The bottle is normally designed to have a special shoulder radius to push out all air from the inside of the bottle when a sample solution is being filled into the bottle. According to the EPAStandardMethods for the Examination of Water and Wastewater or the Standard Methods 5210, the BOD bottle should include a ground-glassstopper and a flared mouth which form a water seal preventing the air from the outside of the bottle coming in. The Standard Method 5210 also recommends to use a paper, a foil or a plastic cup to cap over the mouth of the bottle reducing the evaporation during the incubation. Generally, the side of the BOD bottle is permanently screened with white writing area, and is printed with a specific number; both for the aid of the sample identification. ;Stopper There are two kinds of stopper: the Glass Pennyhead and the Glass Robotic stopper. ;Sizes There are many sizes of the BOD bottle. The dose of the mixture of the solution is related to the size of the bottle. For the Standard Methods 5210, the BOD bottle “having 60 mL or greater capacity ” is mentioned as one of the apparatus for the BOD test. However, 60 mL BOD bottle is recommended by EPA Method 405.1 stating that it is more convenient using this size of the bottle.
Materials
is a material being specified in the Standard Methods 5210 of the BOD5 test. The glass bottles are manufactured from Type 1borosilicate glass. ;A black BOD bottle A black BOD bottle is coated with PVC plastic that block visible light. The black bottle is used in marinephotosynthesis projects which needs the comparison of oxygen in the light and dark condition. ;Disposable BOD Bottle or Carbonaceous Biochemical Oxygen Demand Bottle It is a carbon-coated polyethylene terephthalate bottle. The bottle is solely manufactured by Environmental Express in Charleston, SC. The bottle is lightweight, unbreakable, and recyclable. Since the bottle is designed for single-use, it eliminates any potential for cross-contamination between samples. The bottle does not require any resources nor energy for cleaning and rinsing as it is disposable. CBOD bottle is also claimed to be cheaper, and cause less contamination in the sample solution than the conventional- BOD bottle.
