As at 10 November 2005, the museum has a collection of 25 trams, 24 of which formerly operated on the Brisbane tram network. The 25th tram in the museum's collection ran in Sydney. The museum also has two single-deck Brisbane trolley-buses built on MF2B chassis by Sunbeam of Wolverhampton, England; fleet numbers 1 and 34. These are on static display. A number of trams in the museum's collection are operated on a short length of demonstration track 250 metres in length. The oldest operational tram in the museum's collection is No. 47, a "California Combination" or "Matchbox" tram, built in 1901. The newest tram in the collection is No. 554 a "Four Motor" tram built by the Brisbane City Council after the Paddington tram depot fire, and which entered service in 1964. Other operational trams include a 10 bench "Toastrack" tram No. 65, a small centre-aisle or "Baby Dreadnought" tram No. 99, No. 341, a "Dropcentre" tram and another "Four Motor" tram No. 429. A selection of these trams operate each Sunday afternoon, although operations are curtailed in the event of wet weather. The museum's collection of support vehicles are all former Brisbane City Council Tramways Department vehicles and include a tower wagon used for repairing overhead wires, and a Scammellbreakdown recovery truck which was formerly used as an artillery towing truck in the Second World War. The museum houses an extensive photographic collection of Brisbane's tramway and street transport heritage, together with tickets and uniforms worn by staff of the tramway operators, a feature of which were the unusual "Foreign legion" caps worn by drivers and conductors until 1961 and inspectors until more recently. The museum is also notable for other elements of the former Brisbane system in its collection. The museum's depots are composed of bays from the former Ipswich Road tram depot. The museum's direct current substation has been built from equipment retrieved from former Brisbane tramway substations. An elevated signal cabin which stood at the corner of Brunswick and Wickham Streets, Fortitude Valley has also been preserved. The museum's very short section of track has been recovered from Ipswich Road depot, Gregory Terrace and Ipswich Road between Moorooka and Salisbury. In keeping with the practice of the Brisbane City Council Tramways Department, the museum has constructed its track in mass concrete, that is, the track is laid in concrete, rather than on sleepers and ballast. The cost of such mass concrete trackwork being one the reason why the museum has been unable to extend their length of track after numerous efforts. The museum also houses a unique three-way set of points that was originally located at Light Street depot. These points can be found outside the museum's main depot shed.
