| Route | Terminus A | Terminus B | For events at |
| 3a | St Kilda Junction | Dandenong and Derby Roads, Caulfield East | Caulfield Racecourse |
| 30a | Melbourne Central railway station | Central Pier | Docklands Stadium |
| 57a | Flinders Street railway station | Sandown and Epsom Roads, Flemington | Melbourne Showgrounds |
| 59a | Flinders Street railway station | Moonee Ponds Junction | Moonee Valley Racecourse |
| 70a | Rod Laver Arena | Flinders Street West | Rod Laver Arena |
| 70a | Melbourne Cricket Ground | Flinders Street West | Melbourne Cricket Ground, Melbourne Park Multi-Purpose Venue |
| 70a | Melbourne Rectangular Stadium | Flinders Street West | Melbourne Rectangular Stadium |
| 75a | Simpsons Street and Wellington Parade, East Melbourne | Flinders Street West | Melbourne Cricket Ground |
| 75a | Flinders Street railway station | Harbour Town Shopping Centre | Victoria Harbour |
| 86a | Russell & Bourke Streets, City | Central Pier | Docklands Stadium |
| 86a | Russell & Bourke Streets, City | Harbour Town Shopping Centre | Victoria Harbour |
Route | Terminus A | Terminus B | First service | Last service | Notes |
4 | Carnegie | City | 24 June 1934 | 31 October 1970 | Replaced by route 67 following route number revision. |
4d | East Malvern | City | 24 June 1934 | 31 October 1970 | Replaced by route 3 following route number revision. |
7 | Camberwell | City | 21 November 1929 | 31 October 1970 | Replaced by route 72 following route number revision. |
8 | Moreland | Toorak | 17 October 2004 | 30 April 2017 | Merged into route 58. Change associated with the construction of Melbourne Metro Rail Tunnel. |
9 | Northcote | City | 1 November 1970 | 14 March 1993 | via Brunswick Street |
10 | West Preston | South Melbourne and St Kilda Beach | 25 June 1937 | 29 April 1995 | Discontinued following elimination of shared depot routes. |
13 | West Preston | City | August 1968 | 26 June 1989 | via La Trobe Street; Discontinued following timetable change at East Preston Depot. |
14 | East Preston | City | 1 November 1970 | 26 June 1989 | via La Trobe Street; Discontinued following timetable change at East Preston Depot. |
15 | Moreland | St Kilda Beach | 26 April 1936 | 1 April 1995 | Split into routes 16 and 22 following elimination of shared depot routes. |
22 | Moreland | Arts Centre | 1 November 1970 | 16 October 2004 | Merged into route 8. |
23 | Mont Albert | City | 25 September 1972 | 7 September 2001 | via La Trobe Street |
24 | North Balwyn | City | 25 September 1972 | 26 July 2014 | via La Trobe Street; Discontinued based on the desire to end peak-hour only routes. |
25 | Moreland | Domain Road | 2 April 1995 | 6 October 2000 | Extension of Route 22 that ran only during morning peaks. |
27 | Hawthorn | City | 6 June 1934 | 12 February 1965 | Discontinued following when Route 74/75 diverted via Bridge Road to Flinders Street when Hawthorn Depot closed. |
31 | St Vincent's Plaza | Victoria Harbour | 25 September 1972 | 26 July 2014 | Discontinued following network-wide timetable revision. |
32 | Camberwell | City | 25 September 1972 | 17 November 1986 | Discontinued once through-routing on William Street routes ceased. |
33 | Domain Road | City | 17 November 1986 | 1995 | Peak hour variant that gradually fell out of use after the closure of South Melbourne Depot. |
34 | East Melbourne | City | 1995 | 19 September 2003 | Route 30 became full-time |
35 | Malvern | City | 25 September 1972 | 17 November 1986 | Discontinued once through-routing on William Street routes ceased. |
36 | Glen Iris | City | 25 September 1972 | 17 November 1986 | Discontinued once through-routing on William Street routes ceased. |
37 | Carnegie | City | 25 September 1972 | 17 November 1986 | Discontinued once through-routing on William Street routes ceased. |
38 | Toorak | City | 25 September 1972 | 17 November 1986 | Discontinued once through-routing on William Street routes ceased. |
42 | Mont Albert | City | 6 June 1934 | 18 December 1993 | Merged with route 111 into route 109. |
55 | West Coburg | Domain Interchange | 30 April 1995 | 30 April 2017 | Merged into route 58. Change associated with the construction of Melbourne Metro Rail Tunnel. |
56 | West Brunswick | Domain Interchange | 3 February 1946 | 29 April 1995 | Discontinued to fix route number anomaly with route 55. |
68 | West Coburg | City | 1 November 1970 | 7 August 2005 | Route 55 began seven-day operation. |
69 | Kew | St Kilda Beach | 24 June 1934 | 15 October 2004 | Merged into route 16. |
74 | Burwood | City | 9 December 1934 | 18 July 1978 | Replaced by Route 75 following East Burwood extension. |
77 | Prahran | Batman Avenue | 9 December 1934 | 1 November 1986 | Discontinued due to low patronage/cost-saving. |
79 | North Richmond | St Kilda Beach | 9 December 1934 | 26 July 2014 | Route 78 became full-time. |
88 | East Preston | City | 26 June 1955 | 17 May 1983 | Replaced by route 86 following Bundoora extension. |
95 | Exhibition | Etihad Stadium | 26 June 1955 | 26 July 2014 | Discontinued based on the desire to end peak-hour only routes. |
111 | Exhibition | Port Melbourne | 20 November 1987 | 18 December 1993 | Merged with route 111 into route 109. |
112 | West Preston | South Melbourne and St Kilda Beach | April 2000 | 26 July 2014 | Split into routes 11 and 12 following network-wide timetable revision. |