Bayview was determined by geography, with railway lines running along the level ground parallel to the northern shore of Lake Ontario increasingly constrained by the Niagara Escarpment as they approach the western end of the lake. There are three major railway lines operated by the Canadian National Railway which intersect at Bayview: ;CN/Metrolinx Oakville Subdivision ;CN Dundas Subdivision ;CN Grimsby Subdivision In addition to the CN lines intersecting at Bayview, the Canadian Pacific Railway has a line which crosses over the CN lines on a grade-separated overpass. The CP Hamilton Subdivision, running from Hamilton to Guelph Junction, joins the CN Grimsby Subdivision immediately east of Bayview at Hamilton Junction.
Train operations
The CN Dundas Subdivision and CN Oakville Subdivision constitute CN's main line running from Chicago, Illinois, to Toronto. The CN Grimsby Subdivision provides CN with access to United States railways in Buffalo, New York, thus making it a busy junction for freight traffic. The population growth in the Greater Toronto Area since 1967 has resulted in the establishment of GO TransitLakeshore West line commuter rail service on the Grimsby and Oakville Subdivisions. Additionally, Via Rail Canada provides intercity passenger rail services on all three CN lines. The CP Hamilton Subdivision links the industrial city of Hamilton and U.S. railway interchange traffic further south in Buffalo with the railway's mainline from Detroit, Michigan to Toronto at Guelph Junction. This line carries exclusively freight traffic and is not as heavily used as the CN lines. Daily traffic density can vary. Upwards of 45 freight trains and 30-40 passenger trains per day can pass through Bayview Junction on both the CN and CP tracks.
Railfanning
Several locations in and around the Royal Botanical Gardens provide viewing of the railway lines.
A pedestrian bridge crosses the CN Oakville Subdivision immediately east of the junction. It is located in the Laking Gardens section of the Royal Botanical Gardens.
A location near a parking lot adjacent to the gardens allows one to view CP Hamilton West, providing a view of the CP Hamilton Subdivision.
A location partway down the pedestrian staircase on the Hamilton side of the York St. high level bridge permits viewing of GO Transit commuter trains and CP yard trains crossing the Desjardins Canal, as well as a daily Amtrak train to New York City, as well as Norfolk Southern, Southern Ontario Railway, and CN trains.
A walkway in the Royal Botanical Gardens Rock Garden provides a view of the double-track Dundas Subdivision.
