| Team | Home city | Stadium | Capacity | 2015-16 season |
| Bul FC | Jinja | Kakindu Municipal Stadium | 1,000 | 6th in Ugandan Super League |
| Bright Stars FC | Matugga | Champions Stadium | 3,000 | 12th in Ugandan Super League |
| Express FC | Kampala | Mutesa II Stadium | 20,200 | 3rd in Ugandan Super League |
| Jinja Municipal Council Hippos FC | Jinja | Kakindu Municipal Stadium | 1,000 | 10th in Ugandan Super League |
| Kirinya-Jinja SSS FC | Jinja | Kyabazinga Stadium | 3,000 | FUFA Big League |
| Kampala Capital City Authority FC | Lugogo | Phillip Omondi Stadium | 5,600 | Ugandan Super League Champions |
| Lweza FC | Kampala | Mutesa II Stadium | 20,200 | 13th in Ugandan Super League |
| Onduparaka FC | Arua | Green Light Stadium | | FUFA Big League |
| Police FC | Wakiso | Kavumba Recreation Centre | 1,000 | 8th in Ugandan Super League |
| Proline FC | Kampala | Lugogo Stadium | 3,000 | FUFA Big League |
| SC Villa | Kampala | Nakivubo Stadium | 15,000 | 4th in Ugandan Super League |
| Sadolin Paints FC | Bugembe | Kyabazinga Stadium | 3,000 | 9th in Ugandan Super League |
| Soana FC | Wakiso | Kavumba Recreation Centre | 1,000 | 7th in Ugandan Super League |
| The Saints FC | Kampala | Mandela National Stadium | 45,202 | 11th in Ugandan Super League |
| Uganda Revenue Authority SC | Lugazi | Mehta Stadium | 1,000 | 5th in Ugandan Super League |
| Vipers SC | Kitende | St. Mary's Stadium | 20,000 | 2nd in Ugandan Super League |
Geoffrey Sserunkuma