Guatemala qualified two male archers based on its performance at the 2014 Pan American Championships. Later Guatemala qualified 1 woman based on its performance at the 2015 Copa Merengue.
Athletics
Guatemala qualified seven athletes. ;Key
Note–Ranks given for track events are for the entire round
Q = Qualified for the next round
q = Qualified for the next round as a fastest loser or, in field events, by position without achieving the qualifying target
SB = Seasonal best
DQ = Disqualified
N/A = Round not applicable for the event
;Men ;Women
Badminton
Guatemala has qualified a full team of eight athletes. ;Men ;Women ;Mixed
Beach volleyball
Guatemala has qualified a men's and women's pair for a total of four athletes.
Boxing
Guatemala qualified five male boxers. ;Men
Bowling
Canoeing
Sprint
Guatemala has received one wildcard in men's kayak. ;Men Qualification Legend: QF = Qualify to final; QS = Qualify to semifinal
Cycling
Guatemala has qualified nine cyclists. Some of the athletes will compete in multiple disciplines.
Road
Six cyclists competed for Guatemala in the road competitions. ;Men ;Women
Guatemala qualified 2 gymnasts. ;Men ;Individual Qualification ;Individual Finals Qualification Legend: Q = Qualified to apparatus final ;Women ;Individual Qualification ;Individual Finals Qualification Legend: Q = Qualified to apparatus final
Judo
Guatemala has qualified a team of three female judokas. ;Women
Modern pentathlon
Guatemala has qualified a team of 5 athletes.
Racquetball
Guatemala qualified a team of two men and two women for a total of four athletes. ;Singles and Doubles ;Team
Roller sports
Guatemala has qualified two athletes in the speed competitions.
Speed
Rowing
Guatemala has qualified 4 boats. Qualification Legend: FA=Final A ; FB=Final B ; R=Repechage
Sailing
Guatemala qualified 4 boats.
Squash
Guatemala has qualified a full team of six athletes. ;Singles and Doubles ;Team
Shooting
Guatemala qualified 21 shooters. ;Men ;Women
Swimming
Guatemala qualified eight swimmers. ;Key
Note – Ranks given are for the entire round
FA = Qualified for the A Final
FB = Qualified for the B Final
DNS = Did not start
;Men
Note: Peruvian swimmer Mauricio Fiol was suspended by his team and pulled from the A final of the 100 meter butterfly for testing positive for stanozolol. Therefore, Luis Martinez was able to swim in the A final.
;Women
Synchronized swimming
Guatemala qualified a duet. The team originally did not qualify, but after Puerto Rico declined its quota earned at the 2014 Central American and Caribbean Games in Veracruz, Mexico, the country was allowed to enter a duet as the first reserve. The duet ended up finishing in last place.
Table tennis
Guatemala has qualified a men's and women's team. ;Men ;Women
Guatemala received a reallocated quota spot in the water skiing competitions. ;Men
Weightlifting
Guatemala qualified a team of 4 athletes. Guatemala's fourth athlete tested positive for doping on May 23 (but results were determined only on July 9 when she was in Toronto and was disqualified before the games started. She was scheduled to compete in the women's +75 kg category.
Wrestling
Guatemala qualified one male athlete. ;Men's freestyle
