Masters W40 400 metres world record progression
This is the progression of world record improvements of the 400 metres W40 division of Masters athletics.
;Key:
| Hand | Auto | Athlete | Nationality | Birthdate | Location | Date |
| 53.05 A | Maria Magnolia Figueiredo | 11.11.1963 | Bogotà | 11.07.2004 | ||
| 53.68 A | Sara Montecinos | 08.03.1954 | Cali | 20.03.1994 | ||
| 55.38 | Maeve Kyle | 06.10.1928 | Edinburgh | 22.07.1970 |