This is a knockout stage of the 52 teams that did not receive byes to the [|first round]. First legs: 12–14 February 2010; Second legs: 26–28 February 2010. ---- APR won 3 - 2 on aggregate and advanced to the first round. ---- Djoliba won 1 - 0 on aggregate and advanced to the first round. ---- Aggregate 2 - 2. ASC Linguère won the penalty shootout and advanced to the first round. ---- Ismaily won 2 - 0 on aggregate and advanced to the first round. ---- US Stade Tamponnaise won 5 - 2 on aggregate and advanced to the first round. ---- Africa Sports advanced to the first round after the Benin representative was withdrawn. ---- Raja Casablanca won 4 -2 on aggregate and advanced to the first round. ---- Atlético Petróleos Luanda won 9 - 3 on aggregate and advanced to the first round. ---- Aggregate 2 - 2. Club Africain advanced on the away goals rule to the first round. ---- JS Kabylie won 5 – 1 on aggregate and advanced to the first round. ---- Gazelle FC won 3 - 2 on aggregate and advanced to the first round. ---- Al-Merreikh won 4 - 2 on aggregate and advanced to the first round. ---- Espérance ST won 5 - 4 on aggregate and advanced to the first round. ---- ASFA Yennega won 6 - 1 on aggregate and advanced to the first round. ---- Curepipe Starlight won 3 - 2 on aggregate and advanced to the first round. ---- Aggregate 2 - 2. Gaborone United advanced on the away goals rule to the first round. ---- Saint Eloi Lupopo won 4 - 2 on aggregate and advanced to the first round. ---- Gunners won 6 - 1 on aggregate and advanced to the first round. ---- Ittihad won 8 - 1 on aggregate and advanced to the first round. ---- Difaa El Jadida won 3 - 0 on aggregate and advanced to the first round. ---- Zanaco won 4 - 1 on aggregate and advanced to the first round. ---- Union Douala advanced to the first round after the São Tomé and Príncipe representative was withdrawn. ---- ES Sétif won 4 - 3 on aggregate and advanced to the first round. ---- Aggregate 4 - 4. Tiko United won the penalty shootout and advanced to the first round. ---- Supersport United won 5 - 3 and advanced to the first round. ---- Ferroviário Maputo won 9 - 4 on aggregate and advanced to the first round. ----
First round
This is a knock-out stage of 32 teams; the 26 teams advancing from the [|preliminary round], and 6 teams that received byes to this round. First legs: 19–21 March 2010; Second legs: 2–5 April 2010. ---- TP Mazembe won 2 - 1 on aggregate and advanced to the [|second round]. ---- Aggregate 1 - 1. Djoliba won the penalty shootout and advanced to the second round. ---- Ismaily won 3 - 2 on aggregate and advanced to the second round. ---- Al-Hilal Omdurman won 4 - 1 on aggregate and advanced to the second round. ---- Atlético Petróleos Luanda won 2 - 1 on aggregate and advanced to the second round. ---- JS Kabylie won 2 - 1 on aggregate and advanced to the second round. ---- Al-Merreikh won 3 - 1 on aggregate and advanced to the second round. ---- Espérance ST won 7 -2 on aggregate and advanced to the second round. ---- Gaborone United won 6 - 0 on aggregate and advanced to the second round. ---- Dynamos won 2 - 0 on aggregate and advanced to the second round. ---- Al-Ahly won 2 - 1 on aggregate and advanced to the second round. ---- Aggregate 2 - 2. Ittihad won the penalty shootout and advanced to the second round. ---- Zanaco won 2 - 1 on aggregate and advanced to the second round. ---- ES Sétif won 7 - 0 on aggregate and advanced to the second round. ---- Aggregate 3 - 3. Heartland advanced on the away goals rule to the second round. ---- Supersport United won 3 - 2 on aggregate and advanced to the second round. ----
Second round
This is a knock-out stage of the 16 teams that advanced from the first round; winners will advance to the group stage, with the losers advancing to the CAF Confederation Cup. First legs: 23–25 April 2010; Second legs 7–9 May 2010. ---- TP Mazembe won 4 – 0 on aggregate and advanced to the group stage. ---- Ismaily won 4 – 1 on aggregate and advanced to the group stage. ---- JS Kabylie won 3 – 2 on aggregate and advanced to the group stage. ---- Espérance ST won 4 - 1 on aggregate and advanced to the group stage. ---- Dynamos won 4 - 2 on aggregate and advanced to the group stage. ---- Al-Ahly won 3 – 2 on aggregate and advanced to the group stage. ---- ES Sétif won 3 - 2 on aggregate and advanced to the group stage. ---- Heartland won 4 – 2 on aggregate and advanced to the group stage.
