List of Ohio railroads
The following railroads operate in the U.S. state of Ohio.
Common freight carriers
- Akron Barberton Cluster Railway
- Ann Arbor Railroad, Now owned by Watco Inc
- Ashland Railway
- Ashtabula, Carson and Jefferson Railroad
- Camp Chase Industrial Railroad
- Canadian National Railway through subsidiaries Bessemer and Lake Erie Railroad and Grand Trunk Western Railroad
- Central Railroad of Indiana
- Chicago, Fort Wayne and Eastern Railroad
- *Operates Delphos Terminal
- Cincinnati East Terminal Railway
- Cleveland Commercial Railroad
- Cleveland Harbor Belt Railroad
- Cleveland Works Railway
- Columbus and Ohio River Rail Road
- CSX Transportation including subsidiary Three Rivers Railway
- Flats Industrial Railroad
- Grand River Railway
- Indiana Eastern Railroad
- Indiana Northeastern Railroad
- Indiana and Ohio Railway
- *Operates South Charleston Railroad, West Central Ohio Port Authority
- Kanawha River Railroad
- Lake Terminal Railroad
- Lorain Northern Railroad
- Mahoning Valley Railway
- Napoleon Defiance and Western, Owned by Pioneer Railcorp
- Newburgh and South Shore Railroad
- Norfolk Southern Railway including subsidiary Cincinnati, New Orleans and Texas Pacific Railway
- Northern Ohio and Western Railway
- Ohi-Rail Corporation
- Ohio Central Railroad
- Ohio South Central Railroad
- Ohio Southern Railroad
- Ohio Terminal Railway
- Republic N&T Railroad
- R.J. Corman Railroad/Cleveland Line
- R.J. Corman Railroad/Western Ohio Lines
- RSL Railroad
- Warren and Trumbull Railroad
- Wheeling and Lake Erie Railway
- Youngstown and Austintown Railroad
- Youngstown Belt Railroad
- Youngstown and Southeastern Railroad
- *Operates Eastern States Railroad and Ohio and Pennsylvania Railroad
Private freight carriers
Passenger carriers
Defunct railroads
| Name | Mark | System | From | To | Successor | Notes |
| Addyston and Ohio River Railroad | 1889 | 1914 | N/A | - | ||
| Adena Railroad | W&LE | 1901 | 1916 | Wheeling and Lake Erie Railway | - | |
| Adena, Cadiz and New Athens Railway | W&LE | 1914 | 1917 | Wheeling and Lake Erie Railway | - | |
| Akron and Barberton Belt Railroad | A&BB, ABB | ACY/ B&O/ ERIE/ PRR | 1902 | 1994 | Akron Barberton Cluster Railway | - |
| Akron Branch of the Cleveland and Pittsburgh Railroad | PRR | 1851 | 1853 | Cleveland, Zanesville and Cincinnati Railroad | - | |
| Akron, Canton and Youngstown Railroad | ACY | ACY | 1944 | 1982 | Norfolk and Western Railway | - |
| Akron, Canton and Youngstown Railway | ACY | ACY | 1907 | 1944 | Akron, Canton and Youngstown Railroad | - |
| Akron and Chicago Junction Railroad | B&O | 1890 | 1915 | Baltimore and Ohio Railroad | - | |
| Akron and New Castle Railroad | ACY | 1892 | 1907 | Akron, Canton and Youngstown Railway | - | |
| Akron and Pittsburgh Railroad | ACY | 1895 | 1899 | Northern Ohio Railway | - | |
| Akron Terminal Railway | ACY/ B&O/ ERIE/ PRR | 1901 | 1901 | Barberton, Akron and Eastern Railway | - | |
| Akron Transfer Railroad | ACY | 1891 | 1902 | Richland and Mahoning Railway | - | |
| Akron Union Passenger Depot Company | B&O/ PRR | 1891 | 1975 | N/A | - | |
| Alliance and Lake Erie Railroad | NYC | 1878 | 1882 | Cleveland, Youngstown and Pittsburgh Railway | - | |
| Alliance, Niles and Ashtabula Railroad | PRR | 1881 | 1887 | Ashtabula, Niles and Youngstown Railroad | - | |
| Alliance and Northern Railroad | NYC | 1891 | 1902 | Lake Erie, Alliance and Wheeling Railroad | - | |
| American Central Railway | NKP | 1859 | 1864 | Cleveland, Fort Wayne and Chicago Railroad | - | |
| American Midland Railway | 1889 | 1890 | Findlay and Western Railroad | - | ||
| Ann Arbor Railroad | AA | AA | 1895 | 1976 | Consolidated Rail Corporation | - |
| Ashland and Western Railroad | ERIE/ PRR | 1904 | 1906 | Ashland and Western Railway | - | |
| Ashland and Western Railway | ERIE/ PRR | 1906 | 1913 | Lorain, Ashland and Southern Railroad | - | |
| Ashland and Wooster Railway | ERIE/ PRR | 1897 | 1903 | Lake and River Railway | - | |
| Ashtabula and New Lisbon Railroad | ERIE, PRR | 1853 | 1870 | Ashtabula, Youngstown and Pittsburgh Railroad, Niles and New Lisbon Railway | - | |
| Ashtabula, Niles and Youngstown Railroad | PRR | 1887 | 1887 | Pittsburgh, Youngstown and Ashtabula Railroad | - | |
| Ashtabula and Pittsburgh Railway | PRR | 1878 | 1887 | Ashtabula, Niles and Youngstown Railroad | - | |
| Ashtabula, Youngstown and Pittsburgh Railroad | PRR | 1870 | 1878 | Ashtabula and Pittsburgh Railway | - | |
| Athens, Amesville and Chauncey Railway | C&O | 1903 | 1909 | Hocking Valley Railway | - | |
| Atlantic and Great Western Railroad | ERIE | 1871 | 1880 | New York, Pennsylvania and Ohio Railroad | - | |
| Atlantic and Great Western Railroad | ERIE | 1853 | 1865 | Atlantic and Great Western Railway | - | |
| Atlantic and Great Western Railway | ERIE | 1865 | 1871 | Atlantic and Great Western Railroad | - | |
| Atlantic and Lake Erie Railway | NYC | 1869 | 1876 | Ohio Central Railway | - | |
| Baltimore and Ohio Railroad | B&O, BO | B&O | 1866 | 1987 | Chesapeake and Ohio Railway | - |
| Baltimore and Ohio and Chicago Railroad | B&O | 1876 | 1949 | Baltimore and Ohio Railroad | - | |
| Baltimore and Ohio Southwestern Railroad | B&O | 1899 | 1949 | Baltimore and Ohio Railroad | - | |
| Baltimore and Ohio Southwestern Railroad | B&O | 1889 | 1893 | Baltimore and Ohio Southwestern Railway | - | |
| Baltimore and Ohio Southwestern Railway | B&O | 1893 | 1899 | Cincinnati, Portsmouth and Parkersburg Railway | - | |
| Baltimore and Ohio Southwestern Terminal Company | B&O | 1891 | 1899 | Baltimore and Ohio Southwestern Railroad | - | |
| Baltimore and Ohio, Toledo and Michigan Railroad | PRR | 1870 | 1871 | Toledo, Tiffin and Eastern Railroad | - | |
| Baltimore, Pittsburgh and Chicago Railway | B&O | 1872 | 1876 | Baltimore and Ohio and Chicago Railroad | - | |
| Baltimore, Pittsburgh and Continental Railroad | ERIE | 1871 | 1873 | Chicago and Atlantic Railway | - | |
| Baltimore Short Line Railway | B&O | 1870 | 1888 | Cincinnati, Washington and Baltimore Railroad | - | |
| Barberton, Akron and Eastern Railway | ACY/ B&O/ ERIE/ PRR | 1901 | 1902 | Barberton, Akron and Eastern Belt Line Railway | - | |
| Barberton, Akron and Eastern Belt Line Railway | ACY/ B&O/ ERIE/ PRR | 1902 | 1902 | Akron and Barberton Belt Railroad | - | |
| Barberton Belt Line Railroad | ACY/ B&O/ ERIE/ PRR | 1891 | 1902 | Akron and Barberton Belt Railroad | - | |
| Barberton and Southern Railroad | ACY/ B&O/ ERIE/ PRR | 1899 | 1905 | Akron and Barberton Belt Railroad | - | |
| Bay Terminal Railroad | 1895 | 1955 | N/A | - | ||
| Bellaire, Beaver Valley and Shawnee Railway | B&O | 1879 | 1882 | Wheeling and Cincinnati Mineral Railway | - | |
| Bellaire and St. Clairsville Railway | B&O | 1885 | 1888 | Central Ohio Railroad | - | |
| Bellaire and St. Clairsville Narrow Gauge Railway | B&O | 1876 | 1880 | St. Clairsville Railway | - | |
| Bellaire and South Western Railway | PRR | 1875 | 1882 | Bellaire, Zanesville and Cincinnati Railway | - | |
| Bellaire and Valley Junction Railway | W&LE | 1890 | 1901 | Adena Railroad | - | |
| Bellaire, Zanesville and Cincinnati Railway | PRR | 1882 | 1902 | Ohio River and Western Railway | - | |
| Bellefontaine Railway | NYC | 1864 | 1868 | Cleveland, Columbus, Cincinnati and Indianapolis Railway | - | |
| Bellefontaine and Indiana Railroad | NYC | 1848 | 1864 | Bellefontaine Railway | - | |
| Belmont Central Railroad | B&O | 1907 | 1914 | Cleveland, Lorain and Wheeling Railway | - | |
| Belpre and Cincinnati Railroad | B&O | 1845 | 1851 | Marietta and Cincinnati Railroad | - | |
| Bowling Green Railroad | B&O | 1874 | 1943 | Toledo and Cincinnati Railroad | - | |
| Butler and Detroit Railroad | WAB | 1880 | 1881 | Detroit, Butler and St. Louis Railroad | - | |
| Canal Belt Railroad | ERIE | - | ||||
| Canton and Waynesburgh Railroad | W&LE | 1884 | 1888 | Waynesburg and Canton Railroad | - | |
| Canton and Wooster Railroad | - | |||||
| Carroll County Railroad | W&LE | 1850 | 1866 | Carrollton and Oneida Railroad | - | |
