Rank | Heat | Nation | Athletes | Time | Notes |
1 | 2 | | Akeem Haynes, Aaron Brown, Brendon Rodney, Andre De Grasse | 38.21 | Q |
2 | 2 | | Marvin Bracy, Mike Rodgers, Ronnie Baker, Justin Gatlin | 38.22 | Q |
3 | 3 | | Chijindu Ujah, Zharnel Hughes, Adam Gemili, Daniel Talbot | 38.32 | Q, SB |
4 | 1 | | Giovanni Codrington, Churandy Martina, Solomon Bockarie, Hensley Paulina | 38.71 | Q, SB |
5 | 1 | | Mo Youxue, Xie Zhenye, Su Bingtian, Zhang Peimeng | 38.97 | Q, SB |
6 | 1 | | Trae Williams, Tom Gamble, Alexander Hartmann, Nicholas Andrews | 39.09 | q, SB |
7 | 2 | | Marvin René, Stuart Dutamby, Mickael-Meba Zeze, Jimmy Vicaut | 39.10 | q, SB |
8 | 2 | | Julian Reus, Robin Erewa, Alexander Kosenkow, Sven Knipphals | 39.11 | SB |
9 | 3 | | Mario Burke, Ramon Gittens, Nicholas Deshong, Burkheart Ellis Jr | 39.26 | Q, SB |
10 | 1 | | Warren Fraser, Shavez Hart, Cliff Resias, Adrian Griffith | 39.36 | SB |
11 | 1 | | Keston Bledman, Kyle Greaux, Emmanuel Callender, Dan-Neil Telesford | 39.44 | SB |
12 | 3 | | Takumi Masuda, Yuma Saito, Jun Yamashita, Kenta Oshima | 39.52 | SB |
13 | 3 | | Christopher Valdez, Yohandris Andújar, Mayobanex de Óleo, Juan Aroldi Santos | 39.56 | SB |
14 | 3 | | Harlyn Pérez, Yordan O'Farrill, Yoan Medina, Reynier Mena | 39.67 | SB |
15 | 2 | | Héctor Ruiz, Iván Moreno, Juan Carlos Alanis, César Ramírez | 39.71 | SB |
16 | 3 | | Hakeem Huggins, Lestrod Roland, Allistar Clarke, Brijesh Lawrence | 39.81 | SB |
17 | 2 | | Dominik Kopeć, Przemysław Słowikowski, Artur Zaczek, Grzegorz Zimniewicz | 39.84 | SB |
| 2 | | Fabio Cerutti, Eseosa Desalu, Federico Cattaneo, Filippo Tortu | | R170.7 |
| 3 | | António Cesar Rodrigues, Bruno de Barros, Derick Silva, Vítor Hugo dos Santos | | R163.3 |
| 1 | | Chavaughn Walsh, Daniel Bailey, Jared Jarvis, Tahir Walsh | | |
| 1 | | Everton Clarke, Kemar Bailey-Cole, Jevaughn Minzie, Yohan Blake | | |
Rank | Lane | Nation | Athletes | Time | Notes |
1 | 5 | | Moriba Morain, Emmanuel Callender, Kyle Greaux, Dan-Neil Telesford | 39.04 | SB, *WC |
2 | 3 | | Roy Schmidt, Robin Erewa, Julian Reus, Aleixo-Platini Menga | 39.15 | *WC |
3 | 4 | | Warren Fraser, Shavez Hart, Cliff Resias, Adrian Griffith | 39.18 | SB, *WC |
4 | 7 | | Christopher Valdez, Yohandris Andújar, Carlos Jorge, Juan Aroldi Santos | 39.57 | |
5 | 8 | | Harlyn Pérez, Yordan O'Farrill, Yoan Medina, Reynier Mena | 39.90 | |
6 | 2 | | Héctor Ruiz, Iván Moreno, Juan Carlos Alanis, César Ramírez | 39.98 | |
7 | 6 | | Takumi Masuda, Yuma Saito, Jun Yamashita, Takeshi Fujiwara | 40.31 | |
8 | 1 | | Hakeem Huggins, Lestrod Roland, Allistar Clarke, Brijesh Lawrence | 41.07 | |
Rank | Lane | Nation | Athletes | Time | Notes |
| 5 | | Leshon Collins, Mike Rodgers, Ronnie Baker, Justin Gatlin | 38.43 | *WC |
| 8 | | Mario Burke, Ramon Gittens, Nicholas Deshong, Burkheart Ellis Jr | 39.18 | , *WC |
| 7 | | Tang Xingqiang, Xie Zhenye, Su Bingtian, Liang Jinsheng | 39.22 | *WC |
4 | 2 | | Trae Williams, Tom Gamble, Alexander Hartmann, Nicholas Andrews | 39.73 | *WC |
5 | 1 | | Marvin René, Jimmy Vicaut, Mickael-Meba Zeze, Stuart Dutamby | 39.83 | *WC |
| 4 | | Akeem Haynes, Aaron Brown, Brendon Rodney, Andre De Grasse | | |
| 3 | | Giovanni Codrington, Churandy Martina, Solomon Bockarie, Hensley Paulina | | |
| 6 | | Chijindu Ujah, Zharnel Hughes, Daniel Talbot, Ojie Edoburun | | |