Katharine Bartlett was born on November 30, 1907 in Denver, Colorado to Louise Erina and George Frederick Bartlett. Unable to afford her first choice of Smith College, Bartlett obtained her master's degree in physical anthropology from the University of Denver, studying under Etienne Bernardeau Renaud. In 1930, she took a summer position to assist with the Hopi Craftsman Exhibition of the Museum of Northern Arizona. At the invitation of Harold Sellers Colton, who at the time was the preeminent expert in Southwestern US archaeological and ethnological research, Bartlett stayed on in Arizona to organize the two-year-old MNA, which Colton and his wife had founded. Bartlett organized, cataloged and preserved the museum's anthropology collection serving from 1930 to 1952 as the museum curator. In 1931, she and Colton made an archaeological survey of the Navajo Reservation covering 250 miles of the reservation which lies in the Little Colorado River basin. They plotted 260 archaeological sites in their survey and her work on the artifacts from the gravel beds near Tolchaco was some of the earliest work on the Paleo-Indian groups in the area. In 1935, she was honored as one of the first four women elected as Fellows of the Society for American Archaeology. Her work on Paleo-Indians presented at the 1946 Pecos Conference correctly showed that previous work by Frank Roberts indicating a break between Folsom tradition and later cultures was erroneous. In 1952, when Gene Field Foster, Bartlett's housemate, began recording archaeological sites in the area where the Glen Canyon Dam was being built, she invited Bartlett and the MNA to participate. Bartlett established the catalog system for the archaeological collection of the Glen Canyon Project which was the largest project sponsored through the Interagency Archeological Salvage Program in the Santa Fe Regional Office of the National Park Service. The surveying in Glen Canyon continued for more than five years. Beginning in 1928, Bartlett published numerous articles on the Native people and cultures of Arizona. Her scientific papers included such topics as ancient mines, artifacts, foods, history, prehistoric tools, as well as crafts of the Hopi, Navajo, and other Arizona tribes. Her article Pueblo Milling Stones of the Flagstaff Region and Their Relation to Others in the Southwest: A Study in Progressive Efficiency "has become a standard reference on groundstone food-processing technology", according to the Society for American Archaeology. From 1953 until her retirement in 1981, she served as the Librarian of the MNA and collected thousands of volumes to create a comprehensive research facility in northern Arizona. Bartlett was a charter member of the Arizona Academy of Science and the Arizona Association of University Women. She was a Fellow of the American Association for the Advancement of Science, of the American Anthropological Association, as well as the first Fellow of the MNA. She was honored by an exhibit which appeared at the Smithsonian in 1986, entitled “Daughter of the Desert” and a 1991 recipient of the Sharlot Hall Award for her contributions to Arizona history. Bartlet retired in 1981 to care for her housemate Foster, who died in 1983. Into the 1990s, she continued working at the MNA as a volunteer. Bartlett died on May 22, 2001 in Sedona, Arizona and was posthumously inducted into the Arizona Women's Hall of Fame in 2008.