Panna National Park was declared as one of the Tiger reserves of India in 1994/95 and placed under the protection of Project Tiger. The decline of tiger population in Panna has been reported several times. Two female tigers were relocated there from Bandhavgarh National Park and Kanha National Park in March 2009. However, the last male tiger had already disappeared. A committee to look into the disappearance of the tigers was formed. In June 2009, it was officially announced that the Reserve, which had over 40 tigers six years earlier, had no tigers left and only two tigresses, which were brought in a little earlier In February 2012, only three years later, the entire tiger population of the reserve was considered eliminated. The Madhya Pradesh government did not determine responsibility for the debacle, nor did it pass the inquiry to the Central Bureau of Investigation in spite of requests from the Ministry of Environment and Forests and the Prime Minister's Office. The Ministry of Environment and Forests approved a proposal to translocate two tigers and two tigresses to the reserve. One female each from Bandhavgarh National Park and Kanha National Park were translocated to Panna Tiger Reserve. A tiger male, coded T3, was brought from Pench Tiger Reserve but strayed out of the park shortly thereafter, in November 2009 The tiger started walking towards its home in Pench National Park, indicating homing instinct. It moved steadily through human dominated landscape without causing any conflict. Forest department staff tracked it continuously for over a month and finally brought it back to the Panna Tiger Reserve. It then settled well, established territory and started mating. The tigress, T1, translocated from Bandhavgarh National Park, gave birth to four cubs in April 2010 of which 2 survive till date. The second tigress, T2, translocated from Kanha National Park gave birth to four cubs several months later and all four survive till date. A third tigress, coded T4, an orphaned cub was reintroduced to Panna in March 2011. She learnt hunting skills with the help of the male and mated with him. She was found dead on 19 September 2014 of an infection caused by its radio collar. Her sister T5 was released in Panna in November 2011. Thus four tigers and around 10 cubs of up to 2 years are settled in Panna Tiger Reserve at present and their progress is being regularly monitored by the Forest Department.
The Government of India along with Government of Madhya Pradesh and Government of Uttar Pradesh have planned to link the Ken River with Betwa River. This involves construction of 283m long Daudhan Dam. The project aims to transfer excess water from Ken basin to Betwa basin thus provide water access to the drought prone region of Bundelkhand. This construction will cause inundation of 400 hectares of land of 4300 hectare Panna Tiger reserve. Environmentalist are afraid that this will adversely affect the population of tigers in the region.