Gainesville Regional Transit System
Gainesville Regional Transit System is the local area transit corporation that serves the Gainesville, Alachua County, Florida area, the University of Florida and Santa Fe College campuses. It presently serves 40 city routes, 10 campus routes, and five "Later Gator" routes. Paratransit service is also provided to anyone with a Gainesville address.
City routes
| No. | Description | Operates |
| 1 | Rosa Parks RTS Downtown Station to Butler Plaza RTS Transfer Station | Mon-Sun |
| 2 | Rosa Parks RTS Downtown Station to NE Walmart Supercenter | Mon-Sat |
| 3 | Rosa Parks RTS Downtown Station to N Main St. Post Office | Mon-Fri |
| 5 | Rosa Parks RTS Downtown Station to Oaks Mall | Mon-Sun |
| 6 | Rosa Parks RTS Downtown Station to North Walmart Supercenter | Mon-Sat |
| 7 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Fri |
| 8 | Shands to North Walmart Supercenter | Mon-Sun |
| 9 | Reitz Union to Hunters Run | Mon-Sun |
| 10 | Rosa Parks RTS Downtown Station to Santa Fe College | Mon-Sat |
| 11 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Sun |
| 12 | Reitz Union to Butler Plaza RTS Transfer Station | Mon-Sun |
| 13 | Beaty Towers at the University of Florida to Cottage Grove Apartments | Mon-Sun |
| 15 | Rosa Parks RTS Downtown Station to NW 13th St./NW 23rd Ave. | Mon-Sun |
| 16 | Beaty Towers at the University of Florida to Sugar Hill | Mon-Sun |
| 17 | Beaty Towers at the University of Florida to Rosa Parks RTS Downtown Station | Mon-Fri |
| 19 | Reitz Union to SW 23rd Terrace | Mon-Fri |
| 20 | Reitz Union to Oaks Mall | Mon-Sun |
| 21 | Reitz Union to Cabana Beach Apts | Mon-Fri |
| 23 | Oaks Mall to Santa Fe College | Mon-Fri |
| 24 | Rosa Parks RTS Downtown Station to Job Corps | Mon-Fri |
| 25 | UF Commuter Lot to Gainesville Regional Airport | Mon-Sun |
| 26 | Rosa Parks RTS Downtown Station to Gainesville Regional Airport | Mon-Fri |
| 27 | Rosa Parks RTS Downtown Station to NE Walmart Supercenter | Mon-Fri |
| 28 | The Hub to Butler Plaza RTS Transfer Station | Mon-Fri |
| 29 | Beaty Towers at the University of Florida to Kiwanis Park | Mon-Fri |
| 33 | Butler Plaza RTS Transfer Station to Midtown | Mon-Sun |
| 34 | The Hub to Lexington Crossing Apts | Mon-Fri |
| 35 | Reitz Union to SW 35th Place | Mon-Sun |
| 36 | The Hub to SW 34th St. Post Office | Mon-Fri |
| 37 | Reitz Union to Butler Plaza RTS Transfer Station | Mon-Sun |
| 38 | The Hub to Enclave Apts | Mon-Fri |
| 39 | Santa Fe College to Gainesville Regional Airport | Mon-Fri |
| 40 | The Hub to Hunters Crossing | Mon-Fri |
| 43 | Shands to Santa Fe College | Mon-Fri |
| 46 | Reitz Union to Rosa Parks RTS Downtown Station | Mon-Fri |
| 75 | Oaks Mall to Butler Plaza RTS Transfer Station | Mon-Sun |
| 76 | Santa Fe College to Haile Market Square | Mon-Fri |
| 77 | Santa Fe College to Cabana Beach Apts | Mon-Fri |
| 711 | Rosa Parks RTS Downtown Station to Eastwood Meadows | Mon-Fri after 8:00 p.m., all day Sat-Sun |
| 800x | Santa Fe College to Butler Plaza RTS Transfer Station | Mon-Fri |
Campus Service
10 RTS routes operate on the University of Florida campus. These include "park and ride" and circulator routes. UF and Santa Fe College students have prepaid unlimited access to RTS services through a transportation fee attached to each credit hour for undergraduates. UF faculty, staff, spouses and retirees have unlimited prepaid access through the RTS Employee Bus Pass Program, as do SF faculty, staff and students.| No. | Description | Operates |
| 117 | Reitz Union to Park-N Ride 2 | Mon-Fri |
| 118 | The Hub to Park-N Ride 1 | Mon-Fri |
| 119 | The Hub to Family Housing at the University of Florida | Mon-Fri |
| 120 | The Hub to University of Florida West Circulator | Mon-Fri |
| 121 | The Hub to UF Commuter Lot | Mon-Fri |
| 122 | University of Florida North/South Circulator | Mon-Fri |
| 125 | The Hub to Lakeside | Mon-Fri |
| 126 | Sorority Row to Lakeside | Mon-Fri |
| 127 | University of Florida East Circulator | Mon-Fri |
| 128 | Reitz Union to Lake Wauberg | Saturdays during spring and the first half of summer |
Later Gator
Later Gator is a late night service that provides safe, late night service for students between campus, downtown, the Oaks Mall and southwest Gainesville through five routes.| Route Letter | Description | Operates |
| A | Sorority Row to and from Downtown Station | 8:30pm – 3:20am Wednesday-Saturday |
| B | SW Gainesville to and from Downtown Station | 8:50pm – 3:13am Thursday-Saturday |
| C | Oaks Mall to and from Downtown Station | 8:30pm – 3:14am Thursday-Saturday |
| D | Cottage Grove Apts. to and from Downtown Station | 8:00pm – 2:30am Saturday Only |
| F | Butler Plaza to and from Downtown Station | 8:30pm – 3:18am Saturday Only |
Gator Aider
Gator Aider is a shuttle service provided to transport fans to and from University of Florida Gators home football games. Service is available at five park-n-ride locations around the Greater Gainesville area including the Town of Tioga, the Oaks Mall, Haile Plantation, the UF Hilton Conference Center and the City of Gainesville Downtown Parking Garage.Bus Tracking
In 2008, RTS equipped their fleet with a GPS capability called TransLoc. This enables riders to see if their bus is running on time. TransLoc Rider, a mobile phone app that incorporates the TransLoc GPS capability, is also available for riders.Fleet
Since 1974, Gainesville has had a diverse fleet of buses bought from Transit Authorities in state and manufacturers such as GMC, Orion Bus Industries, Blue Bird, Nova Bus, Flexible, Gillig Corporation. All RTS buses are equipped with bicycle racks and wheelchair lifts. While many were purchased new, others were acquired second-hand from other operators in the state, including 30 that were acquired from Lynx and 11 from Palm Tran. RTS acquired five new Gillig hybrid electric buses.Active Fleet
- Gillig Phantom
- Gillig Low Floor
- Nova Bus RTS