Margaret is best remembered for having been a companion of Anne Boleyn, whose family estates lay near the Wyatts' and who later employed Margaret as one of her ladies-in-waiting. A portrait by Hans Holbein the Younger shows a woman presumed to be Margaret at the age of thirty-four, and it is assumed that it was painted around 1540. It is therefore probable that Margaret was close to Anne in age, being born around 1506. Few question that there was some form of friendship between Lady Margaret and Queen Anne. There is also a strong tradition which states that Margaret's sister, Mary, was also part of the Queen's social circle and that Margaret's brother, Thomas Wyatt, fell passionately in love with Anne in the 1520s. Another female favourite of the Queen's was Lady Bridget Wingfield, who died while giving birth in 1531. Margaret was one of Anne's chief ladies-in-waiting, and accompanied her to Calais, France in 1532, where it is presumed Anne and Henry VIII made secret plans to marry in the immediate future. It is known that Anne had a lady-in-waiting who "she loves as a sister", and it has been suggested that this lady was Margaret. She was certainly part of the Queen's circle of favourites. As Mistress of the Queen's Wardrobe, she would presumably have played a leading part in the decadent social life at court in the mid-1530s, which was fuelled by the extravagance of Henry and Anne. Lady Margaret was sent to attend her royal mistress in the Tower of London in May 1536 when the Queen was arrested on charges of adultery, treason and incest. Margaret also attended Anne on the scaffold on 19 May, and even received the last gift of a prayer book from her. After Anne was beheaded, Margaret acted as chief mourner at her small funeral. Anne had written a short farewell to Margaret inside the prayer book: A sketch by the famed court artist Hans Holbein has been identified as being a sketch of a Queen Anne Boleyn when she was pregnant, but current research suggests that it might have been one of the Wyatt sisters — either Margaret or her sister, Mary. Lady Margaret Lee is commemorated in Songs and Sonnets, also known as Tottel's Miscellany, a poetry anthology published by the law printer Richard Tottel in 1557. The book contains poems written by Lady Margaret's brother, Sir Thomas Wyatt, as well as poems by the Earl of Surrey, Nicholas Grimald and a number of anonymous poets. Grimald's funeral elegy, "An Epitaph of the Lady Margaret Lee," advises the reader, "Man, by a woman learn, this life what we may call," and praises her "blood, friendship, beauty, youth," and other qualities. Lady Margaret is one of several Tudor ladies memorialized in the volume.
Marriage and issue
About 6 July 1532 Margaret Wyatt married Sir Anthony Lee, by whom she had four sons and five daughters:
