Mycobacterium botniense

Description

• Gram-positive, nonmotile and acid-fast rods.

• Colonies on Löwenstein-Jensen media and on Middlebrook 7H11 agar are small, dysgonic and scotochromogenic, and produce yellow pigment.

• Visible growth from diluted inocula requires 5 to 8 weeks. Growth occurs at 37 to 50 °C.
• The type strain is positive for 10-d arylsulfatase and pyrazinamidase.
• Negative for 3-d arylsulfatase, urease, nitrate reductase, semi-quantitative catalase, heat-stable catalase, acid phosphatase, b-galactosidase and 5% NaCl tolerance.
• Tween 80 is not hydrolysed in 10 d.

• A phylogenetic tree based on the evaluation of 16S rDNA sequences places M. botniense among the slow-growing mycobacteria, closest to M. xenopi.

  Pathogenesis

• Not known, but first isolated from an environmental source.

  Type strain

• First isolated in Finland from stream waters. Strain E347 = ATCC 700701 = CCUG 47976 = CIP 106753 = DSM 44537.