Mark–Houwink equation The Mark–Houwink equation , also known as the Mark–Houwink–Sakurada equation or the Kuhn–Mark–Houwink–Sakurada equation or the Landau–Kuhn–Mark–Houwink–Sakurada equation gives a relation between intrinsic viscosity and molecular weight : From this equation the molecular weight of a polymer can be determined from data on the intrinsic viscosity and vice versa . The values of the Mark–Houwink parameters, and, depend on the particular polymer-solvent system. For solvents, a value of is indicative of a theta solvent . A value of is typical for good solvents. For most flexible polymers,. For semi-flexible polymers,. For polymers with an absolute rigid rod, such as Tobacco mosaic virus ,. It is named after Herman F. Mark and Roelof Houwink .Applications In size-exclusion chromatography , such as gel permeation chromatography , the intrinsic viscosity of a polymer is directly related to the elution volume of the polymer. Therefore, by running several monodisperse samples of polymer in a gel permeation chromatograph, the values of and can be determined graphically using a line of best fit . Then the molecular weight and intrinsic viscosity relationship is defined. Also, the molecular weights of two different polymers in a particular solvent can be related using the Mark–Houwink equation when the polymer-solvent systems have the same intrinsic viscosity:Knowing the Mark–Houwink parameters and the molecular weight of one of the polymers allows one to find the molecular weight of the other polymer using a GPC. The GPC sorts the polymer chains by volume and as intrinsic viscosity is related to the volume of the polymer chain , the GPC data is the same for the two different polymers. For example, if the GPC calibration curve is known for polystyrene in toluene , polyethylene in toluene can be run in a GPC and the molecular weight of polyethylene can be found according to the polystyrene calibration curve via the above equation.
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