Palbociclib


Palbociclib, sold under the brand name Ibrance among others, is a medication developed by Pfizer for the treatment of HR-positive and HER2-negative breast cancer. It is a selective inhibitor of the cyclin-dependent kinases CDK4 and CDK6. Palbociclib was the first CDK4/6 inhibitor to be approved as a cancer therapy.

Mechanism of action

It is a selective inhibitor of the cyclin-dependent kinases CDK4 and CDK6.
In the G1 phase of the cell cycle, mammalian cells must pass a checkpoint, known as the restriction point "R", in order to complete the cell cycle and divide. CDK4 and CDK6 complex with cyclin D drive the phosphorylation of the retinoblastoma protein, Rb, which allows the cell to pass R and commit to division. Regulation of one or more proteins involved in this checkpoint is lost in many cancers. However, by inhibiting CDK4/6, palbociclib ensures that the cyclin D-CDK4/6 complex cannot aid in phosphorylating Rb. This prevents the cell from passing R and exiting G1, and in turn from proceeding through the cell cycle.

Administration

Palbociclib is taken daily orally with food in a cycle of 21 days of active medication followed by 7 without. Currently palbociclib is prescribed as a combination therapy with either letrozole or fulvestrant. Patients should also not consume CYP3A inhibitors or inducers while taking palbociclib. FDA information also cautions against consuming grapefruit products while taking palbociclib.

Approvals and indications

HR+ breast cancer

The drug was reviewed and approved under the Food and Drug Administration’s accelerated Priority Review and Breakthrough Therapy designation programs on February 3, 2015 as a treatment for patients with estrogen receptor positive advanced breast cancer. This was an accelerated approval.
In March 2017, the FDA granted regular approval to palbociclib for hormone receptor positive, human epidermal growth factor receptor 2 negative advanced or metastatic breast cancer, in combination with an aromatase inhibitor.
A phase III trial, PALOMA-2, was fully enrolled by February 2015 and reported positive results in April 2016. The results of PALOMA-2 trial showed significantly longer progression-free survival in patients on palbociclib in combination with letrozole, compared to patients on letrozole and placebo. Progression-free survival was assessed by radiologically confirmed disease progression by RECIST criteria or death during the study. At the time of publication, there was insufficient data on overall survival, and a final analysis is planned after a total of 390 deaths occur per protocol and in agreement with regulatory agencies. Of note, it was noted that the addition of palbociclib caused higher rates of myelotoxic events in the study.
The drug was approved for use in the European Union in November 2016 as a treatment for hormone receptor positive, human epidermal growth factor receptor 2 negative locally advanced or metastatic breast cancer either in combination with an aromatase inhibitor or, for women who have received prior endocrine therapy, in combination with fulvestrant. In pre- or perimenopausal women, a luteinizing hormone releasing hormone agonist should also be given.
In December 2017, palbociclib, was accepted for use by the NHS after going through the Scottish Medicines Consortium's process for medicines used to treat very rare and end-of-life breast cancer.

Adverse effects

A majority of patients taking palbociclib experience neutropenia, a condition where a patient has an abnormally low number of neutrophils. This side effect impacts the immune system, and is thus likely responsible for the second most common side effect, infection. Leukopenia and anemia are also frequent among patients taking palbociclib. More than 10% of patients also experience side effects such as fatigue, nausea, diarrhea, respiratory infection, headache, thrombocytopenia, vomiting, and decreased appetite. The FDA also indicates that patients should be vigilant to monitor themselves for any sign of pulmonary embolism. The FDA further cautions that women should be aware that the medication can have a harmful effect on a fetus, and thus should not be taken while pregnant.

Mechanisms of resistance

Resistance to palbociclib

FDA- and EMA-approval for palbociclib hinges upon the clinical trials’ results regarding progression-free survival. However, though data has proven promising, there is currently still no demonstrated significant improvement in overall survival in taking palbociclib. For example, in the PALOMA-2 trial, more than 70% of patients who were treated with palbociclib and letrozole progressed by 40 months. This has ultimately impeded the drug's overall uptake worldwide as health economic analyses have not found palbociclib to be a cost-effective drug.

