Golden rice
Golden rice is a variety of rice produced through genetic engineering to biosynthesize beta-carotene, a precursor of vitamin A, in the edible parts of rice. It is intended to produce a fortified food to be grown and consumed in areas with a shortage of dietary vitamin A, a deficiency which each year is estimated to kill 670,000 children under the age of 5 and cause an additional 500,000 cases of irreversible childhood blindness. Rice is a staple food crop for over half of the world's population, providing 30–72% of the energy intake for people in Asian countries, and becoming an effective crop for targeting vitamin deficiencies.
Golden rice differs from its parental strain by the addition of three beta-carotene biosynthesis genes. The parental strain can naturally produce beta-carotene in its leaves, where it is involved in photosynthesis. However, the plant does not normally produce the pigment in the endosperm, where photosynthesis does not occur. Golden rice has met significant opposition from environmental and anti-globalization activists. A study in the Philippines is aimed to evaluate the performance of golden rice, whether it can be planted, grown and harvested like other rice varieties, and whether golden rice poses risk to human health. There has been little research on how well the beta-carotene will hold up when stored for long periods between harvest seasons, or when cooked using traditional methods.
In 2005, Golden Rice 2 was announced, which produces up to 23 times as much beta-carotene as the original golden rice. To receive the USDA's Recommended Dietary Allowance, it is estimated that 144 g/day of the high-yielding strain would have to be eaten. Bioavailability of the carotene from golden rice has been confirmed and found to be an effective source of vitamin A for humans. Golden Rice was one of the seven winners of the 2015 Patents for Humanity Awards by the United States Patent and Trademark Office. In 2018 came the first approvals as food in Australia, New Zealand, Canada and the US.
History
The search for a golden rice started off as a Rockefeller Foundation initiative in 1982.biosynthesis pathway in golden rice. The enzymes expressed in the endosperm of golden rice, shown in red, catalyze the biosyntheis of beta-carotene from geranylgeranyl diphosphate. Beta-carotene is assumed to be converted to retinal and subsequently retinol in the animal gut
Peter Bramley discovered in the 1990s that a single phytoene desaturase gene can be used to produce lycopene from phytoene in GM tomato, rather than having to introduce multiple carotene desaturases that are normally used by higher plants. Lycopene is then cyclized to beta-carotene by the endogenous cyclase in golden rice.
The scientific details of the rice were first published in Science in 2000, the product of an eight-year project by Ingo Potrykus of the Swiss Federal Institute of Technology and Peter Beyer of the University of Freiburg. At the time of publication, golden rice was considered a significant breakthrough in biotechnology, as the researchers had engineered an entire biosynthetic pathway.
The first field trials of golden rice cultivars were conducted by Louisiana State University Agricultural Center in 2004. Additional trials have been conducted in the Philippines and Taiwan, and in Bangladesh. Field testing provides an accurate measurement of nutritional value and enables feeding tests to be performed. Preliminary results from field tests have shown field-grown golden rice produces 4 to 5 times more beta-carotene than golden rice grown under greenhouse conditions.
Crossbreeding
In several countries, golden rice has been bred with local rice cultivars. or crossbred with the American rice cultivar 'Cocodrie'. As of March 2016, golden rice has not yet been grown commercially, and backcrossing is still ongoing in current varieties to reduce yield drag.Golden Rice 2
In 2005, a team of researchers at Syngenta produced Golden Rice 2. They combined the phytoene synthase gene from maize with crt1 from the original golden rice. Golden rice 2 produces 23 times more carotenoids than golden rice, and preferentially accumulates beta-carotene.Approvals
In 2018, Canada and the United States approved golden rice for cultivation, with Health Canada and the US Food and Drug Administration declaring it safe for consumption. This followed a 2016 decision where the US Food and Drug Administration had ruled that the beta-carotene content in golden rice did not provide sufficient amounts of Vitamin A to make a nutritional claim. Health Canada declared that golden rice would not affect allergies, and that the nutrient contents of golden rice were the same as in common rice varieties, except for the intended high levels of provitamin A. In 2019, it was approved for direct use as human food and animal feed or for processing in the Philippines. This does not constitute approval for commercial propagation in the Philippines, which is a separate stage that remains to be completed.Genetics
Golden rice was created by transforming rice with two beta-carotene biosynthesis genes:- psy from daffodil
- crtI from the soil bacterium Erwinia uredovora
Vitamin A deficiency
The research that led to golden rice was conducted with the goal of helping children who suffer from vitamin A deficiency. In 2005, 190 million children and 19 million pregnant women, in 122 countries, were estimated to be affected by VAD. VAD is responsible for 1–2 million deaths, 500,000 cases of irreversible blindness and millions of cases of xerophthalmia annually. Children and pregnant women are at highest risk. Vitamin A is supplemented orally and by injection in areas where the diet is deficient in vitamin A., 43 countries had vitamin A supplementation programs for children under 5; in 10 of these countries, two high dose supplements are available per year, which, according to UNICEF, could effectively eliminate VAD. However, UNICEF and a number of NGOs involved in supplementation note more frequent low-dose supplementation is preferable.