| Carrollton and Oneida Railroad | W&LE | 1866 | 1873 | Ohio and Toledo Railroad | - | |
| Celina, Van Wert and State Line Extension of the Columbus and North-Western Railway | NYC | 1878 | 1881 | Cincinnati, Van Wert and Michigan Railroad | - | |
| Central Columbiana and Pennsylvania Railway | CQPA | 2001 | 2004 | Ohio and Pennsylvania Railroad | - | |
| Central Ohio Railroad | B&O | 1847 | 1915 | Baltimore and Ohio Railroad | - | |
| Central Union Depot and Railway Company of Cincinnati | B&O/NYC | 1884 | 1935 | N/A | - | |
| Central Valley Railway | W&LE | 1901 | 1916 | Wheeling and Lake Erie Railroad | - | |
| Chagrin Falls and Lake Erie Railroad | W&LE | 1901 | 1916 | Wheeling and Lake Erie Railway | - | |
| Chagrin Falls and Southern Railroad | W&LE | 1880 | 1890 | Cleveland, Chagrin Falls and Northern Railroad | - | |
| Chesapeake and Hocking Railway | C&O | 1926 | 1930 | Chesapeake and Ohio Railway | - | |
| Chesapeake and Ohio Railway | C&O, CO | C&O | 1889 | 1987 | CSX Transportation | - |
| Chesapeake and Ohio Northern Railway | C&O | 1914 | 1930 | Chesapeake and Ohio Railway | - | |
| Chesapeake and Ohio Railway of Indiana | C&O | 1910 | 1934 | Chesapeake and Ohio Railway | - | |
| Chicago and Atlantic Railway | ERIE | 1873 | 1890 | Chicago and Erie Railroad | - | |
| Chicago, Cincinnati and Louisville Railroad | C&O | 1903 | 1910 | Chesapeake and Ohio Railway of Indiana | - | |
| Chicago and Erie Railroad | ERIE | 1890 | 1941 | Erie Railroad | - | |
| Chicago and Canada Southern Railway | NYC | 1871 | 1888 | Detroit and Chicago Railroad | - | |
| Chicago, St. Louis and Pittsburgh Railroad | PRR | 1883 | 1890 | Pittsburgh, Cincinnati, Chicago and St. Louis Railway | - | |
| Chicago, Zanesville and Atlantic Railway | B&O | 1885 | 1886 | Zanesville and Ohio River Railway | - | |
| Cincinnati and Baltimore Railway | B&O | 1868 | 1894 | Cincinnati, Washington and Baltimore Railroad | - | |
| Cincinnati, Batavia and Williamsburg Railroad | N&W | 1876 | 1876 | Cincinnati and Eastern Railway | - | |
| Cincinnati, Columbus and Hocking Valley Railway | DT&I | 1881 | 1886 | Ohio Southern Railroad | - | |
| Cincinnati Connecting Belt Railroad | N&W | 1899 | 1901 | Cincinnati, Portsmouth and Virginia Railroad | - | |
| Cincinnati and Dayton Railway | B&O | 1887 | 1943 | Toledo and Cincinnati Railroad | - | |
| Cincinnati, Dayton and Chicago Railroad | B&O | 1891 | 1895 | Cincinnati, Hamilton and Dayton Railway | - | |
| Cincinnati, Dayton and Ironton Railroad | B&O | 1891 | 1895 | Cincinnati, Hamilton and Dayton Railway | - | |
| Cincinnati and Eastern Railway | N&W | 1876 | 1887 | Ohio and North Western Railroad | - | |
| Cincinnati, Findlay and Fort Wayne Railway | 1903 | 1919 | N/A | - | ||
| Cincinnati, Georgetown and Portsmouth Railroad | CG&P | 1873 | - | |||
| Cincinnati and Hamilton Railroad | B&O | 1846 | 1847 | Cincinnati, Hamilton and Dayton Railroad | - | |
| Cincinnati, Hamilton and Dayton Railroad | B&O | 1847 | 1895 | Cincinnati, Hamilton and Dayton Railway | - | |
| Cincinnati, Hamilton and Dayton Railway | B&O | 1895 | 1917 | Toledo and Cincinnati Railroad | - | |
| Cincinnati, Hamilton and Indianapolis Railroad | B&O | 1872 | 1902 | Cincinnati, Indianapolis and Western Railway | - | |
| Cincinnati and Indiana Railroad | NYC | 1861 | 1880 | Cincinnati, Indianapolis, St. Louis and Chicago Railway | - | |
| Cincinnati and Indiana Western Railroad | C&O | 1902 | 1903 | Chicago, Cincinnati and Louisville Railroad | - | |
| Cincinnati, Indianapolis, St. Louis and Chicago Railway | NYC | 1880 | 1889 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Cincinnati, Indianapolis and Western Railroad | CIWN | B&O | 1915 | 1990 | CSX Transportation | - |
| Cincinnati, Indianapolis and Western Railway | B&O | 1902 | 1915 | Cincinnati, Indianapolis and Western Railroad | - | |
| Cincinnati Inter-Terminal Railroad | C&O | 1902 | Still exists as a nonoperating subsidiary of CSX Transportation | - | ||
| Cincinnati, Jackson and Mackinaw Railroad | NYC | 1886 | 1892 | Cincinnati, Jackson and Mackinaw Railway | - | |
| Cincinnati, Jackson and Mackinaw Railway | NYC | 1892 | 1897 | Cincinnati Northern Railroad | - | |
| Cincinnati, Lebanon and Northern Railway | PRR | 1885 | 1926 | Pennsylvania, Ohio and Detroit Railroad | - | |
| Cincinnati, Lebanon and Xenia Railroad | PRR | 1850 | 1869 | Miami Valley Narrow Gauge Railway | - | |
| Cincinnati and Michigan Railroad | NYC | 1891 | 1892 | Cincinnati, Jackson and Mackinaw Railway | - | |
| Cincinnati and Muskingum Valley Railroad | PRR | 1898 | 1911 | Cleveland, Akron and Cincinnati Railway | - | |
| Cincinnati and Muskingum Valley Railway | PRR | 1870 | 1898 | Cincinnati and Muskingum Valley Railroad | - | |
| Cincinnati Northern Railroad | CNOR | NYC | 1894 | 1938 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - |
| Cincinnati Northern Railway | PRR | 1880 | 1883 | Toledo, Cincinnati and St. Louis Railroad | - | |
| Cincinnati Northwestern Railway | - | |||||
| Cincinnati and Portsmouth Railroad | - | |||||
| Cincinnati, Portsmouth and Parkersburg Railway | B&O | 1899 | 1899 | Baltimore and Ohio Southwestern Railroad | - | |
| Cincinnati, Portsmouth and Virginia Railroad | N&W | 1891 | 1901 | Norfolk and Western Railway | - | |
| Cincinnati Railway Tunnel Company | PRR | 1872 | 1896 | Cincinnati, Lebanon and Northern Railway | - | |
| Cincinnati and Richmond Railroad | PRR | 1881 | 1890 | Pittsburgh, Cincinnati, Chicago and St. Louis Railway | - | |
| Cincinnati, Richmond and Chicago Railroad | PRR | 1866 | 1890 | Cincinnati and Richmond Railroad | - | |
| Cincinnati, Sandusky and Cleveland Railroad | NYC | 1868 | 1938 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Cincinnati Southern Railway | SOU | 1869 | Still exists as a lessor of Norfolk Southern Railway operating subsidiary Cincinnati, New Orleans and Texas Pacific Railway | |||
| Cincinnati and Springfield Railway | NYC | 1870 | 1912 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Cincinnati Terminal Railway | CTER | 1994 | 1997 | Indiana and Ohio Railway | - | |
| Cincinnati, Van Wert and Michigan Railroad | NYC | 1881 | 1886 | Cincinnati, Jackson and Mackinaw Railroad | - | |
| Cincinnati, Washington and Baltimore Railroad | B&O | 1883 | 1889 | Baltimore and Ohio Southwestern Railroad | - | |
| Cincinnati and Westwood Railroad | - | |||||
| Cincinnati, Wheeling and New York Railroad | B&O | 1883 | 1891 | Eastern Ohio Railroad | - | |
| Cincinnati, Wilmington and Zanesville Railroad | PRR | 1851 | 1863 | Cincinnati and Zanesville Railroad | - | |
| Cincinnati and Zanesville Railroad | PRR | 1864 | 1869 | Cincinnati and Muskingum Valley Railway | - | |
| Cleveland, Akron and Cincinnati Railway | PRR | 1911 | 1926 | Pennsylvania, Ohio and Detroit Railroad | - | |
| Cleveland, Akron and Columbus Railroad | PRR | 1881 | 1882 | Cleveland, Akron and Columbus Railway | - | |
| Cleveland, Akron and Columbus Railway | PRR | 1885 | 1911 | Cleveland, Akron and Cincinnati Railway | - | |
| Cleveland, Barberton and Western Railroad | ACY/ B&O/ ERIE/ PRR | 1899 | 1902 | Akron and Barberton Belt Railroad | - | |
| Cleveland Belt Line Railroad | W&LE | 1890 | 1891 | Cleveland Belt and Terminal Railroad | - | |
| Cleveland Belt and Terminal Railroad | W&LE | 1891 | 1899 | Wheeling and Lake Erie Railroad | - | |
| Cleveland and Canton Railroad | W&LE | 1885 | 1892 | Cleveland, Canton and Southern Railroad | - | |
| Cleveland, Canton, Coshocton and Straitsville Railway | W&LE | 1877 | 1881 | Connotton Valley and Straitsville Railroad | - | |
| Cleveland, Canton and Southern Railroad | W&LE | 1890 | 1899 | Chagrin Falls and Lake Erie Railroad, Wheeling and Lake Erie Railroad | - | |
| Cleveland, Chagrin Falls and Northern Railroad | W&LE | 1888 | 1891 | Cleveland and Canton Railroad | - | |
| Cleveland, Cincinnati, Chicago and St. Louis Railway | NYC | 1889 | 1976 | Consolidated Rail Corporation | - | |
| Cleveland, Columbus and Cincinnati Railroad | NYC | 1836 | 1868 | Cleveland, Columbus, Cincinnati and Indianapolis Railway | - | |
