Tsukuba made her professional debut as a manga artist by winning the 'Outstanding Work' award in the 5th LaLa Mangaka Grand Prix held in 1994 for her work,. Her winning work was later published in the July 10th issue of LaLa DX in the same year. After Hikari no Dokeki Haru no Hi ni, she published a one-shot titled, in the October issue of LaLa in the same year. Her next work, was published in the March 10th issue of Lunatic LaLa, one of LaLa's special issue in 1995. Mermaid Moon was Tsukuba's next one-shot was published in the January 10th issue of LaLa DX in 1996. Four months later, was published in the May 10th issue of the same magazine that published Mermaid Moon. It was later compiled into the second volume of her first series, Mekakushi no Kuni. Another one-shot, was published in the November 10th issue of LaLa DX. This was compiled into the first volume of Mekakushi no Kuni. In 1997, Tsukuba published 3 one-shots with the first titled as which was published in the July 10th issue of LaLa DX. Right after Bunny Game, another one-shot titled was published and compiled into the first volume of Mekakushi no Kuni. It was followed by in the November 10th issue of LaLa DX. and was published in the September issue of LaLa DX and the November issue of Melody in 1998 respectively. Ame no Naka was also compiled into the third volume of Mekakushi no Kuni. Her first series, started serializing in the January issue of LaLa DX in 1999. The serialization continued in the same magazine for seven chapters, including one special chapter which was published in March issue of LaLa. Later the series was moved and continued in the August issue of LaLa in 2000 beginning from its eighth chapter. During the course of the serialization of Mekakushi no Kuni, three separate one-shots were serialized. They were which was published in the March issue of Melody in 2000 and which was published in the July issue of LaLa in 2002 and was published in the May issue of a LaLa-Melody special collaborative issue, The LaLa×Melody. It was compiled into the second volume of Tsukuba's second series, Yoroshiku Master. For 2004, she came up with another new series titled in the January issue of LaLa DX just as Mekakushi no Kuni ends in January issue of LaLa in 2004 with 40 chapters without including several special chapters. The series has 9 collected volumes in tankōbon format with 5 volumes in bunkōban format. After the end of Mekakushi no Kuni, Tsukuba published another one-shot titled, in the May issue of LaLa before continuing Yoroshiku Master. Amai Kami Ato was also compiled into the first volume of Yoroshiku Master. In the October issue of LaLa, she started another new series titled,. It ended in the January issue of LaLa in 2008, with 35 chapters without including a special chapter which was serialized in the November issue of LaLa DX in 2007. The chapter was compiled into the third volume of Yoroshiku Master. The series was compiled into 7 volumes in tankōbon format. Yoroshiku Master is still ongoing as of October 2009, with 13 chapters compiled into 3 volumes. However, no new chapters were serialized since the January issue of LaLa DX. Another two one-shots, was published in the June issue of LaLa, while was published in the September issue of LaLa DX. For 2009, another one-shot, was published in the January issue of LaLa as well as making her official Melody debut by publishing a one-shot titled in the October issue.