Saliva
Saliva is an extracellular fluid produced and secreted by salivary glands in the mouth. In humans, saliva is 99.5% water plus electrolytes, mucus, white blood cells, epithelial cells, enzymes, antimicrobial agents such as secretory IgA, and lysozymes.
The enzymes found in saliva are essential in beginning the process of digestion of dietary starches and fats. These enzymes also play a role in breaking down food particles entrapped within dental crevices, thus protecting teeth from bacterial decay. Saliva also performs a lubricating function, wetting food and permitting the initiation of swallowing, and protecting the oral mucosa from drying out.
Various animal species have special uses for saliva that go beyond predigestion. Some swifts use their gummy saliva to build nests. Aerodramus nests form the basis of bird's nest soup.
Cobras, vipers, and certain other members of the venom clade hunt with venomous saliva injected by fangs. Some caterpillars produce silk fiber from silk proteins stored in modified salivary glands.
Composition
Produced in salivary glands, human saliva comprises 99.5% water, but also contains many important substances, including electrolytes, mucus, antibacterial compounds and various enzymes.- Water: 99.49%
- Electrolytes:
- * 2–21 mmol/L sodium
- * 10–36 mmol/L potassium
- * 1.2–2.8 mmol/L calcium
- * 0.08–0.5 mmol/L magnesium
- * 5–40 mmol/L chloride
- * 25 mmol/L bicarbonate
- * 1.4–39 mmol/L phosphate
- * Iodine
- Mucus
- Antibacterial compounds
- Epidermal growth factor
- Various enzymes; most notably:
- * α-amylase, or ptyalin, secreted by the acinar cells of the parotid and submandibular glands, starts the digestion of starch before the food is even swallowed; it has a pH optimum of 7.4
- * Lingual lipase, which is secreted by the acinar cells of the sublingual gland; has a pH optimum around 4.0 so it is not activated until entering the acidic environment of the stomach
- *Kallikrein, an enzyme that proteolytically cleaves high-molecular-weight kininogen to produce bradykinin, which is a vasodilator; it is secreted by the acinar cells of all three major salivary glands
- * Antimicrobial enzymes that kill bacteria:
- **Lysozyme
- **Salivary lactoperoxidase
- **Lactoferrin
- **Immunoglobulin A
- *Proline-rich proteins
- * Minor enzymes including: salivary acid phosphatases A+B, N-acetylmuramoyl-L-alanine amidase, NADH dehydrogenase, superoxide dismutase, glutathione transferase, class 3 aldehyde dehydrogenase, glucose-6-phosphate isomerase, and tissue kallikrein
- Cells: possibly as many as 8 million human and 500 million bacterial cells per mL. The presence of bacterial products causes saliva to sometimes exhibit a foul odor.
- Opiorphin, a pain-killing substance found in human saliva
- Haptocorrin, a protein which binds to Vitamin B12 to protect it against degradation in the stomach, before it binds to intrinsic factor
Daily salivary output
In humans, the submandibular gland contributes around 70–75% of secretion, while the parotid gland secretes about 20–25% and small amounts are secreted from the other salivary glands.
Functions
Saliva contributes to the digestion of food and to the maintenance of oral hygiene. Without normal salivary function the frequency of dental caries, gum disease, and other oral problems increases significantly.Lubricant
Saliva coats the oral mucosa mechanically protecting it from trauma during eating, swallowing, and speaking. Mouth soreness is very common in people with reduced saliva and food sticks to the inside of the mouth.Digestion
The digestive functions of saliva include moistening food and helping to create a food bolus. The lubricative function of saliva allows the food bolus to be passed easily from the mouth into the esophagus. Saliva contains the enzyme amylase, also called ptyalin, which is capable of breaking down starch into simpler sugars such as maltose and dextrin that can be further broken down in the small intestine. About 30% of starch digestion takes place in the mouth cavity. Salivary glands also secrete salivary lipase to begin fat digestion. Salivary lipase plays a large role in fat digestion in newborn infants as their pancreatic lipase still needs some time to develop.Role in taste
Saliva is very important in the sense of taste. It is the liquid medium in which chemicals are carried to taste receptor cells. Persons with little saliva often complain of dysgeusia. A rare condition identified to affect taste is that of , or excessive amounts of sodium in saliva that is not caused by any other condition, causing everything to taste 'salty'.Other
- Saliva maintains the pH of the mouth. Saliva is supersaturated with various ions. Certain salivary proteins prevent precipitation, which would form salts. These ions act as a buffer, keeping the acidity of the mouth within a certain range, typically pH 6.2–7.4. This prevents minerals in the dental hard tissues from dissolving.
- Saliva secretes carbonic anhydrase, which is thought to play a role in the development of taste buds.
- Saliva contains EGF. EGF results in cellular proliferation, differentiation, and survival. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
Production
The saliva stimulated by sympathetic innervation is thicker, and saliva stimulated parasympathetically is more fluid-like.
Sympathetic stimulation of saliva is to facilitate respiration, whereas parasympathetic stimulation is to facilitate digestion.
Parasympathetic stimulation leads to acetylcholine release onto the salivary acinar cells. ACh binds to muscarinic receptors, specifically M3, and causes an increased intracellular calcium ion concentration. Increased calcium causes vesicles within the cells to fuse with the apical cell membrane leading to secretion. ACh also causes the salivary gland to release kallikrein, an enzyme that converts kininogen to lysyl-bradykinin. Lysyl-bradykinin acts upon blood vessels and capillaries of the salivary gland to generate vasodilation and increased capillary permeability, respectively. The resulting increased blood flow to the acini allows the production of more saliva. In addition, Substance P can bind to Tachykinin NK-1 receptors leading to increased intracellular calcium concentrations and subsequently increased saliva secretion. Lastly, both parasympathetic and sympathetic nervous stimulation can lead to myoepithelium contraction which causes the expulsion of secretions from the secretory acinus into the ducts and eventually to the oral cavity.
Sympathetic stimulation results in the release of norepinephrine. Norepinephrine binding to α-adrenergic receptors will cause an increase in intracellular calcium levels leading to more fluid vs. protein secretion. If norepinephrine binds β-adrenergic receptors, it will result in more protein or enzyme secretion vs. fluid secretion. Stimulation by norepinephrine initially decreases blood flow to the salivary glands due to constriction of blood vessels but this effect is overtaken by vasodilation caused by various local vasodilators.
Saliva production may also be pharmacologically stimulated by the so-called sialagogues. It can also be suppressed by the so-called antisialagogues.
Behaviour
Spitting
is the act of forcibly ejecting saliva or other substances from the mouth. In many parts of the world, it is considered rude and a social taboo, and has even been outlawed in many countries. In Western countries, for example, it has often been outlawed for reasons of public decency and attempting to reduce the spread of disease; however, these laws are often not strictly enforced. In Singapore, the fine for spitting may be as high as SGD$2,000 for multiple offenses, and one can even be arrested. In some other parts of the world, such as in China, expectoration is more socially acceptable, and spittoons are still a common appearance in some cultures. Some animals, even humans in some cases, use spitting as an automatic defensive maneuver. Camels are well known for doing this, though most domestic camels are trained not to.Because saliva can contain large amounts of virus copies in infected individuals, spitting in public places can pose a health hazard to the public.