Potato dextrose agar
Potato dextrose agar and potato dextrose broth are common microbiological growth media made from potato infusion, and dextrose. Potato dextrose agar is the most widely used medium for growing fungi and bacteria.Potato infusion can be made by boiling of sliced potatoes in ~ distilled water for 30 minutes and then decanting or straining the broth through cheesecloth. Distilled water is added such that the total volume of the suspension is. dextrose and agar powder is then added and the medium is sterilized by autoclaving at for 15 minutes.
A similar growth medium, Potato dextrose broth is formulated identically to PDA, omitting the agar. Common organisms that can be cultured on PDB are yeasts such as Candida albicans and Saccharomyces cerevisiae and molds such as Aspergillus niger.
