Thomas Byron was born around 1610, and was the fifth of seven sons of Sir John Byron of Newstead Abbey, Nottinghamshire, and Anne Molyneux. The Byron family were influential land-owners in both Lancashire and Nottinghamshire, across which they owned thousands of acres of land. Byron's great-grandfather and grandfather both served as High Sheriff of Nottinghamshire, and his grandfather was also a Member of Parliament for the county in 1597. The family estate lessened during his grandfather's life, as he was forced to sell off parts of it to pay off his debts. Thomas married Katherine Braine, and had two children, Thomas and John, who both died in infancy. Katherine outlived Byron, and was buried at Westminster Abbey in 1676.
On the outbreak of the First English Civil War in 1642, Byron and his brothers were "all passionately the King's", according to the biographer George Yerby. Thomas Byron was appointed colonel of the Prince of Wales' cavalry regiment, and also commanded the Prince's troop, one of seven which made up the regiment. He was knighted by King Charles on 27 September, in Shrewsbury. At the Battle of Edgehill in October 1642, where the regiment fought on the Royalist's right wing, Byron saved the life of one of his men, Richard Bulstrode, by shooting a Parliamentarian soldier who was attacking Bulstrode. In late November, during the Royalist campaign in the Thames Valley, Byron and his older brother John occupied Fawley Court, the home of Bulstrode Whitelocke. The Royalist soldiers were ransacking the house, but Byron found Whitelocke's children hidden in the estate nearby, and protected them from harm, claiming that "it were a barbarous thing to hurt the pretty, innocent children". In the following January, the regiment was quartered in Oxfordshire, where they received a stipend of £252 per week. By the following year, Edward Hyde, 1st Earl of Clarendon, described Byron as the de facto commander of the Prince of Wales' regiment, although the position was officially held by Henry Clifford, 5th Earl of Cumberland. Clarendon called Byron a "very valuable and experience officer". In March 1643, at the Battle of Hopton Heath, the Royalists' commanding officer, the Earl of Northampton, was killed leading a cavalry attack. Byron led a second charge which overran and captured the enemy artillery, while driving their cavalry from the battlefield. Byron himself was injured, taking a wound to his thigh. After recovering, Byron was given orders to provide support to Lord Hopton, who after his campaign in the south-west, was preparing to assault Surrey and Sussex. Byron and his regiment joined forces with Hopton in November 1643.
Death
On 7 December 1643, Byron was attacked in Oxford by one of his own soldiers, Captain Hurst, over a pay dispute as he left his lodgings. The historian Peter Young speculates that Hopton may have been on leave, as the Prince of Wales' Regiment was still with Hopton. Hurst was executed the following week, while Byron died of the chest wound from the attack two months later, on 5 February 1644. He was buried four days later at Christ Church Cathedral, Oxford.