Dutta was born in Kolkata, India. He attended St. Patrick's Higher Secondary School in Asansol and obtained his MBBS degree from Christian Medical College & Hospital, Vellore, graduating as the Best Outgoing Student in 1982. After a year as a research assistant at the Indian Institute of Chemical Biology, Kolkata, he enrolled at Rockefeller University in New York City for doctoral studies with Hidesaburo Hanafusa to work on viral oncology. He obtained his Ph.D. in 1989. He joined the laboratory of Bruce Stillman at Cold Spring Harbor Laboratory to do postdoctoral research on the cell-cycle regulation of DNA replication. In 1992 he began a residency in Anatomic Pathology at Brigham and Women's Hospital, Harvard Medical School, where he went on to become an assistant and then associate professor of pathology. In 2003, he was appointed the Harry F. Byrd Professor of Biochemistry and Molecular Genetics at the University of Virginia's School of Medicine. Dutta discovered how the cell cycle factor p21 interacts with and inhibits cyclin-dependent kinases and PCNA, identifying the cyclin-binding Cy or RXL motif that are also used by cdk to identify substrates for phosphorylation. He discovered how the interaction of p21, Cdt1, Set8 and other important cell-cycle regulators with PCNA triggers their ubiquitylation by CRL4-Cdt2 and subsequent proteasomal degradation. His laboratory cloned the cDNAs of many human DNA replication initiation factors and their regulators and identified how the geminin-Cdt1 balance is important for the prevention of over-replication in human cells. These discoveries explained how a new experimental anti-cancer drug MLN4924 caused over-replication of the DNA and DNA damage leading to cancer cell death. The lab discovered the E2 in the Fanconi Anemia pathway, UBE2T, that is now known to cause Fanconi Anemia. In an early adoption of genomic technologies as part of the ENCODE pilot project, Dutta's lab molecularly identified domains of human chromosomes that replicated early or late in S phase and showed that they corresponded to chromosomal domains with active or repressive epigenetic marks respectively. He confirmed that most origins of replication in human cells appear to be zones with multiple initiation sites that are each used inefficiently in a given cell in a population of cells. The laboratory discovered tens of thousands of extrachromosomal circles of DNA in normal and cancerous cells and in tissues in humans, mice and chickens and somatically mosaic chromosomal microdeletions in some of the hotspots of microDNA production. The microDNA are released into the circulation and will add to the repertoire of cell-free-circulating DNA that is being used for liquid biopsy in cancers and in prenatal noninvasive genetic diagnoses. Finally, the lab has discovered how deletion of MCM9 and ASF1a genes in certain human cancers make the cancer cells susceptible to DNA damage-inducing therapy and that the USP46 deubiquitinase should be targeted for therapy of cancers caused by human papillomavirus. In the area of noncoding RNAs, Dutta discovered the role of microRNAs like miR-206 and of long noncoding RNAs like H19 and MUNC in promoting skeletal muscle differentiation and regeneration after injury. The group has identified the roles of several microRNAs in tumorigenesis and scores of long noncoding RNAs whose expression levels predict the outcome of gliomas and has suggested that lncRNA expression patterns could be used for prognostic purposes. Dutta has identified a novel family of short RNAs derived from the processing of tRNAs, called tRFs. tRFs are emerging to be versatile regulators of cell function, with some of them regulating cellular gene expression by microRNA-like pathways even though they are not generated by enzymes that normally generate microRNAs
Ranbaxy Research Award in Basic Biomedical Science
Harry F. Byrd Professor, Harrison Distinguished Professor, Millipub Award for papers with >1000 citations, Team Science Award, Distinguished Scientist Award, University of Virginia
Outstanding Investigator Award, American Society for Investigative Pathology
Mark Brothers Award, Indiana University School of Medicine