| Rank | Heat | Lane | Nation | Athletes | Time | Notes |
| 1 | 2 | 3 | | Phara Anacharsis, Elea Mariama Diarra, Lenora Guion Firmin, Floria Guei | 3:29.03 | Q |
| 2 | 1 | 4 | | Yuilya Olishevska, Darya Prystupa, Nataliya Pyhyda, Alina Lohvynenko | 3:29.09 | Q |
| 3 | 2 | 6 | | Eilidh Child, Kelly Massey, Nicola Sanders, Shana Cox | 3:29.96 | Q |
| 4 | 2 | 7 | | Jitka Bartoničková, Denisa Rosolová, Zuzana Bergrová, Zuzana Hejnová | 3:30.86 | Q |
| 5 | 2 | 5 | | Olga Tovarnova, Tatyana Veshkurova, Liliya Molgacheva, Yuliya Terekhova | 3:31.35 | q |
| 6 | 1 | 3 | | Esther Cremer, Janin Lindenberg, Christiane Klopsch, Fabienne Kohlmann | 3:31.38 | Q |
| 7 | 1 | 1 | | Elena Mirela Lavric, Alina Panainte, Bianca Razor, Angela Moroșanu | 3:31.48 | Q |
| 8 | 2 | 1 | | Agata Bednarek, Justyna Święty, Iga Baumgart, Anna Jesień | 3:31.50 | q |
| 8 | 1 | 2 | | Maryna Boika, Iryna Khliustava, Yulia Yurenya, Ilona Usovich | 3:31.50 | |
| 10 | 2 | 2 | | Chiara Bazzoni, Maria Enrica Spacca, Libania Grenot, Marta Milani | 3:31.64 | |
| 1 | 8 | | Sema Apak, Merve Aydın, Meliz Redif, Pınar Saka | 3:34.70 | Doping |
| 11 | 1 | 6 | | Irene Høvik Helgesen, Nina Katrine Brandt, Line Kloster, Martine Eikemo Borge | 3:37.74 | |
| 12 | 1 | 7 | | Aauri Bokesa, Natalia Romero, Begoña Garrido, Andrea Díez | 3:38.00 | |
| 13 | 2 | 8 | | Carla Tavares, Carolina Duarte, Vera Barbosa, Joceline Monteiro | 3:40.79 | |
| 14 | 2 | 4 | | Jenni Kivioja, Ella Räsänen, Ilona Punkkinen, Emma Millard | 3:40.97 | |
| 1 | 5 | | Claire Bergin, Joanne Cuddihy, Marian Heffernan, Michelle Carey | | |