| Cleveland, Columbus, Cincinnati and Indianapolis Railway | NYC | 1868 | 1889 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Cleveland, Delphos and St. Louis Railroad | ACY | 1881 | 1885 | Cleveland and Western Railroad | - | |
| Cleveland, Fort Wayne and Chicago Railroad | NKP | 1865 | 1879 | Ohio Railway | - | |
| Cleveland, Lorain and Wheeling Railroad | B&O | 1883 | 1893 | Cleveland, Lorain and Wheeling Railway | - | |
| Cleveland, Lorain and Wheeling Railway | B&O | 1893 | 1915 | Baltimore and Ohio Railroad | - | |
| Cleveland and Mahoning Railroad | ERIE | 1848 | 1872 | Cleveland and Mahoning Valley Railroad | - | |
| Cleveland and Mahoning Railway | PRR | 1886 | 1911 | Toledo, Columbus and Ohio River Railroad | - | |
| Cleveland and Mahoning Valley Railroad | ERIE | 1872 | 1941 | Erie Railroad | - | |
| Cleveland and Marietta Railroad | PRR | 1879 | 1886 | Cleveland and Marietta Railway | - | |
| Cleveland and Marietta Railway | PRR | 1886 | 1911 | Toledo, Columbus and Ohio River Railroad | - | |
| Cleveland, Medina and Tuscarawas Railroad | B&O | 1853 | 1870 | Lake Shore and Tuscarawas Valley Railway | - | |
| Cleveland, Mount Vernon and Delaware Railroad | PRR | 1869 | 1881 | Cleveland, Akron and Columbus Railroad | - | |
| Cleveland and New Castle Railway | PRR | 1898 | 1899 | Youngstown and Ravenna Railroad | - | |
| Cleveland, Painesville and Ashtabula Railroad | NYC | 1848 | 1868 | Lake Shore Railway | - | |
| Cleveland and Pittsburgh Railroad | PRR | 1836 | 1976 | Consolidated Rail Corporation | - | |
| Cleveland Short Line Railway | NYC | 1902 | 1915 | New York Central Railroad | - | |
| Cleveland and Southwestern Railway | B&O | 1887 | 1893 | Cleveland, Lorain and Wheeling Railway | - | |
| Cleveland and State Line Railroad | NKP | 1887 | 1887 | New York, Chicago and St. Louis Railroad | - | |
| Cleveland Terminal and Valley Railway | B&O | 1895 | 1915 | Baltimore and Ohio Railroad | - | |
| Cleveland and Toledo Railroad | NYC | 1853 | 1869 | Lake Shore Railway | - | |
| Cleveland, Toledo and Lakeside Railway | 1885 | 1886 | Lakeside and Marblehead Railroad | - | ||
| Cleveland, Tuscarawas Valley and Wheeling Railway | B&O | 1875 | 1883 | Cleveland, Lorain and Wheeling Railroad | - | |
| Cleveland Union Terminals Company | - | |||||
| Cleveland and Western Railroad | ACY | 1884 | 1890 | Pittsburgh, Akron and Western Railroad | - | |
| Cleveland and Wheeling Railroad | NYC | 1887 | 1895 | Wheeling and Cleveland Railroad | - | |
| Cleveland, Wooster and Muskingum Valley Railroad | B&O | 1890 | 1915 | Baltimore and Ohio Railroad | - | |
| Cleveland, Youngstown and Pittsburgh Railroad | NYC | 1881 | 1882 | Cleveland, Youngstown and Pittsburgh Railway | - | |
| Cleveland, Youngstown and Pittsburgh Railway | NYC | 1882 | 1886 | Lake Erie, Alliance and Southern Railway | - | |
| Cleveland, Zanesville and Cincinnati Railroad | PRR | 1853 | 1864 | Pittsburgh, Fort Wayne and Chicago Railway | - | |
| College Hill Railroad | - | |||||
| Columbus, Chicago and Indiana Central Railway | PRR | 1868 | 1883 | Chicago, St. Louis and Pittsburgh Railroad | - | |
| Columbus and Cincinnati Railroad | B&O | 1882 | 1882 | Columbus and Cincinnati Midland Railroad | - | |
| Columbus and Cincinnati Midland Railroad | B&O | 1882 | 1915 | Baltimore and Ohio Railroad | - | |
| Columbus Connecting and Terminal Railroad | N&W | 1892 | 1913 | Norfolk and Western Railway | - | |
| Columbus and Eastern Railroad | NYC | 1882 | 1890 | Columbus, Shawnee and Hocking Railway | - | |
| Columbus, Findlay and Northern Railroad | B&O | 1887 | 1943 | Toledo and Cincinnati Railroad | - | |
| Columbus and Gallipolis Railway | C&O | 1876 | 1878 | Ohio and West Virginia Railway | - | |
| Columbus and Hocking Valley Railroad | C&O | 1867 | 1881 | Columbus, Hocking Valley and Toledo Railway | - | |
| Columbus, Hocking Valley and Toledo Railway | C&O | 1881 | 1899 | Hocking Valley Railway | - | |
| Columbus and Indiana Central Railway | PRR | 1867 | 1868 | Columbus, Chicago and Indiana Central Railway | - | |
| Columbus and Indianapolis Railroad | PRR | 1863 | 1864 | Columbus and Indianapolis Central Railway | - | |
| Columbus and Indianapolis Central Railway | PRR | 1864 | 1867 | Columbus and Indiana Central Railway | - | |
| Columbus and Lake Erie Railroad | B&O | 1845 | 1854 | Sandusky, Mansfield and Newark Railroad | - | |
| Columbus, Lancaster and Wellston Railway | - | |||||
| Columbus and Maysville Railroad | N&W | 1881 | 1901 | Cincinnati, Portsmouth and Virginia Railroad | - | |
| Columbus and Maysville Railroad, Southern Division | N&W | 1877 | 1881 | Columbus and Maysville Railroad | - | |
| Columbus Northwestern Railway | DT&I, NYC | 1897 | 1898 | Detroit and Lima Northern Railway | - | |
| Columbus, Piqua and Indiana Railroad | PRR | 1849 | 1863 | Columbus and Indianapolis Railroad | - | |
| Columbus, Sandusky and Hocking Railroad | NYC, PRR | 1895 | 1902 | Toledo, Walhonding Valley and Ohio Railroad, Zanesville and Western Railway | - | |
| Columbus, Sandusky and Hocking Railway | NYC, PRR | 1893 | 1895 | Columbus, Sandusky and Hocking Railroad | - | |
| Columbus, Shawnee and Hocking Railway | NYC | 1889 | 1893 | Columbus, Sandusky and Hocking Railway | - | |
| Columbus and Southern Railway | - | |||||
| Columbus, Springfield and Cincinnati Railroad | NYC | 1869 | 1912 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Columbus and Sunday Creek Valley Railroad | NYC | 1878 | 1879 | Ohio Central Railroad | - | |
| Columbus Terminal and Transfer Railroad | N&W | 1893 | 1905 | Norfolk and Western Railway | - | |
| Columbus, Tiffin and Toledo Railroad | PRR | 1867 | 1870 | Baltimore and Ohio, Toledo and Michigan Railroad | - | |
| Columbus and Toledo Railroad | C&O | 1872 | 1881 | Columbus, Hocking Valley and Toledo Railway | - | |
| Columbus, Washington and Cincinnati Railroad | DT&I | 1877 | 1879 | Cincinnati, Columbus and Hocking Valley Railway | - | |
| Columbus, Wellston and Southern Railroad | - | |||||
| Columbus and Xenia Railroad | PRR | 1844 | 1943 | Little Miami Railroad | - | |
| Conneaut Terminal Railroad | B&LE | 1892 | 1893 | Pittsburgh, Shenango and Lake Erie Railroad | - | |
| Connecting Railway | PRR | 1956 | 1976 | Consolidated Rail Corporation | - | |
| Connotton Northern Railway | W&LE | 1880 | 1880 | Connotton Valley Railway | - | |
| Connotton Valley Railroad | W&LE | 1879 | 1880 | Connotton Valley Railway | - | |
| Connotton Valley Railway | CVRI | 2002 | 2004 | Cleveland Commercial Railroad | - | |
| Connotton Valley Railway | W&LE | 1880 | 1885 | Cleveland and Canton Railroad | - | |
| Connotton Valley and Straitsville Railroad | W&LE | 1881 | 1882 | Connotton Valley Railway | - | |
| Consolidated Rail Corporation | CR | 1976 | 1999 | CSX Transportation, Norfolk Southern Railway | - | |
| Coshocton, Otsego and Eastern Railroad | W&LE | 1909 | 1917 | Wheeling and Lake Erie Railway | - | |
| Coshocton and Southern Railroad | W&LE | 1885 | 1891 | Cleveland, Canton and Southern Railroad | - | |
| Coshocton, Wooster and Cleveland Railroad | B&O | 1851 | 1853 | Cleveland, Medina and Tuscarawas Railroad | - | |
| Covington and Cincinnati Elevated Railroad and Transfer and Bridge Company | C&O | 1886 | 1985 | Chesapeake and Ohio Railway | - | |
| Culver and Port Clinton Railroad | - | |||||
| Cuyahoga Valley Railway | CUVA | 1905 | 2002 | Cleveland Works Railway | - | |
| Dayton and Chicago Railway | B&O | 1886 | 1887 | Dayton, Fort Wayne and Chicago Railway | - | |
| Dayton and Cincinnati Railroad | PRR | 1849 | 1872 | Cincinnati Railway Tunnel Company | - | |
| Dayton and Cincinnati Terminal Railroad | NYC | 1894 | 1894 | Cincinnati Northern Railroad | - | |
| Dayton, Covington and Toledo Railroad | B&O | 1877 | 1880 | Toledo, Delphos and Burlington Railroad | - | |
| Dayton, Fort Wayne and Chicago Railway | B&O | 1887 | 1891 | Cincinnati, Dayton and Chicago Railroad, Cincinnati, Dayton and Ironton Railroad | - | |
| Dayton, Hocking Valley and Eastern Railway | C&O | 1890 | 1900 | Hocking Valley Railway | - | |
