These states were to have a force of 200 galleys, 100 other ships, 50,000 infantry, 4,500 cavalry and adequate artillery ready by 1 April each year. Don Juan de Austria, illegitimate half-brother of King Philip II of Spain, was designated supreme commander. The League kept membership open for the Holy Roman Empire, France and Portugal, but none of them joined. The Empire preferred to maintain its truce with Istanbul, while France had an active anti-Spanish alliance with the Ottomans. Portugal had no forces to spare, owing to its heavy engagement in its own Moroccan campaign, its ongoing maritime confrontations with the Ottomans in the Red Sea and the Indian Ocean, and colonial conflicts with the Malaccan and Johorean Sultanates. The League initially assembled a fleet to aid the Venetian defenders of Cyprus which was invaded by Ottoman forces under the command of Lala Mustafa in July 1570, but was too late to prevent the island's capture by the Ottomans. On 7 October 1571, the League won a decisive victory over the Ottoman fleet at the Battle of Lepanto in the Gulf of Patras. The fleet of the Holy League in this engagement consisted of 212 warships with 1,815 guns and manned by 28,500 infantry. The majority of warships were Venetian, the next largest contingent were Spanish, and Genoese, with additional warships from the Papal States, the Order of Saint Stephen from the Grand Duchy of Tuscany, the Duchy of Savoy and the Knights of Malta, and some privately owned galleys in Spanish service. The victory at Lepanto confirmed the de facto division of the Mediterranean, with the eastern half under firm Ottoman control and the western under the Habsburgs and their Italian allies. The following year, as the allied Christian fleet resumed operations, it faced a renewed Ottoman navy of 200 vessels under Kılıç Ali Pasha, but the Ottoman commander actively avoided engaging the allied fleet and headed for the safety of the fortress of Modon. The arrival of the Spanish squadron of 55 ships evened the numbers on both sides and opened the opportunity for a decisive blow, but friction among the Christian leaders and the reluctance of Don John squandered the opportunity. Pius V died on 1 May 1572. The diverging interests of the League members began to show, and the alliance began to unravel. In 1573, the Holy League fleet failed to sail altogether; instead, Don John attacked and took Tunis, only for it to be retaken by the Ottomans in 1574. Venice, fearing the loss of her Dalmatian possessions and a possible invasion of Friuli, and eager to cut her losses and resume the trade with the Ottoman Empire, initiated unilateral negotiations with the Porte. The Holy League was disbanded with the peace treaty of 7 March 1573, which concluded the War of Cyprus.
