Histamine H3 receptors are expressed in the central nervous system and to a lesser extent the peripheral nervous system, where they act as autoreceptors in presynaptic histaminergic neurons, and also control histamine turnover by feedback inhibition of histamine synthesis and release. The H3 receptor has also been shown to presynaptically inhibit the release of a number of other neurotransmitters including, but probably not limited to dopamine, GABA, acetylcholine, noradrenaline, histamine and serotonin. The gene sequence for H3 receptors expresses only about 22% and 20% homology with both H1 and H2 receptors respectively. There is much interest in the histamine H3 receptor as a potential therapeutic target because of its involvement in the neuronal mechanism behind many cognitive H3R-disorders and especially its location in the central nervous system.
Like all histamine receptors, the H3 receptor is a G-protein coupled receptor. The H3 receptor is coupled to the GiG-protein, so it leads to inhibition of the formation of cAMP. Also, the β and γ subunits interact with N-type voltage gated calcium channels, to reduceaction potential mediated influx of calcium and hence reduce neurotransmitter release. H3 receptors function as presynaptic autoreceptors on histamine-containing neurons. The diverse expression of H3 receptors throughout the cortex and subcortex indicates its ability to modulate the release of a large number of neurotransmitters. H3 receptors are thought to play a part in the control of satiety.
Isoforms
There are at least six H3 receptor isoforms in the human, and more than 20 discovered so far. In rats there have been six H3receptor subtypes identified so far. Mice also have three reported isoforms. These subtypes all have subtle difference in their pharmacology but the exact physiological role of these isoforms is still unclear.
Pharmacology
Agonists
There are currently no therapeutic products acting as selective agonists for H3 receptors, although there are several compounds used as research tools which are reasonably selective agonists. Some examples are:
-α-methylhistamine
Cipralisant
Imbutamine
Immepip
Imetit
Immethridine
Methimepip
Proxyfan
Antagonists
These include:
A-304121
A-349,821
ABT-239
Betahistine
Burimamide
Ciproxifan
Clobenpropit
Conessine
Failproxifan
Impentamine
Iodophenpropit
Irdabisant
Pitolisant
Thioperamide
VUF-5681
Therapeutic potential
The H3-receptor is a promising potential therapeutical target for many disorders that are caused by a histaminergic H3R dysfunction, because it is linked to the central nervous system and its regulation of other neurotransmitters. Examples of such disorders are: sleep disorders, Tourette syndrome, Parkinson, OCD, ADHD, ASS and addictions. This receptor has been proposed as a target for treating sleep disorders. The receptor has also been proposed as a target for treating neuropathic pain. Because of its ability to modulate other neurotransmitters, H3receptor ligands are being investigated for the treatment of numerous neurological conditions, including obesity, movement disorders, schizophrenia and ADHD and research is underway to determine whether H3 receptor ligands could be useful in modulating wakefulness. There is also evidence that the H3-receptor plays an important role in Tourette syndrome. Mouse-models and other research demonstrated that reducing histamine concentration in the H3R causes tics, but adding histamine in the striatum decreases the symptoms. The interaction between histamine and dopamine as well as other neurotransmitters is an important underlying mechanism behind the disorder.
History
1983 The H3 receptor is pharmacologically identified.
1988 H3 receptor found to mediate inhibition of serotonin release in rat brain cortex.