Codocytes also known as target cells are red blood cells that have the appearance of a shooting target with a bullseye. In optical microscopy these cells appear to have a dark center surrounded by a white ring, followed by dark outer second ring containing a band of hemoglobin. However, in electron microscopy they appear very thin and bell shaped. Because of their thinness they are referred to as leptocytes and because of the wavy bowl shape they are referred to as Mexican hat cells or sombrero cells. On routine smear morphology, some people like to make a distinction between leptocytes and codocytes- suggesting that in leptocytes the central spot is not completely detached from the peripheral ring, i.e. the pallor is in a C shape rather than a full ring. These cells are characterized by a disproportional increase in the ratio of surface membranearea to volume. This is also described as a "relative membrane excess." It is due to either increased red cellsurface area, or else a decreased intracellular hemoglobin content. The increase in the surface area to volume ratio also gives the cell decreased osmotic fragility, as it allows it to take up more water for a given amount of osmotic stress. In vivo, the codocyte is a bell-shaped cell. It assumes a "target" configuration only when processed to obtain a blood film. In the film these cells appear thinner than normal, primarily due to their pallor.
Causes
Target cells may appear in association with the following conditions:
Post-splenectomy: A major function of the spleen is the clearance of opsonized, deformed, and damaged erythrocytes by splenic macrophages. If splenic macrophage function is abnormal or absent because of splenectomy, altered erythrocytes will not be removed from the circulation efficiently. Therefore, increased numbers of target cells may be observed.
In patients with obstructive liver disease, lecithin cholesterol acetyltransferase activity is depressed, which increases the cholesterol-to-phospholipid ratio and produces an absolute increase in the surface area of the red cell membrane. In contrast, membrane excess is only relative in patients with iron-deficiency anemia and thalassemia because of the reduced quantity of intracellular hemoglobin. When a cell membrane collapses it becomes static and stops pulsating. Target cell formation decreases the amount of oxygen that is circulated through the blood and unable to deliver it to all areas of the body.
Symptoms
Elevations in target cells are the result of a shift in the exchange equilibrium between the red blood cells and the cholesterol. Also the surface membrane to volume ratio is increased. Target cells are more resistant to osmotic lysis, which is mostly seen in dogs. Hypochromic cells in iron deficiency anemias also can show a target appearance. Target cells are abnormally resistant to saline.
