Reoviridae


Reoviridae is a family of viruses. They have a wide host range, including vertebrates, invertebrates, plants, protists and fungi. They lack lipid envelopes and package their genomes of discrete double-stranded segments of RNA within multi-layered capsids. Lack of a lipid envelope has allowed three-dimensional structures of these large complex viruses to be obtained, revealing a structural and likely evolutionary relationship to the Cystovirus family of Bacteriophage. There are currently 97 species in this family, divided among 15 genera in two subfamilies. Reoviruses can affect the gastrointestinal system and respiratory tract. The name "Reo-" is derived from respiratory enteric orphan viruses. The term "orphan virus" refers to the fact that some of these viruses have been observed not associated with any known disease. Even though viruses in the family Reoviridae have more recently been identified with various diseases, the original name is still used.
Reovirus infection occurs often in humans, but most cases are mild or subclinical. Rotavirus, however, can cause severe diarrhea and intestinal distress in children, and lab studies in mice have implicated Orthoreovirus in the expression of coeliac disease in pre-disposed individuals. The virus can be readily detected in feces, and may also be recovered from pharyngeal or nasal secretions, urine, cerebrospinal fluid, and blood. Despite the ease of finding Reovirus in clinical specimens, their role in human disease or treatment is still uncertain.
Some viruses of this family, such as Phytoreovirus and Oryzavirus, infect plants. Most of the plant-infecting reoviruses are transmitted between plants by insect vectors. The viruses replicate in both the plant and the insect, generally causing disease in the plant, but little or no harm to the infected insect.

Structure

Reoviruses are non-enveloped and have an icosahedral capsid composed of an outer and inner protein shell. Ultrastructural studies shows that virion capsids composed of two or tree separate layers which depend their type. The innermost layer has T=1 icosahedral symmetry and composed of 60 different type of structural proteins. The core contains genom segments, each of them encode variety enzyme structure which is required for transcription. The core is covered by capsid layer T=13 icosahedral symmetry. Reovirus has unique structure which is contain glycolisated spike protein on the surface. The genomes of viruses in Reoviridae contain 10–12 segments which are grouped into three categories corresponding to their size: L, M and S. Segments range from about 0.2 to 3 kbp and each segment encodes 1–3 proteins. Proteins of viruses in the Reoviridae are denoted by the Greek character corresponding to the segment it was translated from.
GenusStructureSymmetryCapsidGenomic arrangementGenomic segmentation
AquareovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
RotavirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
SeadornavirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
ColtivirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
FijivirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
PhytoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
MimoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
OrbivirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
CypovirusIcosahedralT=2Non-envelopedLinearSegmented
OrthoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
IdnoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
DinovernavirusIcosahedralT=2Non-envelopedLinearSegmented
OryzavirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
CardoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented
MycoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented

