Margaret Douglas, Countess of Bothwell was a Scottish aristocrat and courtier. She was a daughter of David Douglas, 7th Earl of Angus and Margaret Hamilton, daughter of John Hamilton of Samuelston, sometimes called "Clydesdale John", and a half-brother of Regent Arran. She was first married to Walter Scott of Branxholme & Buccleuch, who died in 1574. Their children included:
On 1 December 1577, she married Francis Stewart, 5th Earl of Bothwell. After a brief honeymoon, the new earl was not permitted to come within twenty miles of his new wife 'for reassone of his youngnes'. In June 1588 she entertained a party of English officers from Berwick at Coldingham including Captain Carey, the Provost Marshal, Captain Walker, George Barratt, James Somer. At the end of the dinner the Scottish gentleman toasted James VI and prayed for revenge for the death of his mother, Mary, Queen of Scots. One officer replied that it was good for James VI that she was gone, as she had been a usurper. Captain Carey came to another dinner at Coldingham and the Earl of Bothwell got him drunk, and he walked for a long time alone with countess, out of earshot. It was said she that questioned him on matters of state. At the end of this dinner, the same toast was made, and Captain Walker replied that Mary had been executed by law. Her daughter Elizabeth Stewart was baptised at Holyroodhouse on 1 March 1590. The English diplomat Robert Bowes gave a gift of a silver ewer and basin worth £100, made in Edinburgh by the silversmith Thomas Foulis, and Bowes also gave rewards to the nurse, midwives, musicians and servants in Bothwell's household. Bowes asked Francis Walsingham to make arrangements for credit in London for these diplomatic gifts. Elizabeth Stewart wife of James Stewart, 2nd Earl of Moray was witness for Queen Elizabeth. At the coronation of Anne of Denmark on 17 May 1590, the Countess of Bothwell, Robert Bowes's wife Eleanor Musgrove, the Countess of Mar, and Jean Kennedy, Countess of Orkney, carried the train of the queen's gown and cloak. The Earl of Bothwell entered Holyrood House in December 1591, and it was alleged that Margaret Douglas was waiting in a house in the Canongate for her husband's success, with jewels and money to attend the queen. In October 1592 she and Bothwell were in England, and were at the house of Walter Graham at Netherbyon the water of Esk. On 17 November 1592 she kneeled on the street before James VI as he was going toEdinburgh Castle, and after Lord Home and Lord Lindsay spoke in her favour she was allowed to kiss the king's hand, who spoke harshly of her and her husband. She was applauded and carried back to her lodging by well-wishers. She was heavily pregnant. James VI made a proclamation against her the next day for her support of her rebel husband. She was said to be "a griter mellair", to have had more involvement in her husband's treasons, "than became a woman". In July 1593 Bothwell entered Holyroodhouse house again to seek the king's forgiveness or to kidnap him, and it was said the countess had organised this by negotiating with Marie Ruthven, Countess of Atholl, who engaged the support of her husband, the Earl of Atholl, and the Duke of Lennox, Lord Ochiltree, and Lord Spynie. In September 1595 she got a letter in her favour from the king after "prostrating herself in his highness's way and presence." However she had the letter proclaimed publicly, not as intended, for it was meant only to allow her to dwell peaceably at Moss Tower. She died in 1640. Her children with Stewart included:
