Rank | Heat | Lane | Nation | Athletes | Time | Notes |
1 | 3 | 5 | | Lalonde Gordon, Jarrin Solomon, Renny Quow, Machel Cedenio | 3:02.09 | Q, SB |
2 | 1 | 4 | | Michael Mathieu, Demetrius Pinder, Alonzo Russell, Ramon Miller | 3:02.18 | Q |
3 | 1 | 8 | | Pedro Luiz de Oliveira, Wagner Cardoso, Hederson Estefani, Hugo de Sousa | 3:02.23 | Q, SB |
4 | 3 | 1 | | Dylan Borlée, Jonathan Borlée, Julien Watrin, Kevin Borlée | 3:02.41 | Q, SB |
5 | 2 | 8 | | David Verburg, Kyle Clemons, Jeremy Wariner, Brycen Spratling | 3:02.81 | Q |
6 | 2 | 2 | | Javere Bell, Rusheen McDonald, Nathon Allen, Javon Francis | 3:02.98 | Q, SB |
7 | 2 | 3 | | Leaname Maotoanong, Zacharia Kamberuka, Onkabetse Nkobolo, Isaac Makwala | 3:03.08 | q, SB |
8 | 3 | 2 | | Conrad Williams, Delano Williams, Jack Green, George Caddick | 3:03.28 | q, SB |
9 | 1 | 2 | | William Collazo, Raidel Acea, Adrian Chacón, Yoandys Lescay | 3:03.50 | SB |
10 | 2 | 5 | | Mame-Ibra Anne, Teddy Atine-Venel, Thomas Jordier, Mamoudou Hanne | 3:03.88 | SB |
11 | 1 | 1 | | Karol Zalewski, Michał Pietrzak, Łukasz Krawczuk, Rafał Omelko | 3:05.13 | |
12 | 2 | 4 | | Alexander Lerionka Sampao, Boniface Ontuga Mweresa, Michael Cheren Makan, Alphas Leken Kishoyian | 3:05.92 | |
13 | 3 | 3 | | Omar Longart, Alberth Bravo, José Meléndez, Freddy Mezones | 3:06.15 | SB |
13 | 1 | 7 | | Mazen Al Yasen, Ali Al-Deraan, Mohammed Obaid Al-Salhi, Yousef Ahmed Masrahi | 3:06.15 | SB |
15 | 1 | 3 | | Kamghe Gaba, Eric Krüger, Johannes Trefz, Thomas Schneider | 3:06.20 | |
16 | 3 | 7 | | Alexander Beck, Craig Burns, Luke Stevens, Iain Douglas | 3:06.37 | |
17 | 2 | 1 | | Naoki Kobayashi, Kentaro Sato, Julian Jrummi Walsh, Takamasa Kitagawa | 3:06.38 | SB |
18 | 1 | 5 | | Jhon Perlaza, Diego Palomeque, Carlos Lemos, Bernardo Baloyes | 3:06.48 | SB |
19 | 2 | 6 | | Orukpe Eraiyokan, Robert Simmons, Cristian Morton, Miles Ukaoma | 3:06.92 | |
20 | 3 | 6 | | Craig Lynch, Thomas Barr, Brian Murphy, Richard Morrissey | 3:07.11 | SB |
21 | 3 | 4 | | Philip Osei, Mike Robertson, Daniel Harper, Tremaine Harris | 3:07.80 | SB |
22 | 1 | 6 | | Pau Fradera, Samuel García, Mark Ujakpor, Lucas Bua | 3:08.49 | SB |
23 | 3 | 8 | | Luguelín Santos, Juander Santos, Yon Soriano, Winder Cuevas | 3:12.55 | |
| 2 | 7 | | Liemarvin Bonevacia, Bjorn Blauwhof, Obed Martis, Terrence Agard | | |
Rank | Lane | Nation | Athletes | Time | Notes |
1 | 4 | | Kacper Kozłowski, Michał Pietrzak, Mateusz Fórmański, Rafał Omelko | 3:03.23 | |
2 | 6 | | William Collazo, Raidel Acea, Adrian Chacón, Yoandys Lescay | 3:03.73 | SB |
3 | 1 | | Kamghe Gaba, Eric Krüger, Johannes Trefz, Thomas Schneider | 3:04.90 | SB |
4 | 2 | | Craig Burns, Iain Douglas, Luke Stevens, Samuel Baird | 3:05.13 | SB |
| 5 | | Mame-Ibra Anne, Teddy Atine-Venel, Thomas Jordier, Mamoudou Hanne | | |
| 3 | | Alexander Lerionka Sampao, Boniface Ontuga Mweresa, Joseph Poghishio Loshangar, Raymond Kibet | | |
| 7 | | | | |
| 8 | | | | |
Rank | Lane | Nation | Athletes | Time | Notes |
| 6 | | David Verburg, Tony McQuay, Jeremy Wariner, LaShawn Merritt | 2:58.43 | WL |
| 3 | | Ramon Miller, Michael Mathieu, Steven Gardiner, Chris Brown | 2:58.91 | SB |
| 8 | | Dylan Borlée, Julien Watrin, Jonathan Borlée, Kevin Borlée | 2:59.33 | NR |
4 | 7 | | Rusheen McDonald, Edino Steele, Ricardo Chambers, Javon Francis | 3:00.23 | SB |
5 | 5 | | Pedro Luiz de Oliveira, Wagner Cardoso, Hederson Estefani, Hugo de Sousa | 3:00.96 | SB |
6 | 2 | | Conrad Williams, Rabah Yousif, Jack Green, George Caddick | 3:01.51 | SB |
7 | 4 | | Lalonde Gordon, Jarrin Solomon, Renny Quow, Machel Cedenio | 3:03.10 | |
8 | 1 | | Onkabetse Nkobolo, Zacharia Kamberuka, Leaname Maotoanong, Isaac Makwala | 3:03.73 | |