Holtfreter's solution
Holtfreter's solution is a balanced salt solution that was developed by the developmental biologist Johannes Holtfreter for studying amphibian embryos and to reduce bacterial infections. As a specialised aqueous solution, it finds use in aquaria to prevent infections for early stage amphibians, where it is typically mixed with soft tap water. Amphibians such as axolotls prefer a hard watersolution.Composition
of the component salts are as follows:
| NaCl | 0.059 M |
| KCl | 0.00067 M |
| CaCl2 | 0.00076 M |
| NaHCO3 | 0.0024 M |
