines-Aryeetey | 38.11 | Q, SB |
| 1 | | Darrel Brown, Marc Burns, Keston Bledman, Richard Thompson | 38.47 | Q |
| 3 | | Roberto Donati, Simone Collio, Emanuele Di Gregorio, Fabio Cerutti | 38.52 | Q, SB |
| 1 | | Masashi Eriguchi, Naoki Tsukahara, Shinji Takahira, Kenji Fujimitsu | 38.53 | Q |
| 1 | | Ronald Pognon, Martial Mbandjock, Pierre-Alexis Pessonneaux, Christophe Lemaitre | 38.59 | q, SB |
| 2 | | Hank Palmer, Oluseyi Smith, Jared Connaughton, Bryan Barnett | 38.60 | Q, SB |
| 3 | | Lerone Clarke, Michael Frater, Steve Mullings, Dwight Thomas | 38.60 | Q |
| 1 | | Vicente de Lima, Sandro Viana, Basílio de Moraes, José Carlos Moreira | 38.72 | q, SB |
| 3 | | Anthony Alozie, Josh Ross, Aaron Rouge-Serret, Matt Davies | 38.93 | SB |
10 | 2 | | Gregory Sedoc, Caimin Douglas, Guus Hoogmoed, Patrick van Luijk | 38.95 | SB |
11 | 2 | | Dany Gonçalves, Arnaldo Abrantes, Ricardo Monteiro, Francis Obikwelu | 39.25 | |
12 | 1 | | Pascal Mancini, Marc Schneeberger, Reto Schenkel, Marco Cribari | 39.47 | |
13 | 2 | | Nana Kofi Samm, Tanko Braimah, Seth Amoo, Aziz Zakari | 39.61 | SB |
14 | 1 | | Hannes Dreyer, Leigh Julius, Thuso Mpuang, L. J. van Zyl | 39.71 | |
15 | 3 | | Apinan Sukaphai, Wachara Sondee, Suppachai Chimdee, Sittichai Suwonprateep | 39.73 | |
| 2 | | Terrence Trammell, Michael Rodgers, Shawn Crawford, Darvis Patton | | Illegal handover |
| 3 | | Tobias Unger, Marius Broening, Alexander Kosenkow, Martin Keller | | |
| 3 | | Michał Bielczyk, Dariusz Kuć, Mikołaj Lewański, Robert Kubaczyk | | |