Toxicity
Toxicity is the degree to which a chemical substance or a particular mixture of substances can damage an organism. Toxicity can refer to the effect on a whole organism, such as an animal, bacterium, or plant, as well as the effect on a substructure of the organism, such as a cell or an organ such as the liver. By extension, the word may be metaphorically used to describe toxic effects on larger and more complex groups, such as the family unit or society at large. Sometimes the word is more or less synonymous with poisoning in everyday usage.
A central concept of toxicology is that the effects of a toxicant are dose-dependent; even water can lead to water intoxication when taken in too high a dose, whereas for even a very toxic substance such as snake venom there is a dose below which there is no detectable toxic effect. Considering the limitations of this dose-response concept, a novel has been proposed recently. DTI redefines drug toxicity, identifies hepatotoxic drugs, gives mechanistic insights, predicts clinical outcomes and has potential as a screening tool. Toxicity is species-specific, making cross-species analysis problematic. Newer paradigms and metrics are evolving to bypass animal testing, while maintaining the concept of toxicity endpoints.
Types
There are generally four types of toxic entities; chemical, biological, physical and radiation:- Chemical toxicants include inorganic substances such as, lead, mercury, hydrofluoric acid, and chlorine gas, and organic compounds such as methyl alcohol, most medications, and toxins. While some weakly radioactive substances, such as uranium, are also chemical toxicants, more strongly radioactive materials like radium are not, their harmful effects being caused by the ionizing radiation produced by the substance rather than chemical interactions with the substance itself.
- Disease-causing microorganisms and parasites are toxic in a broad sense but are generally called pathogens rather than toxicants. The biological toxicity of pathogens can be difficult to measure because the "threshold dose" may be a single organism. Theoretically one virus, bacterium or worm can reproduce to cause a serious infection. However, in a host with an intact immune system, the inherent toxicity of the organism is balanced by the host's ability to fight back; the effective toxicity is then a combination of both parts of the relationship. In some cases, e.g. cholera, the disease is chiefly caused by a nonliving substance secreted by the organism, rather than the organism itself. Such nonliving biological toxicants are generally called toxins if produced by a microorganism, plant, or fungus, and venoms if produced by an animal.
- Physical toxicants are substances that, due to their physical nature, interfere with biological processes. Examples include coal dust, asbestos fibers or finely divided silicon dioxide, all of which can ultimately be fatal if inhaled. Corrosive chemicals possess physical toxicity because they destroy tissues, but they're not directly poisonous unless they interfere directly with biological activity. Water can act as a physical toxicant if taken in extremely high doses because the concentration of vital ions decreases dramatically if there's too much water in the body. Asphyxiant gases can be considered physical toxicants because they act by displacing oxygen in the environment but they are inert, not chemically toxic gases.
- As already mentioned, radiation can have a toxic effect on organisms.
Measuring
Assessing all aspects of the toxicity of cancer-causing agents involves additional issues, since it is not certain if there is a minimal effective dose for carcinogens, or whether the risk is just too small to see. In addition, it is possible that a single cell transformed into a cancer cell is all it takes to develop the full effect.
It is more difficult to determine the toxicity of chemical mixtures than a pure chemical because each component displays its own toxicity, and components may interact to produce enhanced or diminished effects. Common mixtures include gasoline, cigarette smoke, and industrial waste. Even more complex are situations with more than one type of toxic entity, such as the discharge from a malfunctioning sewage treatment plant, with both chemical and biological agents.
The preclinical toxicity testing on various biological systems reveals the species-, organ- and dose-specific toxic effects of an investigational product. The toxicity of substances can be observed by studying the accidental exposures to a substance in vitro studies using cells/ cell lines in vivo exposure on experimental animals. Toxicity tests are mostly used to examine specific adverse events or specific end points such as cancer, cardiotoxicity, and skin/eye irritation. Toxicity testing also helps calculate the No Observed Adverse Effect Level dose and is helpful for clinical studies.
Classification
For substances to be regulated and handled appropriately they must be properly classified and labelled. Classification is determined by approved testing measures or calculations and have determined cut-off levels set by governments and scientists. Pesticides provide the example of well-established toxicity class systems and toxicity labels. While currently many countries have different regulations regarding the types of tests, numbers of tests and cut-off levels, the implementation of the Globally Harmonized System has begun unifying these countries.Global classification looks at three areas: Physical Hazards, Health Hazards and environmental hazards.
Health hazards
The types of toxicities where substances may cause lethality to the entire body, lethality to specific organs, major/minor damage, or cause cancer. These are globally accepted definitions of what toxicity is. Anything falling outside of the definition cannot be classified as that type of toxicant.Acute toxicity
Acute toxicity looks at lethal effects following oral, dermal or inhalation exposure. It is split into five categories of severity where Category 1 requires the least amount of exposure to be lethal and Category 5 requires the most exposure to be lethal. The table below shows the upper limits for each category.Method of administration | Category 1 | Category 2 | Category 3 | Category 4 | Category 5 |
Oral: measured in mg/kg of bodyweight | 5 | 50 | 300 | 2 000 | 5 000 |
Dermal: LD50 measured in mg/kg of bodyweight | 50 | 200 | 1 000 | 2 000 | 5 000 |
Gas Inhalation: LC50 measured in ppmV | 100 | 500 | 2 500 | 20 000 | Undefined |
Vapour Inhalation: LC50 measured in mg/L | 0.5 | 2.0 | 10 | 20 | Undefined |
Dust and Mist Inhalation: LC50 measured in mg/L | 0.05 | 0.5 | 1.0 | 5.0 | Undefined |
Note: The undefined values are expected to be roughly equivalent to the category 5 values for oral and dermal administration.