| Dayton and Ironton Railroad | B&O | 1884 | 1887 | Dayton, Fort Wayne and Chicago Railway | - | |
| Dayton, Lebanon and Cincinnati Railroad | PRR | 1889 | 1907 | Dayton, Lebanon and Cincinnati Railroad and Terminal Company | - | |
| Dayton, Lebanon and Cincinnati Railroad and Terminal Company | PRR | 1907 | 1915 | Cincinnati, Lebanon and Northern Railway | - | |
| Dayton, Lebanon and Deerfield Railroad | PRR | 1847 | 1848 | Dayton, Springboro, Lebanon and Cincinnati Railroad | - | |
| Dayton and Michigan Railroad | B&O | 1851 | Still exists as a nonoperating subsidiary of CSX Transportation | |||
| Dayton Northern Railway | DT&I | 1895 | 1898 | Detroit and Lima Northern Railway | - | |
| Dayton and Northern Railway | B&O | 1886 | 1886 | Dayton and Chicago Railway | - | |
| Dayton and South Eastern Railroad | B&O | 1871 | 1881 | Toledo, Delphos and Burlington Railroad | - | |
| Dayton, Springboro, Lebanon and Cincinnati Railroad | PRR | 1848 | 1849 | Dayton and Cincinnati Railroad | - | |
| Dayton and Toledo Railroad | B&O | 1885 | 1886 | Dayton and Northern Railway | - | |
| Dayton, Toledo and Chicago Railway | - | |||||
| Dayton Union Railway | B&O/ NYC/ PRR | 1892 | 1976 | Consolidated Rail Corporation | - | |
| Dayton and Union Railroad | B&O | 1863 | 1989 | CSX Transportation | - | |
| Dayton and Western Railroad | PRR | 1846 | 1943 | Little Miami Railroad | - | |
| Dayton, Xenia and Belpre Railroad | PRR | 1851 | 1865 | Columbus and Xenia Railroad, Little Miami Railroad | - | |
| Delphos and Kokomo Railroad | NKP | 1877 | 1879 | Toledo, Delphos and Burlington Railroad | - | |
| Detroit, Butler and St. Louis Railroad | WAB | 1881 | 1881 | Wabash, St. Louis and Pacific Railway | - | |
| Detroit and Chicago Railroad | NYC | 1888 | 1914 | New York Central Railroad | - | |
| Detroit and Lima Northern Railway | DT&I, NYC | 1897 | 1901 | Detroit Southern Railroad, Toledo and Ohio Central Railway | - | |
| Detroit Southern Railroad | DT&I | 1901 | 1905 | Detroit, Toledo and Ironton Railway | - | |
| Detroit and State Line Wabash Railroad | WAB | 1889 | 1889 | Wabash Railroad | - | |
| Detroit, Toledo and Ironton Railroad | DT&I, DTI | DT&I | 1914 | 1983 | Grand Trunk Western Railroad | - |
| Detroit, Toledo and Ironton Railway | DT&I | 1905 | 1913 | Detroit, Toledo and Ironton Railroad | - | |
| Detroit and Toledo Shore Line Railroad | D&TS, DTS | CN/ NKP | 1899 | 1981 | Grand Trunk Western Railroad | - |
| Eastern Ohio Railroad | B&O | 1891 | 1915 | Baltimore and Ohio Railroad | - | |
| Eastern Ohio Railroad | B&O | 1871 | 1882 | Wheeling and Cincinnati Mineral Railway | - | |
| Eaton and Hamilton Railroad | PRR | 1847 | 1866 | Cincinnati, Richmond and Chicago Railroad | - | |
| Elyria and Black River Railway | B&O | 1871 | 1872 | Lake Shore and Tuscarawas Valley Railway | - | |
| Erie Railroad | ERIE | ERIE | 1895 | 1960 | Erie–Lackawanna Railroad | - |
| Erie and Kalamazoo Railroad | NYC | 1833 | 1976 | Consolidated Rail Corporation | - | |
| Erie–Lackawanna Railroad | EL | 1960 | 1968 | Erie Lackawanna Railway | - | |
| Erie Lackawanna Railway | EL | 1968 | 1976 | Consolidated Rail Corporation | - | |
| Euclid Railroad | 1883 | 1967 | N/A | - | ||
| Fairport, Painesville and Eastern Railroad | FP&E, FPE | N&W | 1910 | 1984 | Norfolk and Western Railway | - |
| Federal Valley Railroad | NYC | 1918 | 1952 | New York Central Railroad | - | |
| Felicity and Bethel Railroad | - | |||||
| Findlay Belt Railway | NYC | 1887 | 1932 | N/A | - | |
| Findlay, Fort Wayne and Western Railroad | 1890 | 1894 | Ohio Railway | - | ||
| Findlay, Fort Wayne and Western Railway | 1894 | 1903 | Cincinnati, Findlay and Fort Wayne Railway | - | ||
| Findlay and Western Railroad | 1890 | 1890 | Findlay, Fort Wayne and Western Railroad | - | ||
| Flint and Pere Marquette Railroad | PM | 1897 | 1899 | Pere Marquette Railroad | - | |
| Franklin and Ohio River Railroad | - | |||||
| Franklin and Warren Railroad | ERIE | 1851 | 1853 | Atlantic and Great Western Railroad | - | |
| Fremont and Indiana Railroad | NKP | 1853 | 1861 | Fremont, Lima and Union Railroad | - | |
| Fremont, Lima and Union Railroad | NKP | 1862 | 1865 | Lake Erie and Louisville Railroad | - | |
| Fremont, Lima and Union Railway | NKP | 1871 | 1872 | Lake Erie and Louisville Railway | - | |
| Gallipolis, McArthur and Columbus Railroad | C&O | 1870 | 1877 | Columbus and Gallipolis Railway | - | |
| Grafton and Brunswick Railroad | - | |||||
| Great Miami and Scioto Railway | GMRY | 1994 | 2004 | U S Rail Corporation | - | |
| Greenville and Miami Railroad | B&O | 1846 | 1862 | Dayton and Union Railroad | - | |
| Hamilton Belt Railway | B&O | 1896 | 1944 | Baltimore and Ohio Railroad | - | |
| Harrison Branch Company | NYC | 1864 | 1871 | Harrison Branch Railroad | - | |
| Harrison Branch Railroad | NYC | 1871 | 1912 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Harrison, Jefferson and Belmont Railway | W&LE | 1887 | 1888 | Jefferson and Columbiana Railway | - | |
| Hillsboro Railroad | N&W | 1895 | 1902 | Norfolk and Western Railway | - | |
| Hillsborough and Cincinnati Railroad | B&O | 1846 | 1860 | Marietta and Cincinnati Railroad | - | |
| Hillsboro Short Line Railway | N&W | 1879 | 1902 | Norfolk and Western Railway | - | |
| Hillsdale County Railroad | 1986 | 1992 | Indiana Northeastern Railroad | - | ||
| Hocking Valley Railway | HV | C&O | 1899 | 1930 | Chesapeake and Ohio Railway | - |
| Holliday's Cove Railroad | PRR | 1860 | 1868 | Pittsburg, Cincinnati and St. Louis Railway | - | |
| Home Avenue Railroad | B&O | 1871 | Still exists as a nonoperating subsidiary of CSX Transportation | |||
| Huron and Oxford Railroad | B&O | 1846 | 1854 | Sandusky, Mansfield and Newark Railroad | - | |
| Indiana, Bloomington and Western Railway | NYC | 1881 | 1887 | Springfield and Western Railway | - | |
| Indiana Hi-Rail Corporation | IHRC | 1980 | 1997 | Delphos Terminal Company, Maumee and Western Railroad | - | |
| Indiana and Ohio Railroad | INOH | 1979 | 1997 | Indiana and Ohio Railway | - | |
| Indiana and Ohio Central Railroad | IOCR | 1987 | 2005 | Indiana and Ohio Railway | - | |
| Indiana and Ohio Eastern Railroad | 1987 | 1994 | Great Miami and Scioto Railway | - | ||
| Indianapolis and Cincinnati Railroad | NYC | 1866 | 1867 | Indianapolis, Cincinnati and La Fayette Railway | - | |
| Indianapolis, Cincinnati and La Fayette Railroad | NYC | 1873 | 1880 | Cincinnati, Indianapolis, St. Louis and Chicago Railway | - | |
| Indianapolis, Cincinnati and La Fayette Railway | NYC | 1867 | 1873 | Indianapolis, Cincinnati and La Fayette Railroad | - | |
| Industrial Railroad | ERIE/ PRR | 1903 | 1910 | Lorain, Ashland and Southern Railroad | - | |
| Iron Railroad | DT&I | 1849 | 1881 | Toledo, Delphos and Burlington Railroad | - | |
| Iron Railway | DT&I | 1884 | 1902 | Detroit Southern Railroad | - | |
| Island Creek and Richmond Mineral Railroad | NYC | 1877 | 1881 | Steubenville, Canton and Cleveland Railway | - | |
| Ivorydale and Mill Creek Valley Railway | - | |||||
| Jefferson and Columbiana Railway | W&LE | 1888 | 1888 | Wheeling and Lake Erie Railway | - | |
| Junction Railroad | B&O | 1849 | 1878 | Cincinnati, Hamilton and Indianapolis Railroad | - | |
| Junction Railroad | NYC | 1846 | 1853 | Cleveland and Toledo Railroad | - | |
| Junction Railway | NYC | 1872 | 1872 | Toledo, Canada Southern and Detroit Railway | - | |
| Kanawha and Michigan Railway | K&M | NYC | 1890 | 1938 | Toledo and Ohio Central Railway | - |
| Kanawha and Ohio Railway | NYC | 1886 | 1890 | Kanawha and Michigan Railway | - | |
| Lake Erie, Alliance and Southern Railway | NYC | 1887 | 1893 | Alliance and Northern Railroad, Ohio River and Lake Erie Railroad | - | |
| Lake Erie, Alliance and Wheeling Railroad | NYC | 1901 | 1952 | New York Central Railroad | - | |
| Lake Erie, Alliance and Wheeling Railroad | NYC | 1874 | 1878 | Alliance and Lake Erie Railroad | - | |
| Lake Erie and Eastern Railroad | LEE | PLE | 1904 | 1984 | Pittsburgh and Lake Erie Railroad | - |
