Before 1959, both the IUPAP and IUPAC used oxygen to define the mole; the chemists defining the mole as the number of atoms of oxygen which had mass 16 g, the physicists using a similar definition but with the oxygen-16 isotope only. The two organizations agreed in 1959/60 to define the mole as follows.
The mole is the amount of substance of a system which contains as many elementary entities as there are atoms in 12 gram of carbon 12; its symbol is "mol".
This was adopted by the CIPM in 1967, and in 1971, it was adopted by the 14th CGPM. In 1961, the isotope carbon-12 was selected to replace oxygen as the standard relative to which the atomic weights of all the other elements are measured. In 1980, the CIPM clarified the above definition, defining that the carbon-12 atoms are unbound and in their ground state. In 2018, IUPAC specified the mole as exactly 6.022 140 76 × 1023 "elementary entities". The number of moles in 12 grams of carbon-12 became a matter of experimental determination.
Hoyle state
The Hoyle state is an excited, spinless, resonant state of carbon-12. It is produced via the triple-alpha process, and was predicted to exist by Fred Hoyle in 1954. The existence of the 7.7 MeV resonance Hoyle state is essential for the nucleosynthesis of carbon in helium-burning red giant stars, and predicts an amount of carbon production in a stellar environment which matches observations. The existence of the Hoyle state has been confirmed experimentally, but its precise properties are still being investigated. The Hoyle state is populated when a helium-4 nucleusfuses with a beryllium-8nucleus in a high-temperature environment with densely concentrated helium. This process must occur within 10−16 seconds as a consequence of the short half-life of 8Be. The Hoyle state also is a short-lived resonance with a half-life of seconds; it primarily decays back into its three constituent alpha particles, though 0.0413% of decays occur by internal conversion into the ground state of 12C. In 2011, an ab initio calculation of the low-lying states of carbon-12 found a resonance with all of the properties of the Hoyle state.
Isotopic purification
The isotopes of carbon can be separated in the form of carbon dioxide gas by cascaded chemical exchange reactions with amine carbamate.