After dropping off her son Evan with her ex-husband Cliff, Renee Morgan is abducted during a staged roadside tire repair by a five-person team overseen by Dr. Nyman. Stunned with a stun gun, Renee is carried into the team's truck, chained down and transported to a warehouse laboratory where she is strapped to a table and held prisoner in a private room. A fellow captive named Seth tells Renee to remember "G10 12X." Fellow captive Blake talks to Renee through the vent from an adjacent room and explains that their captors are forcing subjects to face their personal fears. Renee additionally learns that this mysterious group has secretly monitored subjects all over the world for decades. With the entire operation overseen by Terrence, Renee is repeatedly tested and experimented on by Dr. Nyman, Dr. Raxlen, Dianne, Colette, and a Bald Man. Suffering from arachnophobia, Renee panics when Dianne and the Bald Man test Renee by having a spider crawl on her. Renee eventually breaks free of her restraints and crawls into the ventilation system. Renee peeks into other holding cells and sees other captives being tested according to their fears. Renee makes her way to Blake’s room and hides under his table when the doctors enter. Exploiting Blake’s fear of snakes, the doctors cryptically reveal that they are using terror to initiate a DNA change in test subjects. Having failed his final test, Colette gives Blake a medication via a syringe, telling him it will sedate him and when he awakes, he will be back home. When Blake is wheeled out of his room, Renee uses the opportunity to sneak into the hallway through his open cell door and explore the lab. Realizing Renee may be their only hope, the team agrees to put Renee through one more test. Renee quickly crawls back through the ventilation system and returns to her room before it is discovered she is missing. Dianne, lying to Renee, seductively touches Renee under her clothing and tells her about Evan, explaining that she can learn details about Renee's son through Renee’s skin. Dianne additionally explains that G10 12X is a genetic code that all humans have in their chromosomes. By rupturing that part of their genes through terror, humans can change shape and evolve as a life form. Dianne morphs her head into a deformed shape to prove the truth to Renee, unaware that Renee has secretly taken the syringe originally intended for her. Renee stabs Dianne with the syringe and escapes back into the main facility. She finds a lab filled with DNA samples collected from numerous test subjects. She also overhears Dr. Raxlen and the Bald Man discussing the development of her ovum to accept non-human sperm. The Bald Man senses that Renee has escaped and instead of chasing her through the factory, he goes to a computer and uses it to locate Renee's location. He traps Renee in a room and uses gas to knock her unconscious. When Renee wakes, Terrence, Dr. Nyman, Dianne, and the Bald Man demonstrate that Renee now has improved eyesight and hearing. Terrence explains that they were all humans once, but overcoming fear transformed them. They now believe they can cure humanity of its self-destructive tendencies by building their numbers and altering the gene pool. Renee is forcibly strapped into a transparent helmet in which spiders are released to crawl all over her face. Renee frantically tears at the mask and hits her head against the wall while the doctors observe. Suddenly, Renee is no longer afraid. She "ruptures" and the shape of her head grotesquely distorts, causing the mask to crack away from her head. Renee returns to her everyday life as a covert plant for the group. Renee tries convincing her son that she knows people who can help him overcome his focus issues, but Evan refuses. Terrence, the Bald Man, and another henchman arrive at the house to take Evan by force. Renee has a change of heart and warns Evan to escape, which he does. Terrence calls off the chase and tells Renee that she will help him find Evan. Terrence also tells her that they have been waiting for Renee for a long time and now they finally have her.
The film holds a 18% positive rating at the film review aggregator website Rotten Tomatoes, based on 34 reviews, with an average rating of 4.39/10. The site's critical consensus reads, "Derivative and clumsily assembled, Rupture manages the tricky feat of making arachnophobia seem dull."