Other methods of exposure and severity
Skin corrosion and irritation are determined though a skin patch test analysis. This examines the severity of the damage done; when it is incurred and how long it remains; whether it is reversible and how many test subjects were affected.Skin corrosion from a substance must penetrate through the epidermis into the dermis within four hours of application and must not reverse the damage within 14 days. Skin irritation shows damage less severe than corrosion if: the damage occurs within 72 hours of application; or for three consecutive days after application within a 14-day period; or causes inflammation which lasts for 14 days in two test subjects. Mild skin irritation is minor damage within 72 hours of application or for three consecutive days after application.
Serious eye damage involves tissue damage or degradation of vision which does not fully reverse in 21 days. Eye irritation involves changes to the eye which do fully reverse within 21 days.
Other categories
- Respiratory sensitizers cause breathing hypersensitivity when the substance is inhaled.
- A substance which is a skin sensitizer causes an allergic response from a dermal application.
- Carcinogens induce cancer, or increase the likelihood of cancer occurring.
- Neurotoxicity is a form of toxicity in which a biological, chemical, or physical agent produces an adverse effect on the structure or function of the central and/or peripheral nervous system. It occurs when exposure to a substance – specifically, a neurotoxin or neurotoxicant– alters the normal activity of the nervous system in such a way as to cause permanent or reversible damage to nervous tissue.
- Reproductively toxic substances cause adverse effects in either sexual function or fertility to either a parent or the offspring.
- Specific-target organ toxins damage only specific organs.
- Aspiration hazards are solids or liquids which can cause damage through inhalation.
Environmental hazards
Common types of environmental hazards
- Water: detergents, fertilizer, raw sewage, prescription medication, pesticides, herbicides, heavy metals, PCBs
- Soil: heavy metals, herbicides, pesticides, PCBs
- Air: particulate matter, carbon monoxide, sulfur dioxide, nitrogen dioxide, asbestos, ground-level ozone, lead
Occupational hazards
Workers in various occupations may be at a greater level of risk for several types of toxicity, including neurotoxicity. The expression "Mad as a hatter" and the "Mad Hatter" of the book Alice in Wonderland derive from the known occupational toxicity of hatters who used a toxic chemical for controlling the shape of hats. Exposure to chemicals in the workplace environment may be required for evaluation by industrial hygiene professionals.Hazards for small businesses
Hazards from medical waste and prescription disposal
Hazards in the arts
Hazards in the arts have been an issue for artists for centuries, even though the toxicity of their tools, methods, and materials was not always adequately realized. Lead and cadmium, among other toxic elements, were often incorporated into the names of artist's oil paints and pigments, for example, "lead white" and "cadmium red".20th-century printmakers and other artists began to be aware of the toxic substances, toxic techniques, and toxic fumes in glues, painting mediums, pigments, and solvents, many of which in their labelling gave no indication of their toxicity. An example was the use of xylol for cleaning silk screens. Painters began to notice the dangers of breathing painting mediums and thinners such as turpentine. Aware of toxicants in studios and workshops, in 1998 printmaker Keith Howard published Non-Toxic Intaglio Printmaking which detailed twelve innovative Intaglio-type printmaking techniques including photo etching, digital imaging, acrylic-resist hand-etching methods, and introducing a new method of non-toxic lithography.
Mapping environmental hazards
There are many environmental health mapping tools. TOXMAP is a Geographic Information System from the Division of Specialized Information Services of the United States National Library of Medicine that uses maps of the United States to help users visually explore data from the United States Environmental Protection Agency's Toxics Release Inventory and Superfund programs. TOXMAP is a resource funded by the US Federal Government. TOXMAP's chemical and environmental health information is taken from NLM's Toxicology Data Networkand PubMed, and from other authoritative sources.
Aquatic toxicity
testing subjects key indicator species of fish or crustacea to certain concentrations of a substance in their environment to determine the lethality level. Fish are exposed for 96 hours while crustacea are exposed for 48 hours. While GHS does not define toxicity past 100 mg/l, the EPA currently lists aquatic toxicity as "practically non-toxic" in concentrations greater than 100 ppm.Exposure | Category 1 | Category 2 | Category 3 |
Acute | ≤ 1.0 mg/L | ≤ 10 mg/L | ≤ 100 mg/L |
Chronic | ≤ 1.0 mg/L | ≤ 10 mg/L | ≤ 100 mg/L |
Note: A category 4 is established for chronic exposure, but simply contains any toxic substance which is mostly insoluble, or has no data for acute toxicity.
Factors influencing toxicity
Toxicity of a substance can be affected by many different factors, such as the pathway of administration, the time of exposure, the number of exposures, the physical form of the toxicant, the genetic makeup of an individual, an individual's overall health, and many others. Several of the terms used to describe these factors have been included here.; Acute exposure: A single exposure to a toxic substance which may result in severe biological harm or death; acute exposures are usually characterized as lasting no longer than a day.
; Chronic exposure: Continuous exposure to a toxicant over an extended period of time, often measured in months or years; it can cause irreversible side effects.