Mean corpuscular hemoglobin concentration


The mean corpuscular hemoglobin concentration is a measure of the concentration of haemoglobin in a given volume of packed red blood cell.
It is calculated by dividing the haemoglobin by the haematocrit. Reference ranges for blood tests are 32 to 36 g/dL, or between 4.81 and 5.58 mmol/L. It is thus a mass or molar concentration. Still, many instances measure MCHC in percentage, as if it were a mass fraction. Numerically, however, the MCHC in g/dL and the mass fraction of haemoglobin in red blood cells in % are identical, assuming an RBC density of 1g/mL and negligible haemoglobin in plasma.

Interpretation

A low MCHC can be interpreted as identifying decreased production of hemoglobin. MCHC can be normal even when hemoglobin production is decreased due to a calculation artifact. MCHC can be elevated in hereditary spherocytosis, sickle cell disease and homozygous haemoglobin C disease, depending upon the hemocytometer. MCHC can be elevated in some megaloblastic anemias. MCHC can be falsely elevated when there is agglutination of red cells or when there is opacifaction of the plasma. Causes of plasma opacification that can falsely increase the MCHC include hyperbilirubinemia, hypertryglyceridemia, and free hemoglobin in the plasma.

Complicating conditions

Because of the way automated analysers count blood cells, a very high MCHC may indicate the blood is from someone with a cold agglutinin, or there may be some other problem resulting in one or more artifactual results affecting the MCHC.
For example, for some patients with cold agglutinins, when their blood gets colder than 37 °C, the red cells will clump together. As a result, the analyzer may incorrectly report a low number of very dense red blood cells. This will result in an impossibly high number when the analyzer calculates the MCHC. This problem is usually picked up by the laboratory before the result is reported. The blood can be warmed until the cells separate from each other, and quickly put through the machine while still warm.
There are four steps to perform when a suspect increased MCHC is received from the analyzer:
  1. Remix the EDTA tube—if the MCHC corrects, report corrected results
  2. Incubation at 37 °C—if the MCHC corrects, report corrected results and comment on possible cold agglutinin
  3. Saline replacement: Replace plasma with same amount of saline to exclude interference e.g. Lipemia and Auto-immune antibodies—if the MCHC corrects, report corrected results and comment on Lipemia
  4. Check the slide for spherocytosis

    Worked example