| Lake Erie and Louisville Railroad | NKP | 1877 | 1879 | Lake Erie and Western Railway | - | |
| Lake Erie and Louisville Railroad | NKP | 1865 | 1871 | Fremont, Lima and Union Railway | - | |
| Lake Erie and Louisville Railway | NKP | 1872 | 1877 | Lake Erie and Louisville Railroad | - | |
| Lake Erie and Pittsburgh Railway | NYC/ PRR | 1903 | 1970 | Penn Central Transportation Company | - | |
| Lake Erie and Western Railroad | NKP | 1887 | 1923 | New York, Chicago and St. Louis Railroad | - | |
| Lake Erie and Western Railway | NKP | 1879 | 1886 | Lake Erie and Western Railroad | - | |
| Lake Erie, Wooster and Muskingum Valley Railroad | B&O | 1881 | 1891 | Cleveland, Wooster and Muskingum Valley Railroad | - | |
| Lake Erie, Youngstown and Southern Railroad | PLE | 1902 | 1912 | Lake Erie and Eastern Railroad | - | |
| Lake and River Railway | ACY, ERIE/ PRR | 1903 | 1907 | Ashland and Western Railroad, Akron, Canton and Youngstown Railway | - | |
| Lake Shore Railway | NYC | 1868 | 1869 | Lake Shore and Michigan Southern Railway | - | |
| Lake Shore and Michigan Southern Railway | NYC | 1869 | 1914 | New York Central Railroad | - | |
| Lake Shore and Tuscarawas Valley Railway | B&O | 1870 | 1875 | Cleveland, Tuscarawas Valley and Wheeling Railway | - | |
| Lake View and Collamer Railroad | 1875 | 1882 | New York, Chicago and St. Louis Railroad | Operated as Cleveland, Painesville and Ashtabula Railroad from 1879 to 1882 | ||
| Lakeside and Marblehead Railroad | 1886 | 1964 | N/A | - | ||
| Lancaster and Hamden Railway | - | |||||
| Lawrence Railroad | PRR | 1865 | 1887 | Youngstown, Lawrence and Pittsburgh Railroad | - | |
| Lawrence Railroad and Transportation Company | PRR | 1864 | 1865 | Lawrence Railroad | - | |
| Liberty and Vienna Railroad | ERIE | 1868 | 1872 | Cleveland and Mahoning Valley Railway | - | |
| Lima Belt Railway | B&O | 1900 | 1944 | Baltimore and Ohio Railroad | - | |
| Lima Northern Railway | DT&I | 1895 | 1897 | Detroit and Lima Northern Railway | - | |
| Little Miami Railroad | PRR | 1836 | 1976 | Consolidated Rail Corporation | - | |
| Lorain and Ashland Railroad | ERIE/ PRR | 1905 | 1910 | Lorain, Ashland and Southern Railroad | - | |
| Lorain, Ashland and Southern Railroad | LA&S | ERIE/ PRR | 1910 | 1925 | N/A | - |
| Lorain and Southern Railroad | 1903 | 1952 | N/A | - | ||
| Lorain and West Virginia Railway | LAWV | W&LE | 1906 | 1983 | N/A | - |
| Louisville, Cincinnati and Dayton Railway | B&O | 1886 | 1888 | Cincinnati and Dayton Railway | - | |
| Louisville, Cincinnati and Lexington Railroad | L&N | 1872 | 1877 | Louisville, Cincinnati and Lexington Railway | - | |
| Louisville, Cincinnati and Lexington Railway | L&N | 1877 | 1881 | Louisville and Nashville Railroad | - | |
| Louisville and Nashville Railroad | L&N, LN | L&N | 1881 | 1983 | Seaboard System Railroad | - |
| Mad River and Lake Erie Railroad | NYC | 1832 | 1858 | Sandusky, Dayton and Cincinnati Railroad | - | |
| Mahoning Coal Railroad | NYC | 1871 | 1976 | Consolidated Rail Corporation | - | |
| Mahoning and Shenango Valley Railway | NYC | 1886 | 1976 | Consolidated Rail Corporation | - | |
| Mahoning State Line Railroad | PLE | 1891 | Still exists as a nonoperating subsidiary of CSX Transportation | |||
| Mahoning Valley Western Railway | B&O | 1902 | 1915 | Baltimore and Ohio Railroad | - | |
| Mansfield, Coldwater and Lake Michigan Railroad | PRR | 1871 | 1877 | Northwestern Ohio Railway | - | |
| Mansfield, Coldwater and Lake Michigan Railway | PRR | 1870 | 1871 | Mansfield, Coldwater and Lake Michigan Railroad | - | |
| Mansfield and New Haven Railroad | B&O | 1836 | 1843 | Mansfield and Sandusky City Railroad | - | |
| Mansfield and Sandusky City Railroad | B&O | 1843 | 1854 | Sandusky, Mansfield and Newark Railroad | - | |
| Manufacturers Railway | PRR | 1894 | 1926 | Pennsylvania, Ohio and Detroit Railroad | - | |
| Marietta Railway | B&O | 1896 | 1900 | Baltimore and Ohio Southwestern Railroad | - | |
| Marietta and Cincinnati Railroad | B&O | 1851 | 1882 | Cincinnati, Washington and Baltimore Railroad | - | |
| Marietta, Columbus and Cleveland Railroad | 1900 | 1916 | Federal Valley Railroad, Marietta and Vincent Railroad | - | ||
| Marietta, Columbus and Northern Railroad | - | |||||
| Marietta, Hocking and Northern Railway | - | |||||
| Marietta and Pittsburgh Railroad | PRR | 1868 | 1873 | Marietta, Pittsburg and Cleveland Railway | - | |
| Marietta, Pittsburg and Cleveland Railway | PRR | 1873 | 1877 | Cleveland and Marietta Railroad | - | |
| Marietta and Vincent Railroad | 1900 | - | ||||
| Martins Ferry Terminal Railroad | PRR | 1888 | 1892 | Wheeling Bridge and Terminal Railway | - | |
| Massillon Railroad | W&LE | 1892 | 1904 | Wheeling and Lake Erie Railroad | - | |
| Massillon Belt Railway | 1909 | 1929 | N/A | - | ||
| Massillon and Cleveland Railroad | PRR | 1868 | 1928 | Pittsburgh, Fort Wayne and Chicago Railway | - | |
| Massillon and Coshocton Railway | W&LE | 1874 | 1877 | Cleveland, Canton, Coshocton and Straitsville Railway | - | |
| McComb, Deshler and Toledo Railroad | B&O | 1879 | 1887 | Columbus, Findlay and Northern Railroad | - | |
| Miami Valley Railway | PRR | 1876 | 1880 | Cincinnati Northern Railway | - | |
| Miami Valley Narrow Gauge Railway | PRR | 1874 | 1876 | Miami Valley Railway | - | |
| Michigan Central Railroad | MC | NYC | 1883 | 1976 | Consolidated Rail Corporation | - |
| Michigan Interstate Railway | 1977 | 1988 | Ann Arbor Railroad | - | ||
| Michigan and Ohio Railroad | NYC | 1883 | 1887 | Cincinnati, Jackson and Mackinaw Railroad | - | |
| Michigan Southern and Northern Indiana Railroad | NYC | 1855 | 1869 | Lake Shore and Michigan Southern Railway | - | |
| Middleport and Northeastern Railway | NYC | 1913 | 1938 | Toledo and Ohio Central Railway | - | |
| Middletown and Cincinnati Railroad | PRR | 1894 | 1902 | Cincinnati, Lebanon and Northern Railway | - | |
| Middletown and Cincinnati Railway | PRR | 1890 | 1894 | Middletown and Cincinnati Railroad | - | |
| Middletown and State Line Railroad | B&O | 1886 | 1886 | Louisville, Cincinnati and Dayton Railway | - | |
| Midway Railroad | MID | 1963 | 1973 | N/A | - | |
| Millersburgh, Jeromeville and Greenwich Railroad | ERIE/ PRR | 1894 | 1897 | Ashland and Wooster Railway | - | |
| Mineral Railroad | C&O | 1864 | 1867 | Columbus and Hocking Valley Railroad | - | |
| Monroe and Toledo Railway | PM | 1893 | 1897 | Flint and Pere Marquette Railroad | - | |
| Monroeville and Sandusky City Railroad | B&O | 1835 | 1843 | Sandusky, Mansfield and Newark Railroad | - | |
| Montpelier and Chicago Railroad | WAB | 1889 | 1891 | Wabash Railroad | - | |
| Morgan Run Railway | - | |||||
| Mount Gilead Short Line Railway | NYC | 1879 | 1966 | N/A | - | |
| Moxahala Valley Railway | MOXV | 1983 | 1986 | Ohio Southern Railroad | - | |
| Muskingum County Railway | PRR | 1881 | 1893 | Bellaire, Zanesville and Cincinnati Railway | - | |
| New Castle and Ohio River Railway | - | |||||
| New Lisbon Railway | ERIE | 1864 | 1869 | Niles and New Lisbon Railway | - | |
| New York Central Railroad | NYC | NYC | 1914 | 1968 | Penn Central Transportation Company | - |
| New York and Chicago Railway of Ohio | NKP | 1881 | 1881 | New York, Chicago and St. Louis Railway | - | |
| New York, Chicago and St. Louis Railroad | NKP | NKP | 1887 | 1964 | Norfolk and Western Railway | - |
| New York, Chicago and St. Louis Railway | NKP | 1881 | 1887 | Cleveland and State Line Railroad | - | |
| New York, Fort Wayne and Chicago Railroad | NKP | 1880 | 1881 | New York, Chicago and St. Louis Railway | - | |
| New York, Lake Erie and Western Railroad | ERIE | 1883 | 1895 | Erie Railroad | - | |
| New York, Mahoning and Western Railroad | 1887 | 1889 | American Midland Railway | - | ||
| New York, Pennsylvania and Ohio Railroad | ERIE | 1880 | 1896 | Nypano Railroad | - | |
| New York, Pittsburgh and Chicago Railway | PLE/ PRR | 1881 | 1885 | Pittsburgh, Marion and Chicago Railway | - | |