Because many children in VAD-affected countries rely on rice as a staple food, genetic modification to make rice produce the vitamin A precursor beta-carotene was seen as a simple and less expensive alternative to ongoing vitamin supplements or an increase in the consumption of green vegetables or animal products. Initial analyses of the potential nutritional benefits of golden rice suggested consumption of golden rice would not eliminate the problems of vitamin A deficiency, but could complement other supplementation. Golden Rice 2 contains sufficient provitamin A to provide the entire dietary requirement via daily consumption of some 75g per day.
Since carotenes are hydrophobic, sufficient fat must be present in the diet for golden rice to alleviate vitamin A deficiency. Vitamin A deficiency is usually coupled to an unbalanced diet. Moreover, this claim referred to an early cultivar of golden rice; one bowl of the latest version provides 60% of RDA for healthy children. The RDA levels advocated in developed countries are far in excess of the amounts needed to prevent blindness.
Research
Clinical trials/food safety and nutrition research
In 2009, results of a clinical trial of golden rice with adult volunteers from the US were published in the American Journal of Clinical Nutrition. The trial concluded that "beta-carotene derived from golden rice is effectively converted to vitamin A in humans". A summary for the American Society for Nutrition suggested that "Golden Rice could probably supply 50% of the Recommended Dietary Allowance of vitamin A from a very modest amount – perhaps a cup – of rice, if consumed daily. This amount is well within the consumption habits of most young children and their mothers".It is well known that beta-carotene is found and consumed in many nutritious foods eaten around the world, including fruits and vegetables. Beta-carotene in food is a safe source of vitamin A. In August 2012, Tufts University and others published research on golden rice in the American Journal of Clinical Nutrition showing that the beta-carotene produced by golden rice is as effective as beta-carotene in oil at providing vitamin A to children. The study stated that "recruitment processes and protocol were approved". In 2015 the journal retracted the study, claiming that the researchers had acted unethically when providing Chinese children golden rice without their parents' consent.
The Food Allergy Resource and Research Program of the University of Nebraska undertook research in 2006 that showed the proteins from the new genes in Golden Rice 2 showed no allergenic properties.
Controversy
Critics of genetically engineered crops have raised various concerns. An early issue was that golden rice originally did not have sufficient provitamin A content. This problem was solved by the development of new strains of rice. The speed at which beta-carotene degrades once the rice is harvested, and how much remains after cooking are contested. However, a 2009 study concluded that beta-carotene from golden rice is effectively converted into vitamin A in humans and a 2012 study that fed 68 children ages 6 to 8 concluded that golden rice was as good as vitamin A supplements and better than the natural beta-carotene in spinach.Greenpeace opposes the use of any patented genetically modified organisms in agriculture and opposes the cultivation of golden rice, claiming it will open the door to more widespread use of GMOs. The International Rice Research Institute has emphasised the non-commercial nature of their project, stating that "None of the companies listed... are involved in carrying out the research and development activities of IRRI or its partners in Golden Rice, and none of them will receive any royalty or payment from the marketing or selling of golden rice varieties developed by IRRI."
Vandana Shiva, an Indian anti-GMO activist, argued the problem was not the plant per se, but potential problems with poverty and loss of biodiversity. Shiva claimed these problems could be amplified by the corporate control of agriculture. By focusing on a narrow problem, Shiva argued, golden rice proponents were obscuring the limited availability of diverse and nutritionally adequate food. Other groups argued that a varied diet containing foods rich in beta-carotene such as sweet potato, leaf vegetables and fruit would provide children with sufficient vitamin A. Keith West of Johns Hopkins Bloomberg School of Public Health has stated that foodstuffs containing vitamin A are often unavailable, only available at certain seasons, or too expensive for poor families in underdeveloped countries.
In 2008, WHO malnutrition expert Francesco Branca cited the lack of real-world studies and uncertainty about how many people will use golden rice, concluding "giving out supplements, fortifying existing foods with vitamin A, and teaching people to grow carrots or certain leafy vegetables are, for now, more promising ways to fight the problem". In 2013, author Michael Pollan, who had critiqued the product in 2001, unimpressed by the benefits, expressed support for the continuation of the research.
In 2012, controversy surrounded a study published in The American Journal of Clinical Nutrition. The study, involving feeding GM rice to 6 to 8 year old children in China, was later found to have violated human research rules of both Tufts University and the federal government. Subsequent reviews found no evidence of safety problems with the study, but found issues with insufficient consent forms, unapproved changes to study protocol, and lack of approval from a China-based ethics review board. Additionally, the GM rice used was brought into China illegally.
Support
The Bill and Melinda Gates Foundation supports the use of genetically modified organisms in agricultural development and supports the International Rice Research Institute in developing golden rice. In June 2016, 107 Nobel laureates signed a letter urging Greenpeace and its supporters to abandon their campaign against GMOs, and against golden rice in particular.In May 2018, the U.S. Food and Drug Administration approved the use of golden rice for human consumption, stating: "Based on the information IRRI has presented to FDA, we have no further questions concerning human or animal food derived from GR2E rice at this time." This marks the fourth national health organisation to approve the use of golden rice in 2018, joining Australia, Canada and New Zealand who issued their assessments earlier in the year.