Pietro Biginelli was an Italian chemist, who discovered a three-component reaction between urea, acetoacetic ester and aldehydes. He also studied various aspects of sanitation chemistry and chemical products' quality control.
Biginelli's first known scientific work, in which he was a co-author to his mentor Icilio Guareschi, was focused on synthesis and reactivity of chlorobromonaphtalene. Already in the University of Florence, Biginelli described a three-component reaction between urea, aldehyde, and ethyl acetoacetate, which was at first incorrectly interpreted as one leading to the formation of alpha-benzuramido-crotonacetic ester, or ethyl-alpha-salicyluramido-crotonate with open-chain acyclic structures. However, later he corrected himself and expanded his initial studies, showing that the end products actually were pyrimidines. However, Biginelli did not change the chemical names presented earlier. Another scope of his research interests emerged when he was an assistant to Bartolomeo Gosio. At that time, it was known that certain poisonous volatile arsenic species tend to form at molds growing on wallpapers painted by arsenic-containing paints. Finally Gosio and Biginelli succeeded in isolating and analyzing the species: when Gosio gas was passed into a solution of mercuric chloride in dilute HCl a crystalline precipitate was formed. A small vial of the Gosio/Biginelli mercurichloride is to this day preserved at The Museum of the History of Medicine in Rome, Italy. Under the printed name “Laboratorio Batteriologico della Sanità Pubblica” is handwritten, “arsina penicillare comp. mercurico”. From analysis of this material and of the gas itself it appeared that the gas was diethylarsine. Later, however, it was found that gas discovered by Gosio and Biginelli was in fact trimethylarsine. Biginelli stated in 1911 that tannin has the formula C41H32O25 and that it was probably a glucoside. These conclusions were based on the property shown by tannin of forming additive products with water, alcohol, and ether, and also on the loss of carbon dioxide and water with formation of hexahydroxybenzophenone, when tannin is heated in aqueous solution with lead dioxide. In 1914, Biginelli showed that aristoquinine and quinine carbonate placed in market by Bayer and Zimmer in 1898 were not the salts of carbonic acid, that true quinine carbonate is very bitter, that their action on the organism is slight and slow compared with that of quinine, and that euquinine is ethyl quinine carboxylate, C20H23O2N2.COOC2H5, and aristochin, carbonylquinine, 2CO. After leaving his post as a director of Chemical Laboratory of State Medicine, Biginelli, as he himself stated, focused mainly on the problems of chemical commodity research, e.g. distinguishing between true and false tannates of commercial quinine, artificial tannins, etc.