Sporulation in Bacillus subtilis


Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore. B. subtilis can divide symmetrically to make two daughter cells, or asymmetrically, producing a single endospore that is resistant to environmental factors such as heat, desiccation, radiation and chemical insult which can persist in the environment for long periods of time. The endospore is formed at times of nutritional stress, allowing the organism to persist in the environment until conditions become favourable. The process of endospore formation has profound morphological and physiological consequences: radical post-replicative remodelling of two progeny cells, accompanied eventually by cessation of metabolic activity in one daughter cell and death by lysis of the other.

Overview

Commitment to sporulation

Although sporulation in B. subtilis is induced by starvation, the sporulation developmental program is not initiated immediately when growth slows due to nutrient limitation. A variety of alternative responses can occur, including the activation of flagellar motility to seek new food sources by chemotaxis, the production of antibiotics to destroy competing soil microbes, the secretion of hydrolytic enzymes to scavenge extracellular proteins and polysaccharides, or the induction of ‘competence’ for uptake of exogenous DNA for consumption, with the occasional side-effect that new genetic information is stably integrated. Sporulation is the last-ditch response to starvation and is suppressed until alternative responses prove inadequate. Even then, certain conditions must be met such as chromosome integrity, the state of chromosomal replication, and the functioning of the Krebs cycle.

Nature of regulation

Sporulation requires a great deal of time and also a lot of energy and is essentially irreversible, making it crucial for a cell to monitor its surroundings efficiently and ensure that sporulation is embarked upon at only the most appropriate times. The wrong decision can be catastrophic: a vegetative cell will die if the conditions are too harsh, while bacteria forming spores in an environment which is conducive to vegetative growth will be out competed. In short, initiation of sporulation is a very tightly regulated network with numerous checkpoints for efficient control.

Control points

Two transcriptional regulators, σH and Spo0A, play key roles in initiation of sporulation. Several additional proteins participate, mainly by controlling the accumulated concentration of Spo0A~P. Spo0A lies at the end of a series of inter-protein phosphotransfer reactions, Kin–Spo0F–Spo0B–Spo0A, termed as a ‘’. The regulation of these various factors controlling the accumulated concentration of Spo0A~P, and their interactions are described in detail in :
In the table below, the term 'Activators' refers to genes/proteins that ultimately result in initiation of sporulation and 'Repressors' refers to the ones that inhibit this initiation of sporulation.
ActivatorsRegulationRepressorsRegulation
KinATransfers phosphate to the Spo0FSdaBlocks autophosphorylation of KinA
KinBTransfers phosphate to the Spo0FKipIBlocks autophosphorylation of KinA
Spo0AActivates several key sporulation-specific genesSpo0ANegatively controls transcription of abrB
Spo0H Activates phrE geneComAActivates competence
Spo0BPhosphotransferase initiationSinRNegative regulation of kinB
Spo0FPhosphotransferase initiationRapA, RapB, RapE, and RapHDephosphorylation of Spo0F~P
ComAActivates phrASpo0E, YisI, and YnzDDephosphorylation of Spo0A~P
SinIAntagonist of SinRGTP-bound CodYInhibits rapA-phrA
KipAInhibits kipI geneAbrBEncodes a repressor of spo0H
PhrASuppresses dephosphorylation activity of RapAHprOverexpression inhibits sporulation
PhrEInhibits RapEDnaAOver expression of Sda
PhrHInhibits RapH

Homologous networks in eukaryotes

Spores form a part of the life cycles of a diverse range of organisms such as many bacteria, plants, algae, fungi and some protozoa. In Saccharomyces cerevisiae, the set of early genes activating sporulation is induced by Ime1 and a regulator of middle genes is Ndt80p. Similarly, in Dictyostelium discoideum, SDF-2 and cytokinins are secreted during late development to trigger signal transduction pathways that lead to an increase in the activity of the cAMP-dependent protein kinase, PKA, which triggers rapid encapsulation as well as ensuring spore dormancy.