''De novo'' resistance

Research has found de novo resistance against palbociclib to implicate a variety of targets both upstream and downstream of the CDK4/6 to Rb pathway. Studies have shown that overexpression of the transcription factor E2F2 is capable of promoting resistance to CDK4/6 inhibition more than just loss of Rb alone. It has also been noted that palbociclib is less effective in patients whose breast cancer cells overexpress cyclin E1 or E2, and Brk. Brk is an intracellular kinase that is overexpressed in 60% of breast cancers and whose amplification leads to increased phosphorylation of Y88 of the p27 protein, as well as increases the cyclin D and CDK4 activity, thereby rendering the cancer cells more resistant to palbociclib.
A recent study has also found that mutations in a pathway completely unrelated to the CDK4/6-Rb axis is also implicated in resistance to palbociclib. Loss of the FAT1 tumor suppressor was found to promote resistance to CDK4/6 inhibitors through the Hippo pathway, a signaling pathway known to function as a tumor suppressor. Previously, FAT1 has been noted as a putative tumor suppressor and oncogene, though never before explicitly linked to resistance. The team found that FAT1 suppressed cells required significantly higher doses of CDK4/6 inhibitors in order to block proliferation of breast cancer cells in vivo.

Resistance due to endocrine therapy

Palbociclib is currently indicated in HR+, HER2- metastatic breast cancer cells. As CDK4/6 inhibition acts directly downstream of the endocrine therapy targets, it reasons to argue that there perhaps could be cross-therapy resistance as a result of patient progression on hormone-therapy. In general, however, research has found that endocrine-resistant tumors are able to maintain sensitivity to CDK4/6 inhibition, with particular success witnessed with patients given combination therapy of palbociclib with endocrine therapy. For example, the PALOMA-3 trial which studied the benefit of palbociclib and fulvestrant vs. fulvestrant alone found that the former treatment regimen improved PFS in both patients with an ESR1 mutation and those with ESR1 wildtype ctDNA. Such results indicate that CDK4/6 inhibitors are effective irrespective of ESR1 mutation status, a marker that otherwise would mark a patient as endocrine-resistant.
Additionally, it is actually suggested that certain manifestations of endocrine therapy resistance may actually further sensitize the breast cancer to CDK4/6 inhibitors. For example, deficiencies in mismatch repair caused by the MutL mutation in ER+ breast cancer evades CHK2-mediated inhibition of CDK4, thereby leading to endocrine resistance. CDK4/6 inhibitors, however, have been demonstrated to be highly effective in MutL-defective ER+ breast cancer cells, and MutL bears potential as a biomarker for identifying patients highly suitable for treatment with CDK4/6 inhibitors.

Other mechanisms of resistance

Interestingly enough, researchers at the Dana Farber Institute found that breast cancer cells that developed resistance to palbociclib were able to become resensitized to the drug following a seven-day "treatment holiday". Scientists linked the initial development of resistance to an increase in expression of CDK6, with the specific mechanism of CDK6 upregulation originating from suppression of the TGF-β pathway via the miR-432-5p microRNA. Scientists made this discovery after noticing that, in the lab, all breast cancer cells in the dish were found to acquire resistance at a similar time, a phenomenon contrary to classical models of acquiring resistance in which one or two cells become resistant and then expand to encompass more of the tumor as they divide. These palbociclib-resistance cells were also noted to not contain a particular mutation, but rather became resistant via and continued to spread this resistance to neighboring cells via exosomes. While the specific mechanism behind this resistance has not yet been elucidated, it does open an avenue for further research into a completely novel method through which cancer cells acquire resistance. On the other hand, this also then suggests that perhaps patients which seemingly progress on palbociclib could perhaps benefit from longer-term treatment following a shorter treatment holiday in which their tumors are "reset".
There are a variety of means through which cancer cells become resistant to palbociclib, with these mechanisms of resistance involving multiple targets and processes throughout the pathway that palbociclib operates on. Previous research estimates that breast cancer cells show adaptation against palbociclib as early as 72 hours post-treatment. Evidence for this was "shown to be mediated by non-canonical activation of cyclin D1/CDK2 complexes, which in turn induced a recovery of cyclin E2 expression and S-phase entry despite CDK4/6 inhibition" upstream of these two targets. Furthermore, it is estimated that approximately 10% of patients will have primary resistance to CDK4/6 inhibitors. For example, patients who display evidence of functional Rb loss at baseline are not likely to benefit from CDK4/6 inhibition, nor are patients who display baseline evidence of increased cyclin E1 expression, or a high CCNE1/RB ratio.