| Newark, Somerset and Straitsville Railroad | B&O | 1867 | 1900 | Ohio Midland Railroad | - | |
| Newburgh and South Shore Railway | N&SS, NSS | 1899 | 1986 | Cuyahoga Valley Railway, Newburgh and South Shore Railroad | - | |
| Newport and Cincinnati Bridge Company | L&N | 1868 | 1904 | Louisville and Nashville Railroad | - | |
| Niles and New Lisbon Railway | ERIE | 1869 | 1872 | Cleveland and Mahoning Valley Railway | - | |
| Nimishillen and Tuscarawas Railway | NTRY | 1990 | 2003 | Republic N&T Railroad | - | |
| Norfolk and Western Railroad | N&W | 1890 | 1896 | Norfolk and Western Railway | - | |
| Norfolk and Western Railway | N&W, NW | N&W | 1896 | 1998 | Norfolk Southern Railway | - |
| North-Eastern Ohio Railroad | B&LE | 1888 | 1888 | Pittsburgh, Shenango and Lake Erie Railroad | - | |
| North Western Ohio Railroad | WAB | 1880 | 1880 | Butler and Detroit Railroad | - | |
| North Western Ohio Railroad | NYC | 1871 | 1871 | Chicago and Canada Southern Railway | - | |
| Northern Indiana Railroad | NYC | 1851 | 1855 | Michigan Southern and Northern Indiana Railroad | - | |
| Northern Ohio Railway | ACY | 1895 | 1944 | Akron, Canton and Youngstown Railroad | - | |
| Northwestern Ohio Railway | PRR | 1876 | 1891 | Toledo, Walhonding Valley and Ohio Railroad | - | |
| Nypano Railroad | ERIE | 1896 | 1941 | Erie Railroad | - | |
| Oberlin and La Grange Railway | - | |||||
| Ohio Railroad | NYC | 1836 | 1852 | Junction Railroad | - | |
| Ohio Railway | 1894 | 1894 | Findlay, Fort Wayne and Western Railway | - | ||
| Ohio Railway | ACY | 1883 | 1887 | Pittsburgh, Akron and Western Railway | - | |
| Ohio Railway | NKP | 1879 | 1880 | New York, Fort Wayne and Chicago Railroad | - | |
| Ohio Central Railroad | NYC | 1879 | 1885 | Ohio and Kanawha Railway, Toledo and Ohio Central Railway | - | |
| Ohio Central Railway | NYC | 1876 | 1878 | Columbus and Sunday Creek Valley Railroad, Ohio Central Railroad | - | |
| Ohio and Indiana Railroad | PRR | 1850 | 1856 | Pittsburgh, Fort Wayne and Chicago Railroad | - | |
| Ohio, Indiana and Missouri Railroad | 1889 | American Midland Railway | - | |||
| Ohio, Indiana and Pacific Railway | NYC | 1881 | 1881 | Indiana, Bloomington and Western Railway | - | |
| Ohio and Indiana State Line Railroad | NYC | 1880 | 1881 | Ohio, Indiana and Pacific Railway | - | |
| Ohio, Indiana and Western Railway | NYC | 1887 | 1890 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - | |
| Ohio and Kanawha Railway | NYC | 1886 | 1886 | Kanawha and Ohio Railway | - | |
| Ohio and Kentucky Bridge Company | C&O | 1886 | 1886 | Covington and Cincinnati Elevated Railroad and Transfer and Bridge Company | - | |
| Ohio and Little Kanawha Railroad | B&O | 1900 | 1966 | Baltimore and Ohio Railroad | - | |
| Ohio Midland Railroad | B&O | 1900 | 1915 | Baltimore and Ohio Railroad | - | |
| Ohio and Mississippi Railroad | B&O | 1848 | 1867 | Ohio and Mississippi Railway | - | |
| Ohio and Mississippi Railway | B&O | 1867 | 1893 | Baltimore and Ohio Southwestern Railway | - | |
| Ohio and Morenci Railroad | 1933 | 1954 | N/A | - | ||
| Ohio and North Western Railroad | N&W | 1886 | 1890 | Cincinnati, Portsmouth and Virginia Railroad | - | |
| Ohio and Pennsylvania Railroad | PRR | 1848 | 1856 | Pittsburgh, Fort Wayne and Chicago Railroad | - | |
| Ohio and Pennsylvania Belt Line Railroad | - | |||||
| Ohio and Toledo Railroad | W&LE | 1872 | 1878 | Youngstown and Connotton Valley Railroad | - | |
| Ohio River Railway | N&W | 1877 | 1890 | Scioto Valley Railway | - | |
| Ohio River and Columbus Railway | - | |||||
| Ohio River and Lake Erie Railroad | NYC | 1897 | 1901 | Lake Erie, Alliance and Wheeling Railroad | - | |
| Ohio River and Western Railway | PRR | 1902 | 1931 | Pittsburgh, Ohio Valley and Cincinnati Railroad | - | |
| Ohio Southern Railroad | DT&I | 1881 | 1898 | Detroit Southern Railroad | - | |
| Ohio Valley Railway | PRR | 1871 | 1890 | Pittsburgh, Ohio Valley and Cincinnati Railroad | - | |
| Ohio Valley and Junction Railway | PRR | 1897 | 1902 | Cleveland and Mahoning Railway | - | |
| Ohio and West Virginia Railway | C&O | 1878 | 1881 | Columbus, Hocking Valley and Toledo Railway | - | |
| Painesville, Canton and Bridgeport Narrow Gauge Railroad | W&LE | 1875 | 1880 | Chagrin Falls and Southern Railroad | - | |
| Painesville and Hudson Railroad | B&O | 1852 | 1870 | Painesville and Youngstown Railroad | - | |
| Painesville, Wooster and Ohio Railway | B&O | 1886 | 1890 | Lake Erie, Wooster and Muskingum Valley Railroad | - | |
| Painesville and Youngstown Railroad | B&O | 1870 | 1879 | Painesville and Youngstown Railway | - | |
| Painesville and Youngstown Railway | B&O | 1879 | 1886 | Pittsburg, Painesville and Fairport Railway | - | |
| Paulding and Cecil Railway | NYC | 1879 | 1887 | Cincinnati, Jackson and Mackinaw Railroad | - | |
| Penn Central Transportation Company | PC | 1968 | 1976 | Consolidated Rail Corporation | - | |
| Penndel Company | PRR | 1954 | 1976 | Consolidated Rail Corporation | - | |
| Pennsylvania Company | PRR | 1871 | 1918 | Pennsylvania Railroad | - | |
| Pennsylvania Railroad | PRR | PRR | 1918 | 1968 | Penn Central Transportation Company | - |
| Pennsylvania, Ohio and Detroit Railroad | PRR | 1926 | 1956 | Connecting Railway | - | |
| Peoria and Eastern Railway | PAE | NYC | 1890 | 1914 | Cleveland, Cincinnati, Chicago and St. Louis Railway | - |
| Pere Marquette Railroad | PM | 1899 | 1917 | Pere Marquette Railway | - | |
| Pere Marquette Railway | PM | PM | 1917 | 1947 | Chesapeake and Ohio Railway | - |
| Philadelphia, Baltimore and Washington Railroad | PRR | 1956 | 1976 | Consolidated Rail Corporation | - | |
| Pioneer and Fayette Railroad | PF | 1933 | 1992 | N/A | - | |
| Piqua and Troy Branch Railroad | B&O | 1881 | 1943 | Toledo and Cincinnati Railroad | - | |
| Pittsburgh, Akron and Western Railroad | ACY | 1890 | 1894 | Northern Ohio Railway | - | |
| Pittsburgh, Akron and Western Railway | ACY | 1887 | 1890 | Pittsburgh, Akron and Western Railroad | - | |
| Pittsburg, Bessemer and Lake Erie Railroad | B&LE | 1897 | 1949 | Bessemer and Lake Erie Railroad | - | |
| Pittsburgh and Chicago Railroad | ACY | 1882 | 1887 | Cleveland and Western Railroad | - | |
| Pittsburgh, Cincinnati, Chicago and St. Louis Railroad | PRR | 1917 | 1956 | Philadelphia, Baltimore and Washington Railroad | - | |
| Pittsburgh, Cincinnati, Chicago and St. Louis Railway | PRR | 1890 | 1917 | Pittsburgh, Cincinnati, Chicago and St. Louis Railroad | - | |
| Pittsburg, Cincinnati and St. Louis Railway | PRR | 1868 | 1890 | Pittsburgh, Cincinnati, Chicago and St. Louis Railway | - | |
| Pittsburg, Cleveland and Toledo Railroad | B&O | 1882 | 1915 | Baltimore and Ohio Railroad | - | |
| Pittsburgh, Fort Wayne and Chicago Railroad | PRR | 1856 | 1861 | Pittsburgh, Fort Wayne and Chicago Railway | - | |
| Pittsburgh, Fort Wayne and Chicago Railway | PRR | 1862 | 1976 | Consolidated Rail Corporation | - | |
| Pittsburgh and Lake Erie Railroad | PLE | PLE | 1878 | 1992 | Three Rivers Railway | - |
| Pittsburgh, Lisbon and Western Railroad | PL&W, PLW | PLE/ PRR | 1902 | 1945 | Youngstown and Southern Railway | - |
| Pittsburgh, Lisbon and Western Railway | PLE/ PRR | 1896 | 1902 | Pittsburgh, Lisbon and Western Railroad | - | |
| Pittsburgh, Marion and Chicago Railway | PLE/ PRR | 1885 | 1896 | Pittsburgh, Lisbon and Western Railway | - | |
| Pittsburg, Maysville and Cincinnati Railroad | B&O | 1853 | 1865 | South Western Railroad | - | |
| Pittsburgh, Mount Vernon, Columbus and London Railroad | PRR | 1869 | 1869 | Cleveland, Mount Vernon and Delaware Railroad | - | |
| Pittsburgh, New Castle and Lake Erie Railroad | B&O | 1877 | 1881 | Pittsburgh and Western Railroad | - | |
| Pittsburgh and Northwestern Railroad | B&O | 1875 | 1877 | Pittsburgh, New Castle and Lake Erie Railroad | - | |
| Pittsburgh, Ohio Valley and Cincinnati Railroad | PRR | 1890 | 1954 | Penndel Company | - | |
| Pittsburg, Painesville and Fairport Railway | B&O | 1886 | 1915 | Baltimore and Ohio Railroad | - | |
