Double-disk diffusion test
A double-disk diffusion test is a kind of disk diffusion test
The double-disc synergy test utilizes two of these disks on the cultivated agar solution, either infused with a different antimicrobial solution.
This test was recommended the standard by the Clinical and Laboratory Standards Institute in 2004 for its use against MRSA. To test for clindamycin resistance certain strains of Staphylococcus aureus bacteria with natural resistance to erythromycin are chosen for cultivation on the gel. The two antimicrobial disks contain erythromycin and clindamycin and are placed about 20 mm apart. This is called a D-zone test, or D test. If a 'D' shape is formed around the clindamycin disk than the isolate is reported as resistant to clindamycin. This occurs due to erythromycin inducing the bacteria's ERM gene, and thus making it resistant to Clindamycin.
