CCDC113


Coiled-coil domain-containing protein 113 also known as HSPC065, GC16Pof6842 and GC16P044152, is a protein that in humans is encoded by the CCDC113 gene. The human CCDC113 gene is located on chromosome 16q21 and encodes 5,304 base pairs of mRNA and 377 amino acids.

Gene

CCDC113 is located on chromosome 16q21 and encodes two distinct isoforms with isoform 2 containing one less alternate in-frame exon compared to the full length protein, isoform 1. Isoform 1 is composed of 5304 base pairs of mRNA which form the 9 exons that make up the coding sequence.
CCDC113, located between nucleotides 58283840 and 58317740 on chromosome 16, is surrounded between antisense genes PRSS54 and CSNK2A2 and downstream from GINS3 and NDRG4 on the sense strand. PRSS54 is a trypsin-like serine protease which codes for the inactive serine protease 54 precursor. CSNK2A2 the casein kinase 2, alpha prime polypeptide contains a protein kinase domain and a catalytic domain. GINS3 is essential for the initiation of DNA replication and replisome progression in eukaryotes. NDRG4 a member of the N-myc downregulated gene family belonging to the alpha/beta hydrolase superfamily which encodes a cytoplasmic protein responsible for cell cycle progression and survival in primary astrocytes and may be involved in regulation of mitogenic signaling in vascular smooth muscle cells.

Homology

Paralogs

CCDC113 has one known paralog CCDC96 which has a query cover of 27% and a max identity value of 34%.
NameSpeciesSpecies Common NameNCBI Accession NumberLengthProtein Identity
CCDC113Homo sapiensHuman377aa100%
CCDC96Homo sapiensHuman555aa34%

Homologs

CCDC113 is highly conserved in all mammals and in organisms diverging back to Zebrafish, Danio rerio.
SpeciesSpecies common nameNCBI Accession Number Length Protein Identity
Homo sapiensHuman / 5304bp/377aa100%
Pan troglodytesChimpanzee / 5269bp/377aa99%
Mus musculusMouse / 1271bp/377aa78%
Felis catusCat / 1252bp/323aa69%
Xenopus tropicalisWestern Clawed Frog / 1395bp/441aa43%
Danio rerioZebrafish / 1154bp/358aa38%

Protein

The CCDC113 protein is composed of 377 amino acids which form a secondary structure composed primarily of alpha-helices. This protein contains a domain of unknown function DUF4201. There are many predicted post-translational modifications including phosphorylation, N-terminal acetylation, sumoylation, and N-glycosylation.

Function

The function of CCDC113 is currently unknown.

Expression

CCDC 113 is expressed at low levels in nearly all tissues of the body by RNA-seq including blood, lymph node, brain, heart, skeletal muscle, kidney, liver, colon, lung, thyroid, prostate, ovary, breast, adrenal gland, and adipocyte. The gene is also expressed in embryonic tissues and stem cells. There are high levels of expression in the cerebellum and in the testis and surrounding tissues.

Interactions

of CCDC113 include transcription factors ATF2, FOXD1, LCR-F1, C/EBPalpha, Max, AREB6, CBF-A, CBF, c-Myc, and HIF.
Interacting proteins found using two-hybrid screening techniques include GIT1; a G protein-coupled receptor kinase interacting ArfGAP, the cytoplasmic protein HAP1; Huntingtin-associated protein 1, IMMT, an inner mitochondrial membrane protein, and PFN2; Profilin 2- ubiquitous actin monomer binding protein.

Clinical significance

Studies have linked expression of CCDC113 in cancerous tissues to mutations present in the coding sequence. Missense mutations at location 86 from Arginine to Tryptophan and at R180C are related to adenocarcinomas of the colon. Two point mutations have also been linked to adenocarcinomas of the rectum, a missense mutation of R361Q and a base pair point mutation c972t. Serous carcinoma of the ovaries has been related to a missense mutation S6F.