| Pittsburgh, Shenango and Lake Erie Railroad | B&LE | 1888 | 1897 | Pittsburg, Bessemer and Lake Erie Railroad | - | |
| Pittsburgh, Toledo and Western Railroad | P&WV | 1901 | 1904 | Wabash Pittsburgh Terminal Railway | - | |
| Pittsburgh and West Virginia Railroad | 1967 | Still exists as a lessor of the Wheeling and Lake Erie Railway | ||||
| Pittsburgh and West Virginia Railway | P&WV | P&WV | 1917 | 1967 | Pittsburgh and West Virginia Railroad | - |
| Pittsburgh and Western Railroad | B&O | 1881 | 1887 | Pittsburgh and Western Railway | - | |
| Pittsburgh and Western Railway | B&O | 1887 | 1902 | N/A | Only entered Ohio through leases of other companies | |
| Pittsburgh, Youngstown and Ashtabula Railroad | PRR | 1887 | 1906 | Pittsburgh, Youngstown and Ashtabula Railway | - | |
| Pittsburgh, Youngstown and Ashtabula Railway | PRR | 1906 | 1976 | Consolidated Rail Corporation | - | |
| Pittsburg, Youngstown and Chicago Railroad | B&O | 1881 | 1882 | Pittsburg, Cleveland and Toledo Railroad | - | |
| Pleasant Bay Railway | CN/ NKP | 1899 | 1899 | Detroit and Toledo Shore Line Railroad | - | |
| PL&W Railroad | PLW | 1993 | 1995 | Ohio and Pennsylvania Railroad | - | |
| Point Pleasant Bridge Company | NYC | 1886 | 1935 | Kanawha and Michigan Railway | - | |
| Point Pleasant and Ohio River Railroad | NYC | 1883 | 1885 | Kanawha and Ohio Railway, Point Pleasant Bridge Company | - | |
| Pomeroy Belt Railway | C&O | 1909 | 1930 | Chesapeake and Ohio Railway | - | |
| Pomeroy and Ohio River Railroad | NYC | 1881 | 1883 | Point Pleasant and Ohio River Railroad | - | |
| Port Clinton Railroad | NYC | 1852 | 1853 | Cleveland and Toledo Railroad | - | |
| Railroad Ventures, Inc. | 1996 | 2001 | Columbiana County Port Authority | - | ||
| Richland and Mahoning Railway | ACY | 1898 | 1903 | Lake and River Railway | - | |
| Richmond and Covington Railroad | PRR | 1862 | 1864 | Columbus and Indianapolis Railroad | - | |
| River Terminal Railway | RT | 1909 | 2002 | Cleveland Works Railway | - | |
| Rocky River Railroad | NKP | 1867 | 1881 | New York, Chicago and St. Louis Railroad | - | |
| Rolling Mill Railroad | PRR | 1883 | 1904 | Toledo, Walhonding Valley and Ohio Railroad | - | |
| St. Clairsville Railway | B&O | 1880 | 1885 | Bellaire and St. Clairsville Railway | - | |
| St. Clairsville and Northern Railway | B&O | 1879 | 1945 | N/A | - | |
| St. Joseph Valley Railway | - | |||||
| Salem Railroad | PLE/ PRR | 1881 | 1902 | Pittsburgh, Lisbon and Western Railroad | - | |
| Sandusky and Cincinnati Railroad | NYC | 1866 | 1868 | Cincinnati, Sandusky and Cleveland Railroad | - | |
| Sandusky City and Indiana Railroad | NYC | 1851 | 1866 | Sandusky and Cincinnati Railroad | - | |
| Sandusky and Columbus, Lake Erie and Southern Short Line Railway | PRR | 1889 | 1891 | Sandusky and Columbus Short Line Railway | - | |
| Sandusky and Columbus Short Line Railway | PRR | 1891 | 1893 | Columbus, Sandusky and Hocking Railway | - | |
| Sandusky, Dayton and Cincinnati Railroad | NYC | 1858 | 1866 | Sandusky and Cincinnati Railroad | - | |
| Sandusky and Fremont Railway | NKP | 1880 | 1880 | Lake Erie and Western Railway | - | |
| Sandusky, Mansfield and Newark Railroad | B&O | 1854 | 1915 | Baltimore and Ohio Railroad | - | |
| Sandyville and Waynesburg Railroad | B&O | 1898 | 1914 | Cleveland Terminal and Valley Railway | - | |
| Scioto and Hocking Valley Railroad | B&O | 1849 | 1863 | Marietta and Cincinnati Railroad, Newark, Somerset and Straitsville Railroad | - | |
| Scioto Valley Railway | N&W | 1875 | 1890 | Scioto Valley and New England Railroad | - | |
| Scioto Valley and New England Railroad | N&W | 1890 | 1890 | Norfolk and Western Railroad | - | |
| Seaboard System Railroad | SBD | 1983 | 1986 | CSX Transportation | - | |
| Sharon Railroad | B&O | 1849 | 1853 | Pittsburg, Maysville and Cincinnati Railroad | - | |
| Silica Northern Railway | 1912 | 1913 | Toledo, Angola and Western Railway | - | ||
| South Park and Royalton Railroad | B&O | 1898 | 1916 | Baltimore and Ohio Railroad | - | |
| South Pennsylvania and Ohio Railway | NYC | 1883 | 1888 | Cleveland and Wheeling Railroad | - | |
| South Western Railroad | B&O | 1865 | 1871 | Eastern Ohio Railroad | - | |
| Spring Grove, Avondale and Cincinnati Railway | 1881 | 1883 | Toledo, Cincinnati and St. Louis Railroad | - | ||
| Springfield and Columbus Railroad | NYC | 1849 | 1868 | Columbus, Springfield and Cincinnati Railroad | - | |
| Springfield, Jackson and Pomeroy Railroad | DT&I | 1874 | 1879 | Springfield Southern Railroad | - | |
| Springfield and Mansfield Railroad | NYC, PRR | 1850 | 1852 | Springfield, Mount Vernon and Pittsburgh Railroad | - | |
| Springfield, Mount Vernon and Pittsburgh Railroad | NYC, PRR | 1852 | 1867 | Cleveland, Columbus and Cincinnati Railroad, Pittsburgh, Mount Vernon, Columbus and London Railroad | - | |
| Springfield Southern Railroad | DT&I | 1879 | 1881 | Ohio Southern Railroad | - | |
| Springfield and Western Railway | NYC | 1887 | 1887 | Ohio, Indiana and Western Railway | - | |
| Sterling Belt Line Railway | SBLN | 1984 | N/A | - | ||
| Steubenville, Canton and Cleveland Railway | NYC | 1881 | 1882 | Cleveland, Youngstown and Pittsburgh Railway | - | |
| Steubenville and Indiana Railroad | PRR | 1848 | 1868 | Pittsburg, Cincinnati and St. Louis Railway | - | |
| Sugar Creek and Northern Railroad | W&LE | Wheeling and Lake Erie Railway | - | |||
| Sunday Creek Railroad | B&O | 1901 | 1930 | N/A | - | |
| Swan Creek Railway of Toledo | NYC | 1875 | 1914 | New York Central Railroad | - | |
| Tiffin and Fort Wayne Railroad | NKP | 1854 | 1859 | American Central Railway | - | |
| Toledo, Angola and Western Railway | TA&W, TAW | 1902 | 1981 | N/A | - | |
| Toledo and Ann Arbor Railroad | AA | 1878 | 1880 | Toledo, Ann Arbor and Grand Trunk Railway | - | |
| Toledo, Ann Arbor and Detroit Railroad | DT&I | 1904 | 1912 | Toledo, Ann Arbor and Jackson Railroad | - | |
| Toledo, Ann Arbor and Grand Trunk Railway | AA | 1880 | 1884 | Toledo, Ann Arbor and North Michigan Railway | - | |
| Toledo, Ann Arbor and Jackson Railroad | DT&I | 1911 | 1915 | Toledo–Detroit Railroad | - | |
| Toledo, Ann Arbor and North Michigan Railway | AA | 1884 | 1895 | Ann Arbor Railroad | - | |
| Toledo Belt Railway | W&LE | 1883 | 1996 | Norfolk and Western Railway | - | |
| Toledo, Canada Southern and Detroit Railway | NYC | 1872 | 1916 | Michigan Central Railroad | - | |
| Toledo and Chicago Railroad | WAB | 1901 | 1901 | Wabash Railroad | - | |
| Toledo and Cincinnati Railroad | B&O | 1917 | 1944 | Baltimore and Ohio Railroad | - | |
| Toledo, Cincinnati and St. Louis Railroad | B&O, DT&I, NKP, PRR | 1882 | 1885 | Cincinnati, Lebanon and Northern Railway, Dayton and Ironton Railroad, Dayton, Lebanon and Cincinnati Railroad, Dayton and Toledo Railroad, Iron Railway, Toledo, Dupont and Western Railway | - | |
| Toledo, Columbus and Cincinnati Railway | NYC | 1889 | 1892 | Toledo and Ohio Central Railway | - | |
| Toledo, Columbus and Ohio River Railroad | PRR | 1911 | 1926 | Pennsylvania, Ohio and Detroit Railroad | - | |
| Toledo, Columbus and Southern Railway | NYC | 1885 | 1888 | Toledo, Columbus and Cincinnati Railway | - | |
| Toledo, Delphos and Burlington Railroad | B&O, DT&I, NKP | 1879 | 1882 | Toledo, Cincinnati and St. Louis Railroad | - | |
| Toledo, Delphos and Indianapolis Railway | B&O, NKP | 1872 | 1879 | Toledo, Delphos and Burlington Railroad | - | |
| Toledo–Detroit Railroad | DT&I | 1915 | 1931 | Detroit, Toledo and Ironton Railroad | - | |
| Toledo Dock and Warehouse Company | PRR | 1898 | 1903 | Manufacturers Railway | - | |
| Toledo, Dupont and Western Railway | NKP | 1886 | 1886 | Toledo, St. Louis and Kansas City Railroad | - | |
| Toledo, Findlay and Springfield Railway | B&O | 1889 | 1890 | Bowling Green Railroad | - | |
| Toledo and Grand Rapids Railroad | NKP | 1874 | 1881 | Toledo, Delphos and Burlington Railroad | - | |
| Toledo and Illinois Railroad | WAB | 1853 | 1856 | Toledo, Wabash and Western Railroad | - | |
