Neolicaphrium was a proterotherid of small to medium size. N. recens, weighing about 37 kilograms was one third smaller thanN. major. In general terms, Neolicaphrium resembled Thoatherium of the Miocene, one of the most famous proterotherids, by its relatively graceful built adapted to a cursorial locomotion, although without presenting the extreme monodactyly that characterizes Thoatherium, so that still retained its three fingers in each hand and foot. Similar to the smaller deer of today, such as the pudus, the pampas deer and the muntiacus, Neolicaphrium was a browsing herbivore. The isotopic analysis of the fossils indicates that Neolicaphrium fed mainly on fruits and to a lesser extent on terrestrial plants that grew at ground level, and that leaves were only a very limited part of their diet. The composition of the fauna of the Sopas Formation in Uruguay, where fossils of N. recens from the late Pleistocene have been found, indicates that Neolicaphrium was a resident of savannahs and open tree forests. The rocks of the Sopas Formation were deposited in a gallery forest with rivers and Neolicaphrium lived there along with other mammals such as tapirs, the white-lipped peccary, the prehensile tail porcupine Coendou magnus, the capybara, the jaguar and the otter, species that characterize the tropical forest areas of South America.
Extinction
Previously it was thought that the family Proterotheriidae became extinct during the Pliocene, as a consequence of the climatic changes that occurred in the transition to the Pleistocene, along with the notoungulate pachyruchines and the argyrolagid marsupials. The fossil record of N. recens however, showed that this group survived until the late Pleistocene in forest areas, outside the typical Pampa regions of the Southern Cone that were predominant during the Quaternary ice ages; However, this idea was rejected until the 21st century, when the new fossil finds allowed to corroborate its presence in the Pleistocene. In the Sopas Formation have been found also fossils of several types of deer. Neolicaphrium therefore coexisted throughout the Pleistocene with ungulate mammals of holarctic origin. Both competition with these animals, which reached South America during the Great American Biotic Interchange, and the environmental changes occurring since the end of the Miocene, which led to the disappearance of forest areas, may have contributed the decline and extinction of proterotherids.