Life cycle

Viruses in the family Reoviridae have genomes consisting of segmented, double-stranded RNA. Because of this, replication occurs exclusively in the cytoplasm and the virus encodes several proteins which are needed for replication and conversion of the dsRNA genome into -RNAs. The virus can enter the host cell via a receptor on the cell surface. The receptor is not known but is thought to include sialic acid and junctional adhesion molecules. The virus is partially uncoated by proteases in the endolysosome, where the capsid is partially digested to allow further cell entry. The core particle then enters the cytoplasm by a yet unknown process where the genome is transcribed conservatively causing an excess of sense strands, which are used as mRNA templates to synthesize sense strands. The genome of rotavirus is separated via 11 segmented as mentioned before. These 11 segmented genome associated with VP1 molecule which responsible for RNA synthesizes. In early events the selection process is occurred to allow the entry of 11 different RNA species in the cell. This procedure is performed by newly synthesized RNAs. This event ensures to receives one each of the 11 different RNA species.
In late events transcription process is occurred again but this time are not capped unlikely early events. For virus different amounts of RNAs are required therefore in translation step there is a control machinery. There are same quantities RNAs but different quantities of proteins. The reason of this, RNAs are not translated same rate.
Viral particles begin to assemble in the cytoplasm 6–7 hours after infection. Translation takes place by leaky scanning, suppression of termination, and ribosomal skipping. The virus exits the host cell by monopartite non-tubule guided viral movement, cell to cell movement, and existing in occlusion bodies after cell death and remaining infectious until finding another host.
GenusHost detailsTissue tropismEntry detailsRelease detailsReplication siteAssembly siteTransmission
AquareovirusAquatic vertebrates: fish; aquatic invertebrates: shellfish; aquatic invertebrates: crustaceans-Cell receptor endocytosisCell deathCytoplasmCytoplasmPassive diffusion
CardoreovirusCrustaceans: crabs-Cell receptor endocytosisCell deathCytoplasmCytoplasmArthropod bite
ColtivirusHumans; rodents; ticks; mosquitoesErythrocytesCell receptor endocytosisCell deathCytoplasmCytoplasmArthropod bite
CypovirusInsectsMidgut; goblet; fatCell receptor endocytosisCell deathCytoplasmCytoplasmPolyhedra: oral-fecal; vertical: eggs
DinovernavirusInsects; Mosquitoes-UnknownCell deathCytoplasmCytoplasmUnknown
FijivirusPlants: gramineae; plants: liliacea; planthoppersPhloemViral movement; mechanical inoculationCell deathCytoplasmCytoplasmDelphacid plant hoppers
IdnoreovirusHymenopteraGutCell receptor endocytosisCell deathCytoplasmCytoplasmUnknown
MimoreovirusAlgae-Cell receptor endocytosisCell deathCytoplasmCytoplasmArthropod bite
MycoreovirusFungiMyceliumCell death; cytoplasmic exchange, sporogenesis; hyphal anastomosisCell death; cytoplasmic exchange, sporogenesis; hyphal anastomosisCytoplasmCytoplasmCytoplasmic exchange, sporogenesis; hyphal anastomosis
OrbivirusVertebrates; mosquitoes; midges; gnats; sandflies; ticks-Cell receptor endocytosisCell deathCytoplasmCytoplasmArthropod bite
OrthoreovirusVertebratesEpithelium: intestinal; epithelium:bile duct; epithelium: lung; leukocytes; endothelium: CNSClathrin-mediated endocytosisCell deathCytoplasmCytoplasmAerosol; oral-fecal
OryzavirusPlants: graminae, Oryza sativa; planthoppers-Viral movement; mechanicalCell deathCytoplasmCytoplasmDelphacid planthoppers
PhytoreovirusOryza sativa; leafhoppersPhloemViral movement; mechanical inoculationCell deathCytoplasmCytoplasmLeafhoppers
RotavirusHumans; vertebratesIntestinal mucosaClathrin-mediated endocytosisCell deathCytoplasmCytoplasmOral-fecal
SeadornavirusHumans; cattle; pigs; mosquitoes-Cell receptor endocytosisCell deathCytoplasmCytoplasmZoonosis; arthropod bite

Multiplicity reactivation

is the process by which two or more virus genomes, each containing inactivating genome damage, can interact within an infected cell to form a viable virus genome. McClain and Spendlove demonstrated MR for three types of reovirus after exposure to ultraviolet irradiation. In their experiments, reovirus particles were exposed to doses of UV-light that would be lethal in single infections. However, when two or more inactivated viruses were allowed to infect individual host cells MR occurred and viable progeny were produced. As they stated, multiplicity reactivation by definition involves some type of repair. Michod et al. reviewed numerous examples of MR in different viruses, and suggested that MR is a common form of sexual interaction in viruses that provides the benefit of recombinational repair of genome damages.

Taxonomy

Group: dsRNA
The Reoviridae are divided into two subfamilies based on the presence of a "turret" protein on the inner capsid. From ICTV communications: "The name Spinareovirinae will be used to identify the subfamily containing the spiked or turreted viruses and is derived from 'reovirus' and the Latin word 'spina' as a prefix, which means spike, denoting the presence of spikes or turrets on the surface of the core particles. The term 'spiked' is an alternative to 'turreted', that was used in early research to describe the structure of the particle, particularly with the cypoviruses. The name Sedoreovirinae will be used to identify the subfamily containing the non-turreted virus genera and is derived from 'reovirus' and the Latin word 'sedo', which means smooth, denoting the absence of spikes or turrets from the core particles of these viruses, which have a relatively smooth morphology."

Other Reoviridae

Additionally, there is one proposed genus that has not yet been accepted by the ICTV:
Piscine reovirus is a Spinareovirinae-like virus that has not yet been classified.

Therapeutic applications

Although reoviruses are mostly nonpathogenic in humans, these viruses have served as very productive experimental models for studies of viral pathogenesis. Newborn mice are exquisitely sensitive to reovirus infection and have been used as the preferred experimental system for studies of reovirus pathogenesis.
The reoviruses have been demonstrated to have oncolytic properties, encouraging the development of reovirus-based therapies for cancer treatment.
Reolysin is a formulation of reovirus that is currently in clinical trials for the treatment of various cancers, including studies currently developed to investigate the role of Reolysin combined with other immunotherapies.