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SSC buffer
In
biochemistry
and
molecular biology
, the
saline-sodium
citrate
buffer
is used as a
hybridization
buffer, to control stringency for
washing
steps
in
protocols
for
Southern blotting
,
in situ hybridization
,
DNA Microarray
or
Northern blotting
. 20X
SSC
may be used to
prevent
drying
of
agarose
gels
during a
vacuum
transfer.
A 20X
stock solution
consists of 3
M
sodium chloride
and
300
mM
trisodium citrate
.