| Toledo and Indianapolis Railway | NYC | 1881 | 1885 | Toledo, Columbus and Southern Railway | - | |
| Toledo and Maumee Narrow Gauge Railroad | NKP | 1873 | 1879 | Toledo, Delphos and Burlington Railroad | - | |
| Toledo and Michigan Railway | NYC | 1882 | 1883 | Michigan and Ohio Railroad | - | |
| Toledo and Michigan Belt Railway | NYC | 1887 | 1897 | Toledo, Canada Southern and Detroit Railway | - | |
| Toledo and Michigan Terminal Railroad | PRR | 1901 | 1903 | Manufacturers Railway | - | |
| Toledo and Northwestern Railroad | DT&I | 1904 | 1904 | Toledo, Ann Arbor and Detroit Railroad | - | |
| Toledo, Norwalk and Cleveland Railroad | NYC | 1850 | 1853 | Cleveland and Toledo Railroad | - | |
| Toledo and Ohio Central Railway | NYC | 1885 | 1952 | New York Central Railroad | - | |
| Toledo and Ohio Central Extension Railroad | - | |||||
| Toledo and Ohio Northern Railway | - | |||||
| Toledo and Ottawa Beach Railway | CN/ NKP | 1898 | 1899 | Pleasant Bay Railway | - | |
| Toledo Railway and Terminal Company | B&O/ CN/ C&O/ NKP/ NYC/ PM/ PRR | 1901 | 1907 | Toledo Terminal Railroad | - | |
| Toledo and Rossford Railroad | - | |||||
| Toledo, St. Louis and Kansas City Railroad | NKP | 1886 | 1900 | Toledo, St. Louis and Western Railroad | - | |
| Toledo, St. Louis and Western Railroad | NKP | 1900 | 1923 | New York, Chicago and St. Louis Railroad | - | |
| Toledo Southeastern Railway | - | |||||
| Toledo and State Line Railroad | AA | 1872 | 1878 | Toledo and Ann Arbor Railroad | - | |
| Toledo Terminal Railroad | TT | B&O/ CN/ C&O/ NKP/ NYC/ PM/ PRR | 1907 | 1989 | CSX Transportation | - |
| Toledo, Tiffin and Eastern Railroad | PRR | 1871 | 1876 | Northwestern Ohio Railway | - | |
| Toledo and Wabash Railroad | WAB | 1858 | 1858 | Toledo and Wabash Railway | - | |
| Toledo and Wabash Railway | WAB | 1858 | 1865 | Toledo, Wabash and Western Railway | - | |
| Toledo, Wabash and Western Railroad | WAB | 1856 | 1858 | Toledo and Wabash Railroad | - | |
| Toledo, Wabash and Western Railway | WAB | 1865 | 1876 | Wabash Railway | - | |
| Toledo, Walhonding Valley and Ohio Railroad | PRR | 1891 | 1911 | Toledo, Columbus and Ohio River Railroad | - | |
| Toledo Western Railroad | WAB | 1889 | 1889 | Wabash Railroad | - | |
| Toledo and Woodville Railroad | PRR | 1869 | 1878 | Northwestern Ohio Railway | - | |
| Trumbull and Mahoning Railroad | B&O | 1887 | 1915 | Baltimore and Ohio Railroad | - | |
| Union Depot Company | NYC/ PRR | 1872 | 1976 | Consolidated Rail Corporation | - | |
| Union Railroad | B&O | 1858 | 1860 | Marietta and Cincinnati Railroad | - | |
| United Terminal Railway | B&O | 1889 | 1895 | Marietta Railway | - | |
| Valley Railway | B&O | 1871 | 1895 | Cleveland Terminal and Valley Railway | - | |
| Van Wert, Paulding and Michigan Railway | NYC | 1874 | 1881 | Cincinnati, Van Wert and Michigan Railroad | - | |
| Vandalia Railroad | PRR | 1913 | 1917 | Pittsburgh, Cincinnati, Chicago and St. Louis Railroad | - | |
| Wabash Railroad | WAB | WAB | 1942 | 1991 | Norfolk and Western Railway | - |
| Wabash Railroad | WAB | 1889 | 1915 | Wabash Railway | - | |
| Wabash Railway | WAB | 1915 | 1942 | Wabash Railroad | - | |
| Wabash Railway | WAB | 1877 | 1879 | Wabash, St. Louis and Pacific Railway | - | |
| Wabash Railway of Ohio | WAB | 1876 | 1877 | Wabash Railway | - | |
| Wabash Pittsburgh Terminal Railway | WPT | P&WV | 1904 | 1916 | Pittsburgh and West Virginia Railway | - |
| Wabash, St. Louis and Pacific Railway | WAB | 1879 | 1886 | Detroit and State Line Wabash Railroad, Toledo Western Railroad | - | |
| Wabash Western Railway | WAB | 1889 | 1889 | Wabash Railroad | - | |
| Walhonding Valley Railway | PRR | 1889 | 1891 | Toledo, Walhonding Valley and Ohio Railroad | - | |
| Waynesburg and Canton Railroad | W&LE | 1888 | 1891 | Cleveland and Canton Railroad | - | |
| Waynesville, Port William and Jeffersonville Railroad | DT&I | 1875 | 1877 | Columbus, Washington and Cincinnati Railroad | - | |
| Wellston and Jackson Belt Railway | C&O | 1895 | 1930 | Chesapeake and Ohio Railway | - | |
| Wheeling Bridge and Terminal Railway | PRR | 1889 | 1900 | Wheeling Terminal Railway | - | |
| Wheeling and Cincinnati Mineral Railway | B&O | 1882 | 1883 | Cincinnati, Wheeling and New York Railroad | - | |
| Wheeling and Cleveland Railroad | NYC | 1895 | 1901 | Lake Erie, Alliance and Wheeling Railroad | - | |
| Wheeling and Harrisburg Railway of West Virginia | PRR | 1884 | 1889 | Wheeling Bridge and Terminal Railway | - | |
| Wheeling and Lake Erie Railroad | W&LE | 1899 | 1916 | Wheeling and Lake Erie Railway | - | |
| Wheeling and Lake Erie Railroad | W&LE | 1871 | 1886 | Wheeling and Lake Erie Railway | - | |
| Wheeling and Lake Erie Railway | W&LE, WLE | W&LE | 1916 | 1988 | Norfolk and Western Railway | - |
| Wheeling and Lake Erie Railway | W&LE | 1886 | 1899 | Wheeling and Lake Erie Railroad | - | |
| Wheeling and Lake Erie Bridge Company | W&LE | 1881 | 1887 | Wheeling and Lake Erie Railway | - | |
| Wheeling Terminal Railway | PRR | 1900 | 1954 | Penndel Company | - | |
| Wicks and Wells Railroad | ERIE | 1871 | 1882 | Youngstown and Austintown Railway | - | |
| Youngstown Railroad | ERIE | 1871 | 1882 | Youngstown and Austintown Railway | - | |
| Youngstown and Austintown Railway | ERIE | 1871 | 1941 | Erie Railroad | - | |
| Youngstown and Canfield Railroad | PRR | 1872 | 1873 | Lawrence Railroad | - | |
| Youngstown and Connotton Valley Railroad | W&LE | 1877 | 1879 | Connotton Valley Railroad | - | |
| Youngstown, Lawrence and Pittsburgh Railroad | PRR | 1887 | 1887 | Pittsburgh, Youngstown and Ashtabula Railroad | - | |
| Youngstown, New Castle and Pittsburgh Railroad | B&O | 1875 | 1875 | Pittsburgh and Northwestern Railroad | - | |
| Youngstown and Northern Railroad | YN | 1909 | 1981 | N/A | - | |
| Youngstown and Pittsburg Railroad | PLE | 1877 | 1878 | Pittsburgh and Lake Erie Railroad | - | |
| Youngstown and Ravenna Railroad | PRR | 1899 | 1954 | Penndel Company | - | |
| Youngstown and Salem Railroad | PLE/ PRR | 1903 | 1903 | Youngstown and Southern Railway | - | |
| Youngstown and Southern Railway | YS | PLE/ PRR | 1944 | 1996 | Railroad Ventures, Inc. | - |
| Youngstown and Southern Railway | PLE/ PRR | 1903 | 1916 | Youngstown and Suburban Railway | - | |
| Youngstown and Suburban Railway | YS | PLE/ PRR | 1916 | 1944 | Youngstown and Southern Railway | - |
| Youngstown and State Line Railroad | B&O | 1881 | 1881 | Pittsburgh and Western Railroad | - | |
| Zanesville Belt and Terminal Railway | W&LE | 1901 | 1948 | Wheeling and Lake Erie Railway | - | |
| Zanesville, McConnellsville and Pomeroy Railway | B&O | 1883 | 1885 | Chicago, Zanesville and Atlantic Railway | - | |
| Zanesville, Mount Vernon and Marion Railway | W&LE | 1886 | 1895 | Zanesville Belt and Terminal Railway | - | |
| Zanesville and Ohio River Railway | B&O | 1886 | 1900 | Ohio and Little Kanawha Railroad | - | |
| Zanesville and South Eastern Railway | PRR | 1881 | 1882 | Bellaire, Zanesville and Cincinnati Railway | - | |
| Zanesville Terminal Railroad | NYC/ PRR | 1902 | 1969 | Penndel Company | - | |
| Zanesville Terminal Railway | NYC/ PRR | 1890 | 1902 | Zanesville Terminal Railroad | - | |
| Zanesville and Western Railway | NYC | 1902 | 1938 | Toledo and Ohio Central Railway | - |
;Electric railways
- Akron, Bedford and Cleveland Railroad
- Akron and Cuyahoga Falls Rapid Transit Company
- Blissfield Railroad
- Canton and Massillon Electric Railway
- Cincinnati, Dayton and Toledo Traction
- Cincinnati, Hamilton and Dayton Railway
- Cincinnati and Lake Erie Railroad
- Cincinnati Street Railway
- Cleveland Railway
- Columbus, Delaware and Marion Railway
- Columbus, Marion and Bucyrus Railway
- Lake Shore Electric Railway
- Muskingum Electric Railroad
- Newark and Granville Electric Street Railway
- Ohio Electric Railway
- Sandusky, Milan and Norwalk Electric Railway
- Shaker Heights Rapid Transit
- Toledo and Indiana Railway
- Toledo, Port Clinton and Lakeside Railway
- Toledo and Western Railway
- Youngstown and Ohio River Railroad