Clinical trials

HR+ breast cancer

The PALOMA-3 trial announced in April 2015 that the addition of palbociclib was superior to fulvestrant alone for progression-free survival.
In the phase II PALOMA-1 trial reported at the April 2014 annual meeting of the American Association for Cancer Research, the addition of palbociclib to letrozole was shown to significantly slow the progression of advanced cancer, but was not shown to have a statistically significant effect on increasing patients' overall survival times.
Pfizer announce on May 31, 2020 the results of a preplanned efficacy and futility analysis. The results indicate that, when used in combination with post-surgery endocrine therapy, it "is unlikely to show a statistically significant improvement in the primary endpoint of invasive disease-free survival." The trial was conducted on male and female patients with HR-positive, HER2-negative early stage breast cancer.

Active clinical trials

According to the NIH National Cancer Institute there are currently 39 active clinical trials testing palbociclib on its own or in combination with other medications. While a majority of these are exploring the further uses of palbociclib to treat breast cancer, other trials are investigating the potential applications of palbociclib to head and neck cancers, non-small cell lung cancer, recurring brain metastasis, squamous cell carcinoma, central nervous system tumors, and other solid tumor types.
In December 2017, Pfizer announced that the PALOMA-2 trial, an ongoing phase III trial combining palbociclib with letrozole, showed a 44% reduction in risk of disease progression among trial subjects. The trial has also demonstrated greater than a year's improved median progression free survival for patients on the combined therapy. PALOMA-2 median patient follow-up time now exceeds three years, making it the longest traceable data for phase III study of a CDK4/6 inhibitor.

Pricing

Ibrance "can be ordered through select" specialty pharmacies and "sells for $9,850 for 30 days or $118,200 for a year's supply before discounts." According to a statement by the New York–based Pfizer the price "is not the cost that most patients or payors pay" since most prescriptions are dispensed through health plans, which negotiate discounts for medicines or get government-mandated price concessions. In the United States specialty pharmacies fill prescriptions for drugs that are usually high cost.

Resistance to cost

In February 2017, the National Institute for Health and Care Excellence in the United Kingdom published a statement stating that the cost of Ibrance did not make the added health benefits worth the cost. Though the committee acknowledged Ibrance's ability to extend patient life by approximately 10 months, it was stated that with the side effects caused by Ibrance, it was not a cost-effective medication for NICE to endorse. At the time, a year's treatment with palbociclib and a drug such as fulvestrant was priced at US$106,105. In November 2017 NICE announced that, after negotiation with Pfizer, the price would be discounted, and the drug would be recommended for use.

Drugs with a similar mechanism of action

Palbociclib has several direct competitors currently on the market or in clinical trials. In September 2017, abemaciclib, another selective CDK4/6 inhibitor owned and manufactured by Eli Lilly, was approved for HR-positive, HER2-negative advanced metastatic breast cancer both in combination with fulvestrant and as a monotherapy. In March 2017, the FDA also approved ribociclib, owned by Novartis, as a combination therapy with aromatase inhibitors for indications similar to those of palbociclib and ribociclib. Notably, ribociclib seems to also have an inhibitory effect on Cyclin D3/CDK6 activity. G1 Therapeutics also has a Cdk4/6 inhibitor, trilaciclib, which is currently in phase II trials, but as of December 2017 has not gained FDA approval.

Brand names

In Bangladesh it is under the trade name Palbonix.