Nucleic acid methods

Purification

• Phenol–chloroform extraction
• Minicolumn purification
• RNA extraction
• Boom method
• Synchronous coefficient of drag alteration DNA purification

  Quantification

• Abundance in weight: spectroscopic nucleic acid quantitation
• Absolute abundance in number: real-time polymerase chain reaction
• High-throughput relative abundance: DNA microarray
• High-throughput absolute abundance: serial analysis of gene expression
• Size: gel electrophoresis

  Synthesis

• De novo: oligonucleotide synthesis
• Amplification: polymerase chain reaction

  Kinetics

• Multi-parametric surface plasmon resonance
• Dual-polarization interferometry
• Quartz crystal microbalance with dissipation monitoring

  Gene function

• RNA interference

  Other

• Bisulfite sequencing
• DNA sequencing
• Expression cloning
• Fluorescence in situ hybridization
• Lab-on-a-chip
• Comparison of nucleic acid simulation software
• Northern blot
• Nuclear run-on assay
• Radioactivity in the life sciences
• Southern blot
• Differential centrifugation
• Toeprinting assay
• Several bioinformatics methods, as seen in list of RNA structure prediction software