Rank | Heat | Lane | Nation | Athletes | Time | Notes |
1 | 1 | 8 | | Kazuma Oseto, Akiyuki Hashimoto, Aska Cambridge, Kazuki Kanamori | 39.01 | Q, WJL |
2 | 3 | 4 | | Emmanuel Stephens, Chijindu Ujah, Thomas Holligan, Adam Gemili | 39.09 | Q, SB |
3 | 3 | 5 | | Jevaughn Minzie, Odean Skeen, Senoj-Jay Givans, Jazeel Murphy | 39.19 | Q, SB |
4 | 2 | 6 | | Cameron Burrell, Aldrich Bailey, Arman Hall, Arthur Delaney | 39.25 | Q, SB |
5 | 1 | 4 | | Leandro de Araújo, Yuri Monteiro, Renato dos Santos Junior, Rodrigo Rocha | 39.29 | Q, AJ |
6 | 1 | 7 | | Kamil Bijowski, Przemysław Słowikowski, Karol Zalewski, Adam Jabłoński | 39.31 | q, NJ |
7 | 1 | 9 | | Ben Jaworski, Hugh Donovan, Nicholas Hough, Matthew Bertacco | 39.34 | q, AJ |
8 | 2 | 7 | | Anthony Farrington, Blake Bartlett, Shane Jones, Stephen Newbold | 39.48 | Q, NJ |
9 | 1 | 6 | | Ruttanapon Sowan, Kritsada Namsuwan, Jaran Sathoengram, Jirapong Meenapra | 39.68 | SB |
10 | 1 | 5 | | Chukwudike Harry, Okeudo Jonathan Nmaju, Mamus Emuobonuvie, Martins Ogierakhia | 39.86 | SB |
11 | 1 | 3 | | Angus Julies, Siphelo Ngquboza, Pieter Conradie, Fana Mofokeng | 39.87 | SB |
12 | 2 | 9 | | Jesse Berkley, Jonathan Holder, Ashron Sobers, Jonathan Farinha | 40.01 | |
13 | 2 | 3 | | Robert Polkowski, Patrick Domogala, Maximilian Ruth, Jonas Lohmann | 40.08 | SB |
14 | 2 | 8 | | Alessandro Pino, Eseosa Desalu, Giacomo Tortu, Giovanni Galbieri | 40.41 | SB |
15 | 3 | 4 | | Devon Rittinger, Drelan Bramwell, Mobolade Ajomale, Calvin Arsenault | 40.58 | SB |
16 | 2 | 5 | | Morten Dalgaard Madsen, Andreas Trajkovski, Emil Strom, Frederik Thomsen | 40.60 | NJ |
17 | 3 | 6 | | Adrián Pérez, Daniel Mazón, Óscar Husillos, Rodrigo De la Oliva | 41.08 | SB |
18 | 3 | 7 | | Husain Mohamed Hasan, Ahmed Mohamed Alshawoosh, Husain Sameer Mubarak, Ali Omar Abdulla Al Doseri | 41.09 | |
| 3 | 8 | | Guy-Elphège Anouman, Pierre Chalus, Mickael-Meba Zeze, Ken Romain | | |
| 3 | 3 | | Brahian Peña, Yanier Bello, Silvan Wicki, Bastien Mouthon | | |
| 2 | 4 | | Miika Wynne-Ellis, Markus Koivula, Samuli Lehtinen, Eetu Rantala | | |
| 3 | 2 | | Marek Bakalár, Michal Desensky, Jan Jirka, Zdenek Stromšík | | |