Monica "Twinkletoes" Minasi, a motherless child of the LondonLimehouse district, is a brilliant young dancer who lives in poverty. She saves a crowd from abuse by the police through an impromptu performance, during which she meets Chuck Lightfoot, a champion fighter and older married man whose wife, Cissie, was the cause of the ruckus. Twinks finds herself slowly falling in love with Chuck but resists, because he is married and much older, but when he saves her from an attack one night she realizes that it is useless to fight her feelings. She dances at the head of the "Quayside Kids," a local dance group in a music hall run by Roseleaf, who has designs on the young girls that dance for him. Chuck's wife Cissie realizes that her husband had feelings for Twinks and, learning that Twink's dad is a burglar, exposes him to the police. Twinks is distraught when she learns the news that her father—whom she admired above all other people—is a criminal. Roseleaf takes her to his apartment and attempts to have his way with her, but she manages to escape. Cissie is killed in an accident, and, in despair, Twink throws herself into the river. She is rescued by Chuck and in his arms finds something to live for.
Scenario writerWinifred Dunn did research in San Francisco's Chinatown for the atmosphere of the film. The movie was based on the book Twinkletoes: A Tale of the Limehouse, by Thomas Burke. Like the book, the film's original story had a dramatic ending, with Twinks drowning in the river. Colleen's husband did not believe that the original ending would play as well as a happy ending, perhaps worried that the production might not be well received by audiences, as had been the case with the dramatic So Big. Two endings were filmed and exhibitors were allowed to pick which version they wanted to show. The audiences generally preferred the sad ending. This was Colleen's first "serious" film since her portrayal of Salena Peake in So Big. Coleen danced several of the numbers in the film herself. The film came between two of her comedies, as it was the studio's strategy to stagger her comedies and dramatic roles, so that the public would not become tired of any single genre.