Ott's early career was in finance. He gained a degree at Harvard Business School, and worked for several Chicago area banks. During this period, he developed an interest in time-lapse photography. He made his first time-lapse film in 1927, while he was still in high-school, but did not develop the automated mechanical camera rig which he used in his later films, until 1937. His early amateur films led him to a career in cinematography. In addition to making films, he performed research about the effects of natural lighting on plants, animals, and humans. Initially, Ott's interest in time-lapse movie photography, mostly of plants, was just a hobby. Starting in the 1930s, Ott bought and built more and more time-lapse equipment, eventually building a large greenhouse full of plants, cameras, and self-built automated electric moving camera systems for moving the cameras to follow the growth of plants as they developed. He time-lapsed his entire greenhouse of plants and cameras as they all worked and grew. His work caught the attention of organizations who employed him to make time-lapse segments for films they were producing, including the educational filmSecrets of Life, for the Walt Disney Company in 1956, and The Story of Wheat, for the Santa Fe Railroad. From the late-1940s to the early-1950s he hosted a Chicago-area television program called How Does Your Garden Grow, which featured gardening tips, botanical information, and short time-lapse films. Episodes of this program can be viewed on the YouTube page of the WinnetkaHistorical Society. Ott developed a theory in the later years of his life that movement of plants could be manipulated by varying the amount of water that plants were given, and varying the color temperature of the lights in the studio, with some colors causing the plants to flower and other colors causing the plants to bear fruit. His 1958 memoir My Ivory Cellar details not only his personal history as a filmmaker, but also outlines these ideas. Ott's experiments with different colored lighting systems and their effects on the health of plants led to experiments with colored lights on the health of animals, humans, and individual cells, using time-lapse micro-photography. Ott's experiments led him to believe that only a full spectrum of natural light could promote full health in plants, animals, and humans. He wrote a 1973 book entitled Health and Light advancing his theories. Such topics were also addressed in his 1974 filmExploring the Spectrum.
Ott believed that the proprietary lighting technology he developed was the closest replication to the sun's natural wavelengths. He developed several consumer products based on this technology, including altered lightbulbs and a special pair of polarized sunglasses which he believed would enhance the physical and mental fortitude of anyone wearing them. In 1989, entrepreneur Fred Mendelsohn started Environmental Lighting Technologies and worked with Ott to develop the technology into award-winning products for businesses and homes. OttLite describes its "508 technology" as providing a balance of brightness and contrast that reduces glare and eyestrain and produces very low heat. The products, called "High Definition Natural Lighting", are marketed online and at lighting, crafting, and hardware stores.