Rank | Heat | Nation | Athletes | Time | Notes |
1 | 1 | | Mikiah Brisco, Ashley Henderson, Dezerea Bryant, Aleia Hobbs | 42.51 | Q, WL, *WC |
2 | 3 | | Lisa Marie Kwayie, Lisa Mayer, Gina Lückenkemper, Rebekka Haase | 43.03 | Q, SB, *WC |
3 | 1 | | Andressa Fidelis, Lorraine Martins, Franciela Krasucki, Vitoria Cristina Rosa | 43.07 | Q, SB, *WC |
4 | 3 | | Sherone Simpson, Natasha Morrison, Shashalee Forbes, Jonielle Smith | 43.08 | Q, SB, *WC |
5 | 3 | | Sally Pearson, Maddie Coates, Riley Day, Naa Anang | 43.19 | q, SB, *WC |
6 | 3 | | Johanelis Herrera Abreu, Gloria Hooper, Anna Bongiorni, Irene Siragusa | 43.40 | q, SB, *WC |
7 | 1 | | Kamaria Durant, Michelle-Lee Ahye, Reyare Thomas, Semoy Hackett | 43.67 | SB, *WC |
8 | 3 | | Rima Kashafutdinova, Elina Mikhina, Svetlana Golendova, Olga Safronova | 43.71 | *WC |
9 | 2 | | Astrid Glenner-Frandsen, Ida Karstoft, Mette Graversgaard, Mathilde Kramer | 43.90 | Q, NR, *WC |
10 | 3 | | Ciara Neville, Gina Akpe-Moses, Rhasidat Adeleke, Patience Jumbo Gula | 44.02 | SB |
11 | 2 | | Flings Owusu-Agyapong, Gemma Acheampong, Persis William-Mensah, Halutie Hor | 44.12 | Q, SB, *WC |
12 | 2 | | On-Uma Chattha, Khanrutai Pakdee, Tassaporn Wannakit, Supawan Thipat | 44.24 | |
13 | 1 | | Anna Doi, Miku Yamada, Ichiko Iki, Naoka Miyake | 44.24 | SB |
14 | 1 | | Hanna Plotitsyna, Hrystyna Stuy, Yana Kachur, Mariya Mokrova | 44.55 | SB |
15 | 3 | | Klára Seidlová, Marcela Pírková, Jana Slaninová, Lucie Domská | 44.67 | SB |
16 | 3 | | Marina Poroso, Gabriela Suárez, Narcisa Landazuri, Ángela Tenorio | 44.74 | SB |
17 | 2 | | Joy Udo-Gabriel, Patience Okon George, Favour Ofili, Rosemary Chukwuma | 45.07 | SB |
| 1 | | Kamila Ciba, Katarzyna Sokólska, Marika Popowicz-Drapała, Ewa Swoboda | | |
| 1 | | Rose Xeyi, Tamzin Thomas, Justine Palframan, Tebogo Mamathu | | |
| 2 | | Maroussia Paré, Sarah Richard Mingas, Cynthia Leduc, Carolle Zahi | | |
| 2 | | Kristal Awuah, Desiree Henry, Ashleigh Nelson, Daryll Neita | | |
| 1 | | Liang Xiaojing, Wei Yongli, Kong Lingwei, Ge Manqi | | R170.6 |
| 2 | | Farah Jacques, Crystal Emmanuel, Shaina Harrison, Khamica Bingham | | R170.7 |
Rank | Lane | Nation | Athletes | Time | Notes | Points |
| 5 | | Mikiah Brisco, Ashley Henderson, Dezerea Bryant, Aleia Hobbs | 43.27 | | 8 |
| 8 | | Gayon Evans, Natasha Morrison, Sashalee Forbes, Jonielle Smith | 43.29 | | 7 |
| 7 | | Lisa Marie Kwayie, Alexandra Burghardt, Gina Lückenkemper, Rebekka Haase | 43.68 | | 6 |
4 | 6 | | Ana Carolina Azevedo, Lorraine Martins, Franciela Krasucki, Vitoria Cristina Rosa | 43.75 | | 5 |
5 | 2 | | Johanelis Herrera Abreu, Gloria Hooper, Anna Bongiorni, Irene Siragusa | 44.29 | | 4 |
6 | 3 | | Sally Pearson, Nana Adoma Owusu-Afriyie, Riley Day, Naa Anang | 44.62 | | 3 |
7 | 9 | | Flings Owusu-Agyapong, Gemma Acheampong, Persis William-Mensah, Halutie Hor | 44.77 | | 2 |
8 | 4 | | Louise Østergaard, Astrid Glenner-Frandsen, Mette Graversgaard, Mathilde Kramer | 45.32 | | 1 |