SR 228 begins at an intersection with U.S. Route 90 in downtown Macclenny. At this intersection, the roadway continues as Baker County Road 228. It heads south-southeast through the city, passing Macclenny City Hall and the Emily Taber Public Library. Around the intersection with Jonathan Street, the road curves to the southeast. It crosses over Turkey Creek before leaving Macclenny. Approximately after leaving the city, it meets its eastern terminus, an interchange with Interstate 10. At this interchange, the roadway continues to the southeast as another instance of CR 228, this time known as Maxville–Macclenny Highway.
Eastern segment
Western terminus to Downtown
SR 228 resumes at an intersection with US 301 in the southwestern corner of Jacksonville. At this intersection, the roadway continues as Duval CR 228. US 301/SR 228 travel concurrently to the south-southwest for about, until SR 228 departs to the northeast on Normandy Boulevard. A short distance later, it meets the northern terminus of CR 217. Then, it travels through Cecil Center, passing the Cecil campus of Florida State College. It then has an interchange with SR 23. The highway passes by Herlong Recreational Airport, before meeting an interchange with I-295. SR 228 passes Gravely Hill Cemetery and John D. Liverman Park. While passing the park, the highway crosses over Cedar River. Then, it intersects SR 103. After that, it begins to curve to the east. At the intersection with Cassat Avenue, it takes on the Post Street name. At Hamilton Street, it heads northeast and curves back to the east past Edgewood Avenue. Then, it intersects SR 129. The two highways head concurrently to the south for about four blocks, to an intersection with US 17. At this intersection, SR 129 meets its southern terminus, and SR 228 heads concurrently with US 17 to the northeast. Less than later, the two highways have an interchange with I-10. I-10/US 17/SR 228 heads concurrently to the east. At an interchange with I-95, I-10 meets its eastern terminus, while I-95/US 17/SR 228 head to the northeast, crossing over McCoy Creek. At an interchange with Union Street, US 17/SR 228 splits off to the east, concurrent with US 23. The three highways enter downtown.
Downtown to eastern terminus
At Main Street, they intersect US 1. Here, US 23 meets its southern terminus and SR 228 turns onto US 1 south, while US 17 turns onto US 1 north. One block later, US 90 joins the concurrency. Four blocks farther to the south-southwest, SR 228 splits off onto West Monroe Avenue. One block to the east, at Ocean Street, it intersects the northbound lanes of US 1/US 90/SR 228. A short distance to the east-southeast, SR 228 meets the western terminus of the Hart Bridge Expressway, a freeway that connects downtown to the Southside neighborhood. Here, SR 228 joins with the expressway to the east-southeast. They pass VyStar Veterans Memorial Arena, EverBank Field, and WJCT, before US 1 Alternate joins the concurrency. The two highways cross over the St. Johns River on the Hart Bridge. Shortly after the bridge is an interchange with SR 10. Almost immediately, US 1 Alt. splits off onto Emerson Expressway. Then, SR 228 crosses over Little Pottsburg Creek. After that, it has an intersection with SR 109. The road continues to the east-southeast and curves to the southeast to meet its eastern terminus, an interchange with US 90.
There are two sections of SR 228 that is included as part of the NationalHighway System, a system of roadways important to the nation's economy, defense, and mobility :
From its western terminus to I-295
The entire section that is concurrent with the Hart Bridge Expressway
